Navigation Links
NIST seeks improved recovery of samples from biohazard events
Date:4/27/2011

It may not be as catchy a combination as "Miss Scarlet in the parlor with a revolver," but "polyester-rayon wipes in the field followed by saline-surfactant extraction and vortexing" is the most efficient solution to an important biological game of "Clue" deduced by researchers at the National Institute of Standards and Technology (NIST). As reported in a recent paper*, the NIST team studied different methods for collecting, extracting and quantifying microbial spores from indoor surfaces to estimate parameters that should be considered in the development of a standard biological sampling protocol. A precise and reliable recovery system is critical to evaluating the success of cleanup efforts following an accidental biohazard contamination or bioterrorist attack (such as the Bacillus anthracis spore-laden letters sent to Congress and elsewhere in 2001).

Technique matters. The NIST team found that the optimal method in their experiments was about 10 times more effective at recovering spores than the worst method, and that recovery dramatically improved by simply adding a surfactant to the extraction solution.

"A comprehensive look at the impact of protocol variables affecting the performance of spore recoveryespecially when dealing with serious threats such as anthraxis an important national homeland security priority," says lead researcher Sandra Da Silva. "In this study, we used Bacillus anthracis (Sterne), a vaccine strain, as a surrogate for Bacillus anthracis (Ames), the causative agent of the anthrax disease, in order to learn how to improve the low efficiency of current protocols."

Possible explanations for the poor results, Da Silva says, include using wipes made from materials that do not attract bacteria well or won't let go of collected bacteria, extraction liquids that fail to completely loosen spores from the wipes and get them into solution, or inadequate dissociation methods that leave spores cli
'/>"/>

Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Virginia Tech researcher seeks to use electrical stimulation to give voice to stroke patients
2. Overseas lab seeks US weed control recruits
3. LOreal Fellowship winner seeks to understand breast cancer
4. Europe seeks alternatives to natural latex from Asia
5. The white stuff: Marine lab team seeks to understand coral bleaching
6. Critical Zone Observatory seeks to answer climate change questions
7. International research team seeks to unravel flatworm regeneration
8. Researcher seeks to turn stem cells into blood vessels
9. WWF seeks innovative solutions to bycatch through worldwide competition
10. Recycling perlite: New, improved method saves resources
11. Detecting pathogens in waterways: An improved approach
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
NIST seeks improved recovery of samples from biohazard events
(Date:5/21/2015)... 21, 2015 According to ... Solutions (Hardware, Software, Services), by Applications (Surveillance (Airborne, ... by End-Users (Military & Defense & Commercial) - ... Imaging Market is expected to grow from $7252.0 ... at a Compound Annual Growth Rate (CAGR) of ...
(Date:5/20/2015)... 20, 2015 ... announced the addition of the  "5-year ... Recognition Market"  report to their offering.  ... service analyses trends in the iris ... commercial sectors, globally. Despite hardware pricing ...
(Date:5/19/2015)... May 18, 2015 Fingerprint Cards (FPC) ... FPC1025, FPC1145, FPC1155 and FPC1035 from one if its module ... and including Q3 2015 and the sensors will be used ... order values for 2015 hereby amount to 740 MSEK to ... of 140 MSEK and a number of smaller orders not ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2
... research team has developed a process that removes a ... team has programmed a photosynthetic microbe to self-destruct, making ... potentially less costly. "The real costs involved ... turning them into fuel," said Roy Curtiss, director of ...
... , TORONTO, Dec. 7 ... the assets of the healthcare communications company previously operating as ... move forward under new leadership and funding. , IgeaCare Solutions ... Chief Executive Officer. Under his direction, the new company will ...
... air and water pollution along the Athabasca River and ... say pollution levels have increased as a direct result ... biological sciences professor David Schindler was part of the ... study and found high levels of Polycyclic Aromatic Compounds. ...
Cached Biology News:Self-destructing bacteria improve renewable biofuel production 2Self-destructing bacteria improve renewable biofuel production 3IgeaCare Solutions Inc. Emerges Under New Leadership and Funding 2
(Date:5/28/2015)... 2015 Research and Markets( http://www.researchandmarkets.com/research/6cg3zw/cell_therapy ) ... report "Cell Therapy - Technologies, Markets and ... report describes and evaluates cell therapy technologies and ... important role in the practice of medicine. Hematopoietic ... bone marrow transplants. Role of cells in drug ...
(Date:5/28/2015)... CARLOS, Calif. , May 28, 2015 /PRNewswire/ ... testing, today announced a study published in ... the Panorama single-nucleotide polymorphism (SNP)-based non-invasive prenatal test ... the first trimester. A study ... that complete molar pregnancies occur in about 1 ...
(Date:5/28/2015)... , May 28, 2015 RXi Pharmaceuticals Corporation ... discovering and developing innovative therapeutics, primarily in the areas ... a public offering of 26 million units at a ... gross proceeds of approximately $10.4 million. Each unit consists ... 13-month overallotment purchase right to purchase 0.50 of a ...
(Date:5/28/2015)... 2015 The Fertility Centers of New ... Danielle Vitiello has taken on a new and expanded ... will provide guidance, leadership, oversight and quality assurance for ... Vitiello among our team of experienced reproductive endocrinologists,” said ... A. Hill, M.D. “Her expertise and compassionate, individualized care ...
Breaking Biology Technology:Global Cell Therapy Market 2015-2020 - Technologies, Markets and Companies 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 3RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 3Danielle Vitiello, MD of Fertility Centers of New England, Named New Medical Director 2
... MedCap press releases contain false and misleading ... 25 /PRNewswire-FirstCall/-- VaxGen,Inc. (OTC Bulletin Board: VXGN.OB), ... ), Dear Stockholders, On March ... stockholder in,VaxGen, issued a press release entitled ...
... BEIJING, March 25 /Xinhua-PRNewswire/ -- Sinovac Biotech Ltd. (Amex:,SVA), ... that its,Annual General Meeting originally scheduled for March 25, ... the March 25, 2008 Annual General Meeting, the required ... present. In accordance with,Antigua law, the meeting is adjourned ...
... N.J., March 24 Osteotech, Inc.,(Nasdaq: OSTE ... biologic solutions for,regenerative medicine, announced today that the ... validity of its U.S. Patent No.,5,676,146. The ,146 ... demineralized bone, with a radiopaque material, such as ...
Cached Biology Technology:VaxGen Issues Rebuttal to MedCap Letter 2VaxGen Issues Rebuttal to MedCap Letter 3VaxGen Issues Rebuttal to MedCap Letter 4VaxGen Issues Rebuttal to MedCap Letter 5VaxGen Issues Rebuttal to MedCap Letter 6VaxGen Issues Rebuttal to MedCap Letter 7VaxGen Issues Rebuttal to MedCap Letter 8VaxGen Issues Rebuttal to MedCap Letter 9VaxGen Issues Rebuttal to MedCap Letter 10VaxGen Issues Rebuttal to MedCap Letter 11Sinovac Announces Adjournment of Annual General Meeting 2U.S. Patent Office Confirms Validity of Osteotech's U.S. Patent No. 5,676,146 2
... PDS-1000 He biolistic system enables 7-10 times more ... It fits into the shocking chamber of the ... shock wave over 7 microcarriers. By uniformly spreading ... larger area, the system maximizes the number of ...
Kit Components: Anti-Acetylated Histone H3 Salmon Sperm DNA/Protein A Agarose All necessary buffers. ...
... mitochondrial transmembrane potential is one of ... following induction of apoptosis. The ... lipophilic cation, termed as MitoLight, as ... a mitochondrial dye that stains mitochondria ...
Liquid. 50 μg/ml in PBS, pH 7.4. PROTECT FROM LIGHT. A convenient form of propidium iodide useful for flow cytometry studies. RTECS SF7949600, CAS 25535-16-4, M.W. 668.4. ...
Biology Products: