Navigation Links
NIST nanofluidic 'multi-tool' separates and sizes nanoparticles

A wrench or a screwdriver of a single size is useful for some jobs, but for a more complicated project, you need a set of tools of different sizes. Following this guiding principle, researchers at the National Institute of Standards and Technology (NIST) have engineered a nanoscale fluidic device that functions as a miniature "multi-tool" for working with nanoparticlesobjects whose dimensions are measured in nanometers, or billionths of a meter.

First introduced in March 2009 (see "NIST-Cornell Team Builds World's First Nanofluidic Device with Complex 3-D Surfaces", the device consists of a chamber with a cascading "staircase" of 30 nanofluidic channels ranging in depth from about 80 nanometers at the top to about 620 nanometers (slightly smaller than an average bacterium) at the bottom. Each of the many "steps" of the staircase provides another "tool" of a different size to manipulate nanoparticles in a method that is similar to how a coin sorter separates nickels, dimes and quarters.

In a new article in the journal Lab on a Chip*, the NIST research team demonstrates that the device can successfully perform the first of a planned suite of nanoscale tasksseparating and measuring a mixture of spherical nanoparticles of different sizes (ranging from about 80 to 250 nanometers in diameter) dispersed in a solution. The researchers used electrophoresisthe method of moving charged particles through a solution by forcing them forward with an applied electric fieldto drive the nanoparticles from the deep end of the chamber across the device into the progressively shallower channels. The nanoparticles were labeled with fluorescent dye so that their movements could be tracked with a microscope.

As expected, the larger particles stopped when they reached the steps of the staircase with depths that matched their diameters of around 220 nanometers. The smaller particles moved on until they, too, were restricted from moving into shallower channels at depths of around 110 nanometers. Because the particles were visible as fluorescent points of light, the position in the chamber where each individual particle was stopped could be mapped to the corresponding channel depth. This allowed the researchers to measure the distribution of nanoparticle sizes and validate the usefulness of the device as both a separation tool and reference material. Integrated into a microchip, the device could enable the sorting of complex nanoparticle mixtures, without observation, for subsequent application. This approach could prove to be faster and more economical than conventional methods of nanoparticle sample preparation and characterization.

The NIST team plans to engineer nanofluidic devices optimized for different nanoparticle sorting applications. These devices could be fabricated with tailored resolution (by increasing or decreasing the step size of the channels), over a particular range of particle sizes (by increasing or decreasing the maximum and minimum channel depths), and for select materials (by conforming the surface chemistry of the channels to optimize interaction with a specific substance). The researchers are also interested in determining if their technique could be used to separate mixtures of nanoparticles with similar sizes but different shapesfor example, mixtures of tubes and spheres.


Contact: Michael E. Newman
National Institute of Standards and Technology (NIST)

Related biology news :

1. NIST-Cornell team builds worlds first nanofluidic device with complex 3-D surfaces
2. Brain innately separates living and non-living objects for processing
3. New method separates cancer cells from normal cells
4. TEEB study leader emphasizes urgent need for action on biodiversity loss
5. UCLA researchers reconstitute enzyme that synthesizes cholesterol drug lovastatin
6. Sea Grant report synthesizes recent research on New Yorks clams
7. Iowa State chemist synthesizes carbohydrates, launches startup company
8. Nanoparticle toxicity doesnt get wacky at the smallest sizes
9. New catalyst of platinum nanoparticles could lead to conk-out free, stable fuel cells
10. Nanoblasts from laser-activated nanoparticles move molecules, proteins and DNA into cells
11. Researchers use nanoparticles as destructive beacons to zap tumors
Post Your Comments:
Related Image:
NIST nanofluidic 'multi-tool' separates and sizes nanoparticles
(Date:10/29/2015)... , Oct. 29, 2015   MedNet Solutions ... the entire spectrum of clinical research, is pleased to ... High Tech Association (MHTA) as one of only three ... the "Software – Small and Growing" category. The Tekne Awards ... who have shown superior technology innovation and leadership. ...
(Date:10/29/2015)... 29, 2015  Connected health pioneer, Joseph C. ... of technology-enabled health and wellness, and the business opportunities ... The Internet of Healthy Things . Long ... even existed, Dr. Kvedar, vice president, Connected Health, Partners ... delivery, moving care from the hospital or doctor,s office ...
(Date:10/27/2015)... , October 27, 2015 ... Semantic Gaze Mapping technology (ASGM) automatically maps data from ... Tracking Glasses , so that they can be ... --> Munich, Germany , October 28-29, ... maps data from mobile eye tracking videos created with ...
Breaking Biology News(10 mins):
(Date:11/25/2015)... 25, 2015 2 nouvelles études permettent ... les différences entre les souches bactériennes retrouvées dans la ... des êtres humains . Ces recherches  ouvrent une nouvelle ... prise en charge efficace de l,un des problèmes ... chats .    --> 2 nouvelles études ...
(Date:11/25/2015)... 25, 2015  Neurocrine Biosciences, Inc. (Nasdaq: NBIX ... and CEO of Neurocrine Biosciences, will be presenting at ... New York . .   ... approximately 5 minutes prior to the presentation to download ... presentation will be available on the website approximately one ...
(Date:11/25/2015)... DIEGO , Nov. 25, 2015 Orexigen® ... management will participate in a fireside chat discussion at ... New York . The discussion is scheduled ... .  A replay will be ... Media Contact:McDavid Stilwell  , Julie NormartVP, Corporate Communications ...
(Date:11/24/2015)... Malaysia , Nov. 24, 2015  Asia-Pacific ... contract research organisation (CRO) market. The trend of ... in lower margins but higher volume share for ... capacity and scale, however, margins in the CRO ... Organisation (CRO) Market ( ), finds ...
Breaking Biology Technology: