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NIH ramps up Human Microbiome Project
Date:9/7/2010

rectum and colon, are treated with surgical removal of the colon, followed by construction of a pouch to assist the patient with bowel movements. For unknown reasons, some patients develop inflammation of the pouch, necessitating further surgery. This research will determine whether the composition of the pouch microbiome influences the onset and progression of pouch inflammation.

Historically, microbes have been studied in the laboratory as cultures of isolated species. Microbial growth is dependent upon a very specific natural environment, and it is often difficult to duplicate these conditions in a laboratory. Therefore, the development of novel and innovative technologies is needed to improve and refine the identification and characterization of the microbes that comprise the complex mixtures found in and on our bodies.

The principal investigators who will develop new technologies, their approximate funding levels and their areas of research are:
Emma Allen-Vercoe, Ph.D., University of Guelph, Ontario
$179,000 (2 years)
A Method to Sort and Enrich the as-yet Uncultured Bacterial Species from the Human Distal Gut

Ronald Davis, Ph.D., Stanford University, Palo Alto, Calif.
$440,000 (2 years)
Isolation, Selection and Polony Amplification of Single Cells in a Gel Matrix

David Fredricks, M.D., Fred Hutchinson Cancer Research Center, Seattle.
$2 million (3 years)
Novel Cultivation Methods for the Domestication of Vaginal Bacteria

Rustem Ismagilov, Ph.D., University of Chicago
$1.1 million (3 years)
Confining Single Cells to Enhance and Target Cultivation of Human Microbiome

Kim Lewis, Ph.D., Northeastern University, Boston
$1.2 million (3 years)
Culturing Uncultivatable Gut Microorganisms

John Nelson, Ph.D., General Electric Global Research, Niskayuna, N.Y.
$538,000 (2 years)
Tools for Human Microbiome Studies

Analysis of the seq
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Contact: Geoff Spencer
spencerg@mail.nih.gov
301-402-0911
NIH/National Human Genome Research Institute
Source:Eurekalert

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