Navigation Links
NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays

Cambridge, MA January 7, 2009 Phylonix Pharmaceuticals, Inc. today announced that it has been awarded a $1,250,369 Phase II Small Business Innovation Research Grant (SBIR) from the National Institute of General Medical Sciences of the National Institutes of Health (NIH) to develop in vivo zebrafish assays for assessing drug effects on Cytochrome (CYP) P450s. CYP profiling, a critical step in drug development, is required by the Food and Drug Administration (FDA) for all new drug approvals.

"This grant, 'Zebrafish Cytochrome P450 Assays for Assessing Drug Metabolism and Drug Safety,' will support the Phylonix research program for assessing potential drug-to-drug interactions (DDIs) during preclinical research," commented Patricia McGrath, Phylonix President and Chief Executive Officer. "A rapid and robust whole zebrafish CYP assay, amenable to automation in multiwell plate formats, will accelerate drug metabolism and safety profiling and reduce the possibility of costly late-stage drug development failures or product market withdrawal after commercialization," she continued.

Background on the Technical Approach

Many clinically relevant drug-to-drug interactions (DDIs) are associated with inhibition and/or induction of a CYP enzyme, such as 3A4. Modification of CYP activities can profoundly affect therapeutic efficacy and lead to life-threatening toxicity. Examples of costly high-profile drug withdrawals due to CYP-related DDIs include the antihistamines terfenadine and astemizole, the antihypertensive mibefradil and statins such as cerivastatin. A validated, simple, cost-effective in vivo CYP assay will be useful for profiling drug metabolism prior to performing expensive mammalian testing. Because of their genetic and physiological similarity to humans, zebrafish have been shown to be an efficient, predictive animal model for assessing drug metabolism and drug safety.

Advantages of Zebrafish for Compound Screening

Laboratory animals are critical for defining the mechanisms of drug activity and for testing therapeutic regimens; however, only a few useful models for assessing drug metabolism have been developed. Zebrafish have several important advantages for drug screening: they are small, inexpensive to maintain, and easily bred in large numbers. Zebrafish eggs are externally fertilized and each mating produces 100-200 eggs. Single zebrafish can be maintained in fluid volumes as small as 100 microliters for the first six days of development. Chemicals can then be added directly to the fish water, permeating the intact embryos. Standard microtiter plate readers can be used for quantitation, making this format particularly attractive for high-throughput drug screening.


Contact: Ed Olear
Phylonix Pharmaceuticals

Related biology news :

1. Life Sciences Discovery Fund awards health research program grants
2. REGiMMUNE receives $12 million in grants to develop transplant and allergy drugs
3. NIST awards $24M in grants for new research facilities
4. Northwest climate change is target of $3.2M in grants to University of Oregon
5. Dunn Foundation gives Rice $3M for collaborative research grants
6. National Science Foundation grants Clemson professors award to develop nanoprobes
7. Exciting new companies at NJITs small biz incubator get 100K in grants
8. NSF, NIH award Ecology of Infectious Disease grants
9. NSF awards two grants to Stevens for nano/micro device fabrication and nano sensor development
10. UT Knoxville wins 2 $3M National Science Foundation research and education grants
11. Mellon grants benefit Academy specimen collection
Post Your Comments:
(Date:11/17/2015)... , November 17, 2015 Paris ...   --> Paris from 17 th ... DERMALOG, the biometrics innovation leader, has invented the first combined ... on the same scanning surface. Until now two different scanners ... one scanner can capture both on the same surface. ...
(Date:11/12/2015)... golden retriever that stayed healthy despite having the gene ... new lead for treating this muscle-wasting disorder, report scientists ... and Harvard and the University of São Paolo in ... pinpoints a protective gene that boosts muscle regeneration, ... Children,s lab of Lou Kunkel , PhD, is ...
(Date:11/10/2015)... NEW YORK , Nov. 10, 2015 /PRNewswire/ ... refers to behavioral biometrics that helps to identify ... prevent fraud. Signature is considered as the secure ... for the identification of a particular individual because ... offers more accurate results especially when dynamic signature ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... ... November 24, 2015 , ... International Society ... one of the premier annual events for pharmaceutical manufacturing: 2015 Annual Meeting. The ... where ISPE hosted the largest number of attendees in more than a decade. ...
(Date:11/24/2015)... PUNE, India , November 24, 2015 ... to a new market research report "Oligonucleotide Synthesis Market ... Equipment), Application (PCR, Gene Synthesis, Diagnostic, DNA, RNAi), End-User ... to 2020", published by MarketsandMarkets, the market is expected ... 1,078.1 Million in 2015, at a CAGR of 10.1% ...
(Date:11/24/2015)... 24, 2015 SHPG ) announced today that ... Piper Jaffray 27 th Annual Healthcare Conference in ... at 8:30 a.m. EST (1:30 p.m. GMT). --> SHPG ... will participate in the Piper Jaffray 27 th Annual Healthcare ... Tuesday, December 1, 2015, at 8:30 a.m. EST (1:30 p.m. GMT). ...
(Date:11/24/2015)... QUEBEC CITY , Nov. 24, 2015 /PRNewswire/ ... (the "Company") announced today that the remaining 11,000 ... Common Share Purchase Warrants (the "Series B Warrants") ... agreement were exercised on November 23, 2015, which ... Common Shares.  After giving effect to the issuance ...
Breaking Biology Technology: