NHGRI seeks DNA sequencing technologies fit for routine laboratory and m...(y of Pennsylvania Philadelphia $820000 (...)

This team of researchers will address several current obstacles to achieving nanopore-based DNA sequencing by using nanoelectrodes to sense and manipulate molecules passing through the nanopore, and by integrating microfluidics to actively transport DNA molecules to the nanopore. Developments will be made available to other groups working to create nanopore-based DNA sequencers.

Di Gao, Ph.D., University of Pittsburgh, Pennsylvania
$370,000 (2 years)
DNA Sequencing-At-A-Stretch

This team will lay the groundwork to prove basic principles for a technology where DNA strands are pulled away from a solid surface when stretched by an electric field. When the stretching force exceeds a certain value, which is proportional to DNA length, the DNA strand would be released from the surface and detected by fluorescence. The order in which strands are released allows the instrument to identify the sequence of base pairs.

Xiaohua Huang, Ph.D., University of California, San Diego, La Jolla
$2.5 million (4 years)
Genome Sequencing by Natural DNA Synthesis on Amplified DNA Clones

Building on recent advances in sequencing by synthesis in several laboratories, and this lab's advances in preparing very large numbers of sequencing templates on a surface, this project aims to develop an instrument and protocols to improve DNA sequence quality and speed while lowering cost, and develop methods for genome assembly from short sequence reads.

Jiali Li, Ph.D., University of Arkansas, Fayetteville
$830,000 (3 years)
Exploration of Solid-state Nanopore Reading Labeled Linear DNA Sequence

The goal of most nanopore-based sequencing platforms is to be able to sequence DNA without having to label or copy the nucleotides. However, this team will conduct basic resear
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