Navigation Links
NC State researchers get to root of parasite genome

North Carolina State University scientists and colleagues have completed the genome sequence and genetic map of one of the world's most common and destructive plant parasites - Meloidogyne hapla, a microscopic, soil-dwelling worm known more commonly as the northern root-knot nematode.

The research could help lead to a new generation of eco-friendly tools to manage the ubiquitous parasitic worm, which, along with other species of root-knot nematode, causes an estimated $50 billion in crop and plant damage yearly, says Dr. Charles Opperman, professor of plant pathology at NC State, co-director of the Center for the Biology of Nematode Parasitism and the corresponding author on a scientific paper describing the research. The resulting sequence data has been deposited in public databases, so other researchers interested in the root-knot nematode - how it develops, establishes a host-parasite interaction or evades host defenses, for example - are now able to use the map of the parasite's genes as a tool to discover more specific information about the parasite.

The northern root-knot nematode is the smallest multicellular animal genome completely sequenced, says Dr. David McK. Bird, professor of plant pathology at NC State, co-director of the Center for the Biology of Nematode Parasitism and a co-author of the paper.

The study is published online this week in Proceedings of the National Academy of Sciences. Researchers from the University of California, Davis; the University of California, Berkeley; and the Joint Genome Institute also contributed to the research.

The northern root-knot nematode has been developed into a key model species in the study of plant-parasitic nematodes, and the completion of the genome sequence will further empower researchers to ask highly specific questions about the evolution and nature of parasitism. "A key facet to making M. hapla the premier model species for plant-parasitic nematodes is the development of a genetic map by our colleague, Dr. Valerie Williamson, at the University of California-Davis. The combination of a complete genome sequence with the genetic map makes this a unique and powerful system for the in-depth study of nematode-host interaction" Opperman says.

Besides being extremely important for the development of new and effective management strategies, the researchers say that the information gleaned from the genome sequence and genetic map will help scientists learn more about what they call the "themes of parasitism."

"All parasites have to do the same things to infect their hosts, whether the hosts are plants, animals or humans," Bird says. "Plants offer an advantage over those systems because they are easier to manipulate experimentally, and enable us to perform detailed experiments not easily done in animals, and not possible in humans."

The study shows that M. hapla has a somewhat smaller genome when compared with other microscopic worms like Caenorhabditis elegans, one of the models of scientific studies of animals. The northern root-knot nematode genome might be smaller, the researchers say, because the inside of the host plant's root provides an isolated environment compared to the soil.

"Having 99 percent of the genome sequenced allows you to not only know what's there, but to compare it to other nematodes to see what's missing from this genome," Bird says. "Finding potential Achilles' heels, what the nematode is getting from the plant and how is it really interacting with the plant are all more possible now."

The genome's reduced size made it easier to assemble the sequence, Opperman says. "In combination with an extensive database of plant parasitic nematode expressed genes from a previous project led by our Center for the Biology of Nematode Parasitism, this system provides a powerful platform for study of these important parasites," he added.

Although M. hapla was previously not known to be as widespread as other species of root-knot nematode, the cool-climate worm is now taking root in warmer climes, perhaps due to global climate change. The worm has been detected recently in Ugandan soils and other tropical and subtropical regions, for example. The expansion of range to new climates makes finding ways of controlling it even more critical, the researchers say.


Contact: Mick Kulikowski
North Carolina State University

Related biology news :

1. Iowa State engineer works to clean and improve engine performance
2. Nitrate concentrations of ground water increasing in many areas of the United States
3. K-State professors USDA research shows mad cow disease also caused by genetic mutation
4. Iowa State wins $18.5M grant to create NSF Center for Biorenewable Chemicals
5. Stem cell research puts interstate rivalry on hold
6. NC State first university in nation to offer canine bone marrow transplants
7. Height linked to risk of prostate cancer development and progression
8. Iowa State University researcher shows proteins have controlled motions
9. Rapid test for pathogens developed by K-State researchers
10. Why a common treatment for prostate cancer ultimately fails
11. Sweets make young horses harder to train in Montana State study
Post Your Comments:
(Date:6/27/2016)... June 27, 2016 Research and Markets has ... 2016-2020" report to their offering. ... America to grow at a CAGR of 12.28% during ... based on an in-depth market analysis with inputs from industry experts. ... the coming years. The report also includes a discussion of the ...
(Date:6/22/2016)... , June 22, 2016  The American College of ... Trade Show Executive Magazine as one of the fastest-growing ... May 25-27 at the Bellagio in Las Vegas ... the highest percentage of growth in each of the following ... exhibiting companies and number of attendees. The 2015 ACMG Annual ...
(Date:6/22/2016)... ANGELES , June 22, 2016 /PRNewswire/ ... identity management and verification solutions, has partnered ... edge software solutions for Visitor Management, Self-Service ... provides products that add functional enhancements ... partnership provides corporations and venues with an ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... -- Alex,s Lemonade Stand Foundation (ALSF), a leading national childhood ... state-of-the-art bioinformatics lab, using ,big data, to advance the ... Liz Scott , co-executive director of ALSF and ... Washington, D.C. , hosted by Vice ... of pediatric cancer research and awareness. ...
(Date:6/27/2016)... June 27, 2016  Global demand for enzymes ... through 2020 to $7.2 billion.  This market includes ... cleaning products, biofuel production, animal feed, and other ... and biocatalysts). Food and beverages will remain the ... increasing consumption of products containing enzymes in developing ...
(Date:6/27/2016)... June 27, 2016 /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" ... by its major shareholders, Clean Technology Fund I, LP ... States based venture capital funds which together ... (on a fully diluted, as converted basis), that they ... their entire equity holdings in Biorem to TUS Holdings ...
(Date:6/27/2016)... , June 27, 2016  Sequenom, Inc. (NASDAQ: ... enabling healthier lives through the development of innovative products ... the United States denied its ... the claims of Sequenom,s U.S. Patent No. 6,258,540 (",540 ... criteria established by the Supreme Court,s Mayo Collaborative Services ...
Breaking Biology Technology: