Navigation Links
NC State develops more precise genetic 'off switches'
Date:10/28/2010

Researchers at North Carolina State University have found a way to "cage" genetic off switches in such a way that they can be activated when exposed to UV light. Their technology gives scientists a more precise way to control and study gene function in localized areas of developing organisms.

The off switches, called morpholino oligonucleotides, are like short snippets of DNA that, when introduced into cells, bind to target RNA molecules, effectively turning off specific genes. Morpholinos have been used as genetic switches in many animal models, including the zebrafish embryo. However, morpholinos are distributed throughout dividing cells in a developing embryo, thereby turning off the specific gene everywhere. Moreover, they are active right after injection, silencing the targeted gene throughout development of the organism. Such uncontrolled genetic disruption makes studying tissue-specific and time-specific gene function difficult.

Dr. Alex Deiters, associate professor of chemistry, Dr. Jeffrey Yoder, associate professor of molecular biomedical sciences, and a team of NC State researchers developed a new methodology to turn off genes at a specific time and in a specific region of an organism. Deiters' team devised a way to synthesize morpholinos that would only bind with RNA molecules after a brief exposure to UV light, effectively "caging" the morpholino and providing a method for precisely controlling the genetic off switch. Yoder's team then tested the new photo-caged morpholinos in a zebrafish model and confirmed that they performed as expected: the caged morpholinos did not disrupt gene function unless the embryos were briefly exposed to UV light.

The researchers' results appear online in the Journal of the American Chemical Society.


'/>"/>

Contact: Tracey Peake
tracey_peake@ncsu.edu
919-515-6142
North Carolina State University
Source:Eurekalert

Page: 1

Related biology news :

1. NC State researchers get to root of parasite genome
2. L-1 Identity Solutions Receives $5.9 Million Drivers License Contract Expansion from the State of Mississippi
3. NC State takes lead in crime scene investigation training
4. Penn State receives new NASA astrobiology grant
5. Penn State researcher wins Entomological Society of America award
6. Paradigm Tactical Products to be Largest Distributor of Metal/Radiation Detection Wands in United States
7. Iowa State researcher develops new treatment method for canine eye diseases
8. L-1 Identity Solutions Selected by the State of New York as the Winning Bidder to Provide Enrollment Services for a Contract Estimated at Up to $250 Million
9. State fund advances titanium powder research, 9 other Iowa State projects
10. 15-state Southern obesity summit to focus on deadly epidemic
11. Montana State University researchers find gene that regulates molds resistance to drugs
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/11/2017)... 2017 No two people are believed ... New York University Tandon School of Engineering and ... that partial similarities between prints are common enough ... phones and other electronic devices can be more ... lies in the fact that fingerprint-based authentication systems ...
(Date:4/5/2017)... April 5, 2017  The Allen Institute for Cell ... Explorer: a one-of-a-kind portal and dynamic digital window into ... data, the first application of deep learning to create ... cell lines and a growing suite of powerful tools. ... these and future publicly available resources created and shared ...
(Date:4/4/2017)... YORK , April 4, 2017   EyeLock ... today announced that the United States Patent and Trademark ... patent broadly covers the linking of an iris image ... same transaction) and represents the company,s 45 th ... latest patent is very timely given the multi-modal biometric ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... ... AMRI, a global contract research, development and manufacturing organization ... quality of life, will now be offering its impurity solutions as a stand-alone ... for all new drug products, including the finalization of ICH M7 earlier this ...
(Date:10/11/2017)... ... October 11, 2017 , ... ComplianceOnline’s Medical Device Summit is ... and 8th June 2018 in San Francisco, CA. The Summit brings together current and ... distinguished CEOs, board directors and government officials from around the world to address key ...
(Date:10/11/2017)... (PRWEB) , ... October 11, 2017 , ... ... compared the implantation and pregnancy rates in frozen and fresh in vitro ... of progesterone and maternal age to IVF success. , After comparing the results ...
(Date:10/10/2017)... ... 10, 2017 , ... Dr. Bob Harman, founder and CEO of VetStem ... The event entitled “Stem Cells and Their Regenerative Powers,” was held ... Harman, DVM, MPVM was joined by two human doctors: Peter B. Hanson, M.D., Chief ...
Breaking Biology Technology: