Navigation Links
NC State develops more precise genetic 'off switches'
Date:10/28/2010

Researchers at North Carolina State University have found a way to "cage" genetic off switches in such a way that they can be activated when exposed to UV light. Their technology gives scientists a more precise way to control and study gene function in localized areas of developing organisms.

The off switches, called morpholino oligonucleotides, are like short snippets of DNA that, when introduced into cells, bind to target RNA molecules, effectively turning off specific genes. Morpholinos have been used as genetic switches in many animal models, including the zebrafish embryo. However, morpholinos are distributed throughout dividing cells in a developing embryo, thereby turning off the specific gene everywhere. Moreover, they are active right after injection, silencing the targeted gene throughout development of the organism. Such uncontrolled genetic disruption makes studying tissue-specific and time-specific gene function difficult.

Dr. Alex Deiters, associate professor of chemistry, Dr. Jeffrey Yoder, associate professor of molecular biomedical sciences, and a team of NC State researchers developed a new methodology to turn off genes at a specific time and in a specific region of an organism. Deiters' team devised a way to synthesize morpholinos that would only bind with RNA molecules after a brief exposure to UV light, effectively "caging" the morpholino and providing a method for precisely controlling the genetic off switch. Yoder's team then tested the new photo-caged morpholinos in a zebrafish model and confirmed that they performed as expected: the caged morpholinos did not disrupt gene function unless the embryos were briefly exposed to UV light.

The researchers' results appear online in the Journal of the American Chemical Society.


'/>"/>

Contact: Tracey Peake
tracey_peake@ncsu.edu
919-515-6142
North Carolina State University
Source:Eurekalert

Page: 1

Related biology news :

1. NC State researchers get to root of parasite genome
2. L-1 Identity Solutions Receives $5.9 Million Drivers License Contract Expansion from the State of Mississippi
3. NC State takes lead in crime scene investigation training
4. Penn State receives new NASA astrobiology grant
5. Penn State researcher wins Entomological Society of America award
6. Paradigm Tactical Products to be Largest Distributor of Metal/Radiation Detection Wands in United States
7. Iowa State researcher develops new treatment method for canine eye diseases
8. L-1 Identity Solutions Selected by the State of New York as the Winning Bidder to Provide Enrollment Services for a Contract Estimated at Up to $250 Million
9. State fund advances titanium powder research, 9 other Iowa State projects
10. 15-state Southern obesity summit to focus on deadly epidemic
11. Montana State University researchers find gene that regulates molds resistance to drugs
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/11/2014)... new report from the Wildlife Conservation Society shows ... help economically valuable species such as lobster, conch, ... help re-colonize nearby reef areas. , The reporttitled ... systematic review of research literature from no-take areas ... Dr. Craig Dahlgren, a recognized expert in marine ...
(Date:7/11/2014)... new professorship in tissue engineering to promote innovative ... and the Marine Biological Laboratory, supported by a ... Bell Foundation., The Eugene Bell Professorship in Tissue ... Engineering. That endowed chair holder also will direct ... Center for Regenerative Biology and Tissue Engineering, and ...
(Date:7/11/2014)... Shenzhen, China Researchers from Salk Institute for Biological ... time evaluated the safety and reliability of the ... a new method, TALEN-HDAdV, which could significantly increased ... (hiPSC). This study published online in Cell ... for stem cell-based gene therapy. , The combination ...
Breaking Biology News(10 mins):Belize's lobster, conch, and fish populations rebuild in no-take zones 2New professorship in tissue engineering links molecular engineering, marine biology 2A new genome editing method brings the possibility of gene therapies closer to reality 2
... a common goal: making the invisible visible. Yet artistically ... careers. A new four-year, $1.2 million program led by ... and physics of color into a series of summer ... art-interested students to enter careers in science. , ...
... be heading into the rainforests of Nicaragua to help an endangered ... crops are being threatened by the animals. The animals were ... just two years ago when the MSU team discovered them still ... up of still and video cameras in order to "capture" the ...
... New Rochelle, NY, November 15, 2012Epstein-Barr virus (EBV) is ... serious disease in liver transplant recipients. Molecular tests that ... have value for diagnosing active infection. The benefits of ... EBV-infected transplant patients are evaluated in an article published ...
Cached Biology News:New programs draws young artists into science 2Collaring tapirs to help them survive 2
(Date:7/11/2014)... Cayenne Medical, Inc., a privately held ... tissue reconstruction segment, announced the worldwide launch of ... the shoulder and extremities. The SureLock System provides ... method. The unique delivery eliminates manual tensioning that ... anchor displacement (also commonly know as anchor “creep”). ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Deep ... Century Medicine Forum on ‘Commercialising Longevity Research’ and ... entrepreneurs to the London Bioscience Innovation Center for ... Foundation and Aging Analytics Ltd. The meeting highlighted ... in translational research for age-related disease, as well ...
(Date:7/10/2014)... Francisco, CA (PRWEB) July 10, 2014 ... family closer together. The Archer Family purchased a ... contained within DNA, but ended up with a lifetime ... discoveries contained within the family members’ DNA genuinely brought ... had decided to search deeper into genetic history and ...
(Date:7/10/2014)... EvoDerma ’s NOOME Anti-Aging Motion has ... now comes with a second treatment cup to help prevent ... new cup is thinner on the edges for a softer ... thin and uneven surfaces on the face, neck and décolleté. ... treatment on areas such as the cheeks and neck, or ...
Breaking Biology Technology:Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 2Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2
... 23, 2010Scientists at the Gladstone Institute of Neurological Disease ... in Alzheimer,s disease (AD) and other neurodegenerative diseases. The ... "We examined a protein called tau that ... Gan, PhD, senior author on the study. "Tau forms ...
... LONDON and SAN FRANCISCO, September 22, 2010 ... ... ... Results are keyed by longUrl, so we need to grab the first one. for (var r in ...
... SINGAPORE, Sept. 23 S*BIO Pte Ltd today ... that will support the continued development of S*BIO,s ... commitment reflects the international investment community,s strong interest ... through clinical development and the quality of our ...
Cached Biology Technology:Gladstone scientists identify strategy to reduce toxic proteins associated with Alzheimer's disease 2Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 2Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 3Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 4Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 5Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 6Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 7Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 8Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 9Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 10S*BIO Announces US$5 Million Investment by Mitsui Ventures 2S*BIO Announces US$5 Million Investment by Mitsui Ventures 3
... is one of several isoprostanes produced from arachidonic ... renal vasoconstrictor in the rat. 8-iso PGE2 inhibits ... of 0.5 and 5 µM, respectively. When infused ... a concentration of 4 mg/kg/min, 8-iso PGE2 decreases ...
DNA Polymerase I Large (Klenow) Fragment is a DNA polymerase that lacks the 5'Cut Site3' exodeoxyribonuclease activity of intact DNA Polymerase I but it contains the 3'Cut Site5' exodeoxyribonuclease...
... offers both high-speed and high-volume versatility ... rotor options, making it ideal for a ... Versatility , High-speed (up to 15,000 ... high-volume (4 x 250mL) swinging bucket rotors ...
Guanylate Kinase Purified Anti-Mouse, Anti-Rat clone 28, Isotype Mouse IgG 1 , 50 µg Consult technical datasheet for details....
Biology Products: