Navigation Links
Mouse brain seen in sharpest detail ever
Date:10/25/2010

DURHAM, N.C. The most detailed magnetic resonance images ever obtained of a mammalian brain are now available to researchers in a free, online atlas of an ultra-high-resolution mouse brain, thanks to work at the Duke Center for In Vivo Microscopy.

In a typical clinical MRI scan, each pixel in the image represents a cube of tissue, called a voxel, which is typically 1x1x3 millimeters. "The atlas images, however, are more than 300,000 times higher resolution than an MRI scan, with voxels that are 20 micrometers on a side," said G. Allan Johnson, Ph.D., who heads the Duke Center for In Vivo Microscopy and is Charles E. Putman Distinguished Professor of Radiology.

The interactive images in the atlas will allow researchers worldwide to evaluate the brain from all angles and assess and share their mouse studies against this reference brain in genetics, toxicology and drug discovery.

The brain atlas' detail reaches a resolution of 21 microns. A micron is a millionth of a meter, or 0.00003937 of an inch.

An article detailing the creation of the atlas was published as the cover story in the November issue of NeuroImage journal.

The atlas used three different magnetic resonance microscopy protocols of the intact brain followed by conventional histology to highlight different structures in the reference brain. The brains were scanned using an MR system operating at a magnetic field more than 6 times higher than is routinely used in the clinic. The images were acquired on fixed tissues, with the brain in the cranium to avoid the distortion that occurs when tissues are thinly sliced for conventional histology.

The new Waxholm Space brain can be digitally sliced from any plane or angle, so that researchers can precisely visualize any regions in the brain, along any axis without loss of spatial resolution. (Waxholm is the Swedish town where the early concepts gelled for this atlas.)

"Researchers can take the reference brain apart and put it back together, because we have the 3-D data set intact, and any section will have the same excellent resolution," said Johnson, who is also a professor of biomedical engineering and physics at Duke. "It eliminates the Humpty Dumpty problem that researchers used to face when they made 3-D measurements of brain structures."

For example, a geneticist might want to alter a mouse's genotype in an experiment and learn what happens when the animal becomes highly responsive to fear challenges. "It would be interesting to see if the amygdala, (a brain center related to emotional arousal), is really smaller or larger in the animal," Johnson said. "However, if you do conventional histology, the animal brain shrinks when it is dried or prepared in alcohol, sometimes by about 40 percent. Because of variability, that would make it challenging to measure. This atlas provides a reference to measure against."

The team was also able to digitally segment 37 unique brain structures using the three different data acquisition strategies.

Scientists obtained images of brains from eight mice of the most frequently used strain of laboratory mice (C57BL), aged 66-78 days old. They registered the images together and created both an average and a probabilistic brain for reference. The average and probabilistic brains provide quantitative measure of variability. "It was truly remarkable how alike these structures were from brain to brain," he said.

All of the data is available on the web: www.civm.duhs.duke.edu/neuro201001.

As new data is gathered from other sources, researchers will be able to register it to the same coordinate system, which will promote data sharing, Johnson said. For example the Duke group has recently added data also at the highest resolution yet attained that allows definition of fiber tracts connecting different parts of the brain. Investigators at the Allen Brain Institute are now using the MR data to provide 3-D location for their extensive gene expression studies (http://mouse.brain-map.org).


'/>"/>

Contact: Mary Jane Gore
mary.gore@duke.edu
919-660-1309
Duke University Medical Center
Source:Eurekalert  

Related biology news :

1. Exposure to low doses of BPA alters gene expression in the fetal mouse ovary
2. Scientists reprogram mouse fat cells into clinically useful stem cells
3. Mouse stem cell study offers new insights into body fat distribution
4. Scientists create 3-D models of whole mouse organs
5. UCSF transgenic mouse mimics Parkinsons earliest symptoms
6. Intentional variation increases result validity in mouse testing
7. Mouse work: New insights on a fundamental DNA repair mechanism
8. Stem cells restore sight in mouse model of retinitis pigmentosa
9. Penn biologists determine microRNA activity is suppressed in mouse ovum
10. CCNY biologists identify new spiny pocket mouse species
11. Chloride increases response to pheromones and odors in mouse sensory neurons
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Mouse brain seen in sharpest detail ever
(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
(Date:4/3/2017)... , April 3, 2017  Data captured ... engineering platform, detected a statistically significant association ... prior to treatment and objective response of ... potential to predict whether cancer patients will ... treatment, as well as to improve both pre-infusion ...
(Date:3/29/2017)... 29, 2017  higi, the health IT company that ... North America , today announced a Series B ... of EveryMove. The new investment and acquisition accelerates higi,s ... to transform population health activities through the collection and ... higi collects and secures data today on behalf ...
Breaking Biology News(10 mins):
(Date:6/16/2017)... ... June 16, 2017 , ... Cognition Corporation , ... two more sessions of its “From the Helm” Webinar Series. , The ... online templates for design control exercises. Led by David Cronin, Cognition’s CEO, the ...
(Date:6/15/2017)... ... 15, 2017 , ... Cybrexa Therapeutics, a start-up cancer therapeutics ... in the amount of $6 million. An investment vehicle affiliated with HighCape Partners, ... The Series B funding will enable Cybrexa to complete the build-out of its ...
(Date:6/15/2017)... ... 15, 2017 , ... Adam Equipment, a leading provider of ... lab workstation. The guide outlines the procedure in four simple steps, using comprehensive ... anti-vibration table, OIML/ASTM certified weights, and Adam DU specialized software. , A lab ...
(Date:6/14/2017)... USA – , ... ... June 14, 2017 -- Diagenode, a leading global provider ... has licensed a new technology specific for ChIP-sequencing from ... immunoprecipitation followed by sequencing (ChIP-seq) allows the study of ...
Breaking Biology Technology: