Navigation Links
Molecular technique advances soybean rust resistance research

A new tool is available to select for soybean rust resistance in breeding populations, said Glen Hartman, University of Illinois professor of crop sciences and USDA-ARS scientist. Hartman and his team of researchers successfully used quantitative polymerase chain reaction (Q-PCR) assays to assess fungal DNA in soybean leaf tissue to quantify the level of resistance in individual plants with resistance to soybean rust.

"This is not a new technique," Hartman said. "But it is a new tool for use in soybean rust resistance breeding, which has typically used phenotyping or visual assessment to measure resistance. We discovered that we can perform more precise and quicker assessments using this molecular technique."

Visual assessment is subject to interpretation and is not an exact science, Hartman said. However, Q-PCR allows for exact enumeration of fungal DNA in the tissue. This is particularly helpful when plants show similar visual symptoms, but colonization levels vary based on fungal DNA levels.

"The eye can easily tell us if it's a plus or minus for qualitative resistance, but Q-PCR tells us the quantitative resistance or the gray that lies between the plus and minus," Hartman added.

Often qualitative resistance doesn't last as long as quantitative resistance because it involves a single gene. Pathogens can overcome a single gene more easily, putting soybean breeders right back to where they started with a susceptible reaction, he said.

"In quantitative resistance where multiple genes are working together to form resistance, breeders have to distinguish the gray area between susceptible and resistant," Hartman said. "It takes a lot to do that visually with your eye. You can look at samples under a microscope and take multiple measurements But, it's hard and time consuming, particularly when you are working with breeding populations and hundreds of samples."

Hartman said this technique will be useful for plant breeders trying to breed soybeans for resistance to soybean rust.

"We believe Q-PCR will save time and be more precise," he said. "The precision part is very important. The more precise you can be, knowing exactly what the line is reacting to, will lead to more precise mapping of the quantitative resistance genes."

The mapping of this particular quantitative resistance is very important to breeders selecting for rust resistance, Hartman said.

"It's a numbers game," he said. "In developing soybean cultivars, a large number of lines need to be evaluated so many inferior lines have to be discarded. In terms of breeding for soybean rust resistance, this technique can help determine which lines are more resistant to rust when it comes to the gray areas or quantitative resistance."


Contact: Jennifer Shike
University of Illinois College of Agricultural, Consumer and Environmental Sciences

Related biology news :

1. Several baffling puzzles in protein molecular structure solved with new method
2. Molecular movements of neural transporters unveiled
3. Low-cost molecular medicine should be included in modern eye physicians tool kit
4. Molecular messages from the antennae
5. Chemical engineers at UCSB design molecular probe to study disease
6. Molecular muscle: Small parts of a big protein play key roles in building tissues
7. Salk scientists crack molecular code regulating neuronal excitability
8. New molecular robot can be programmed to follow instructions
9. Molecular mechanism contributing to neuronal circuit formation found
10. Combined molecular study techniques reveal more about DNA proteins
11. Fingerprints match molecular simulations with reality
Post Your Comments:
Related Image:
Molecular technique advances soybean rust resistance research
(Date:4/11/2017)... GARDENS, Fla. , April 11, 2017 /PRNewswire/ ... management and secure authentication solutions, today announced that ... by Intelligence Advanced Research Projects Activity (IARPA) to ... IARPA,s Thor program. "Innovation has been ... and IARPA,s Thor program will allow us to ...
(Date:4/5/2017)...  The Allen Institute for Cell Science today announces ... portal and dynamic digital window into the human cell. ... application of deep learning to create predictive models of ... a growing suite of powerful tools. The Allen Cell ... publicly available resources created and shared by the Allen ...
(Date:3/30/2017)...  On April 6-7, 2017, will host the ... hackathon at Microsoft,s headquarters in Redmond, Washington ... developing health and wellness apps that provide a unique, ... is the first hackathon for personal genomics and ... in the genomics, tech and health industries are sending ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... 2017 , ... At its national board meeting in North ... the co-founder, CEO and chief research scientist of Minnesota-based Advanced Space Technology and ... ARCS Alumni Hall of Fame . ASTER Labs is a technology development ...
(Date:10/11/2017)... Oct. 11, 2017  VMS BioMarketing, a leading provider of ... oncology Clinical Nurse Educator (CNE) network, which will launch this ... communication among health care professionals to enhance the patient care ... staff, and other health care professionals to help women who ... ...
(Date:10/10/2017)... SAN DIEGO, CALIF. (PRWEB) , ... October 10, 2017 , ... ... website as part of its corporate rebranding initiative announced today. The bold new ... broaden its reach, as the company moves into a significant growth period. , It ...
(Date:10/10/2017)... , Oct. 10, 2017 SomaGenics announced ... the NIH to develop RealSeq®-SC (Single Cell), expected to ... profiling small RNAs (including microRNAs) from single cells using ... highlights the need to accelerate development of approaches to ... "New techniques for measuring levels of ...
Breaking Biology Technology: