Navigation Links
Molecular technique advances soybean rust resistance research
Date:5/16/2011

A new tool is available to select for soybean rust resistance in breeding populations, said Glen Hartman, University of Illinois professor of crop sciences and USDA-ARS scientist. Hartman and his team of researchers successfully used quantitative polymerase chain reaction (Q-PCR) assays to assess fungal DNA in soybean leaf tissue to quantify the level of resistance in individual plants with resistance to soybean rust.

"This is not a new technique," Hartman said. "But it is a new tool for use in soybean rust resistance breeding, which has typically used phenotyping or visual assessment to measure resistance. We discovered that we can perform more precise and quicker assessments using this molecular technique."

Visual assessment is subject to interpretation and is not an exact science, Hartman said. However, Q-PCR allows for exact enumeration of fungal DNA in the tissue. This is particularly helpful when plants show similar visual symptoms, but colonization levels vary based on fungal DNA levels.

"The eye can easily tell us if it's a plus or minus for qualitative resistance, but Q-PCR tells us the quantitative resistance or the gray that lies between the plus and minus," Hartman added.

Often qualitative resistance doesn't last as long as quantitative resistance because it involves a single gene. Pathogens can overcome a single gene more easily, putting soybean breeders right back to where they started with a susceptible reaction, he said.

"In quantitative resistance where multiple genes are working together to form resistance, breeders have to distinguish the gray area between susceptible and resistant," Hartman said. "It takes a lot to do that visually with your eye. You can look at samples under a microscope and take multiple measurements But, it's hard and time consuming, particularly when you are working with breeding populations and hundreds of samples."

Hartman said this technique will be useful for plant breeders trying to breed soybeans for resistance to soybean rust.

"We believe Q-PCR will save time and be more precise," he said. "The precision part is very important. The more precise you can be, knowing exactly what the line is reacting to, will lead to more precise mapping of the quantitative resistance genes."

The mapping of this particular quantitative resistance is very important to breeders selecting for rust resistance, Hartman said.

"It's a numbers game," he said. "In developing soybean cultivars, a large number of lines need to be evaluated so many inferior lines have to be discarded. In terms of breeding for soybean rust resistance, this technique can help determine which lines are more resistant to rust when it comes to the gray areas or quantitative resistance."


'/>"/>

Contact: Jennifer Shike
jshike@illinois.edu
217-244-0888
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert  

Related biology news :

1. Several baffling puzzles in protein molecular structure solved with new method
2. Molecular movements of neural transporters unveiled
3. Low-cost molecular medicine should be included in modern eye physicians tool kit
4. Molecular messages from the antennae
5. Chemical engineers at UCSB design molecular probe to study disease
6. Molecular muscle: Small parts of a big protein play key roles in building tissues
7. Salk scientists crack molecular code regulating neuronal excitability
8. New molecular robot can be programmed to follow instructions
9. Molecular mechanism contributing to neuronal circuit formation found
10. Combined molecular study techniques reveal more about DNA proteins
11. Fingerprints match molecular simulations with reality
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Molecular technique advances soybean rust resistance research
(Date:8/15/2017)... LLC , a medical device company focused on improving the safety ... ISO 13485 Certification, the global standard for medical device quality management ... ... for the early detection of IV infiltrations. ... "This is an important milestone for ivWatch, as it validates our ...
(Date:5/23/2017)... Italy , May 23, 2017  Hunova, the first robotic gym ... trunk, has been officially launched in Genoa, Italy . ... Europe and the USA . The technology ... on the market by the IIT spin-off Movendo Technology thanks to a ... the Multimedia News Release, please click: ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
Breaking Biology News(10 mins):
(Date:9/14/2017)... ... , ... AIM Global, the worldwide standards agency for AIDC and barcode technology ... Study Competition for AIDC. The award is given to those in the industry ... adoption of automated data collection systems further. The TRACTUS platform, by Matrix, is ...
(Date:9/13/2017)... MA (PRWEB) , ... September 13, 2017 , ... ... panel and networking event on Thursday, September 21st from 6 to 8:30pm ... the topic of “Proactive Patient Enrollment Strategies for Clinical Operations Professionals.” From tracking enrollment ...
(Date:9/12/2017)... NC (PRWEB) , ... September ... ... Inc. ( http://www.globalspecimensolutions.com ) has announced the release of their latest product ... workflows and storage requirements. LabCODE™ offers pre-built capabilities for Biobanking, Pharmacokinetics, ...
(Date:9/12/2017)... ... September 12, 2017 , ... ... pleased to announce that Jeremy Nicholson, Head of the Department of Surgery and ... deliver the 2018 Wallace H. Coulter Lecture. His presentation, “Analytical Science in ...
Breaking Biology Technology: