Navigation Links
Molecular technique advances soybean rust resistance research

A new tool is available to select for soybean rust resistance in breeding populations, said Glen Hartman, University of Illinois professor of crop sciences and USDA-ARS scientist. Hartman and his team of researchers successfully used quantitative polymerase chain reaction (Q-PCR) assays to assess fungal DNA in soybean leaf tissue to quantify the level of resistance in individual plants with resistance to soybean rust.

"This is not a new technique," Hartman said. "But it is a new tool for use in soybean rust resistance breeding, which has typically used phenotyping or visual assessment to measure resistance. We discovered that we can perform more precise and quicker assessments using this molecular technique."

Visual assessment is subject to interpretation and is not an exact science, Hartman said. However, Q-PCR allows for exact enumeration of fungal DNA in the tissue. This is particularly helpful when plants show similar visual symptoms, but colonization levels vary based on fungal DNA levels.

"The eye can easily tell us if it's a plus or minus for qualitative resistance, but Q-PCR tells us the quantitative resistance or the gray that lies between the plus and minus," Hartman added.

Often qualitative resistance doesn't last as long as quantitative resistance because it involves a single gene. Pathogens can overcome a single gene more easily, putting soybean breeders right back to where they started with a susceptible reaction, he said.

"In quantitative resistance where multiple genes are working together to form resistance, breeders have to distinguish the gray area between susceptible and resistant," Hartman said. "It takes a lot to do that visually with your eye. You can look at samples under a microscope and take multiple measurements But, it's hard and time consuming, particularly when you are working with breeding populations and hundreds of samples."

Hartman said this technique will be useful for plant breeders trying to breed soybeans for resistance to soybean rust.

"We believe Q-PCR will save time and be more precise," he said. "The precision part is very important. The more precise you can be, knowing exactly what the line is reacting to, will lead to more precise mapping of the quantitative resistance genes."

The mapping of this particular quantitative resistance is very important to breeders selecting for rust resistance, Hartman said.

"It's a numbers game," he said. "In developing soybean cultivars, a large number of lines need to be evaluated so many inferior lines have to be discarded. In terms of breeding for soybean rust resistance, this technique can help determine which lines are more resistant to rust when it comes to the gray areas or quantitative resistance."


Contact: Jennifer Shike
University of Illinois College of Agricultural, Consumer and Environmental Sciences

Related biology news :

1. Several baffling puzzles in protein molecular structure solved with new method
2. Molecular movements of neural transporters unveiled
3. Low-cost molecular medicine should be included in modern eye physicians tool kit
4. Molecular messages from the antennae
5. Chemical engineers at UCSB design molecular probe to study disease
6. Molecular muscle: Small parts of a big protein play key roles in building tissues
7. Salk scientists crack molecular code regulating neuronal excitability
8. New molecular robot can be programmed to follow instructions
9. Molecular mechanism contributing to neuronal circuit formation found
10. Combined molecular study techniques reveal more about DNA proteins
11. Fingerprints match molecular simulations with reality
Post Your Comments:
Related Image:
Molecular technique advances soybean rust resistance research
(Date:5/24/2016)... , May 24, 2016 Ampronix facilitates superior patient care by providing ...  3D medical LCD display is the latest premium product recently added to the range ... ... ... Sony 3d Imaging- LCD Medical Display- Ampronix News ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
(Date:4/19/2016)... The new GEZE SecuLogic access ... "all-in-one" system solution for all door components. It can ... door interface with integration authorization management system, and thus ... minimal dimensions of the access control and the optimum ... offer considerable freedom of design with regard to the ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... A person commits a crime, and the detective ... the criminal down. An outbreak of foodborne illness ... (FDA) uses DNA evidence to track down the bacteria that ... It,s not. The FDA has increasingly used a complex, cutting-edge ... illnesses. Put as simply as possible, whole genome sequencing is ...
(Date:6/23/2016)... FRANCISCO , June 23, 2016   EpiBiome ... has secured $1 million in debt financing from Silicon ... ramp up automation and to advance its drug development ... its new facility. "SVB has been an ... beyond the services a traditional bank would provide," said ...
(Date:6/23/2016)... 23, 2016  Blueprint Bio, a company dedicated to ... medical community, has closed its Series A funding round, ... "We have received a commitment from Forentis ... need to meet our current goals," stated Matthew ... runway to complete validation on the current projects in ...
(Date:6/23/2016)... ... June 23, 2016 , ... Velocity Products, a division of ... and optimized exclusively for Okuma CNC machining centers at The International Manufacturing Technology ... among several companies with expertise in toolholding, cutting tools, machining dynamics and distribution, ...
Breaking Biology Technology: