Navigation Links
Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
Date:4/30/2012

nerve growth factor, a small protein, is introduced into a PC12 cell, the cell begins to send out neurites resembling the projections from nerve cell bodies. Although originally derived from a tumor of the rat's adrenal gland, PC12 has become, rather counterintuitively, a valuable model of how nerve cells differentiate from their unspecialized progenitors.

Berkeley Lab postdoctoral fellow Liang Chen began the current experiments by introducing nerve growth factor to groups of PC12 cells to induce them to differentiate; one group of cells was left untreated as a control. The cells were cultured on gold-coated slides in chambers maintained at body temperature in a humidified environment and supplied with nutrients. Individual cells of a group were positioned under the infrared beam at the beamline 1.4.3 endstation.

FTIR spectra were collected before and after the nerve growth factor was introduced. After stimulation, the spectra were taken first at short intervals, from two to sixty minutes apart. Additional spectra were collected of cells in other groups on the third, fifth, and seventh day of continued stimulation.

The first day's spectra revealed spikes in phosphorylation activity within minutes after the addition of the nerve growth factor, in concert with changes in the ratios of such important chemical contents of the cell as proteins, carbohydrates, and lipids. Phosphorylation subsequently waned, then picked up again in another burst of activity on Day 3, just as the cells began to extend neurites.

By comparing results with quantum chemistry simulations by Berkeley Lab's Zhao Hao predicting what should be observed from first principles as well as with results from partial studies using other methods, the researchers confirmed the monitoring of phosphorylation phases, their timing, and their target proteins, along with associated changes in other substances in the cell.

A new technique takes off'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. PNAS: Precise molecular surgery in the plant genome
2. 5th Annual Advances in Biomolecular Engineering Symposium
3. 2012 Forecast for US Molecular Diagnostics Market Now Available From Global Information Inc.
4. Beyond the microscope: Identifying specific cancers using molecular analysis
5. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
6. The molecular basis of touch sensation
7. MDC researchers reveal molecular mechanism underlying severe anomalies of the forebrain
8. Evolution of complexity recreated using molecular time travel
9. Study uncovers a molecular maturation clock that modulates branching architecture in tomato plants
10. AACR & IASLC to host molecular origins of lung cancer conference
11. Controlled disorder -- scientists find way to form random molecular patterns
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
(Date:10/16/2014)... new synthesis of recent research findings to inform their ... two states. , The Ecology and Management of ... Synthesis of the Relevant Biophysical Science and Implications for ... the U.S. Forest Service,s Pacific Northwest Research Station, is ... a synthesis of the large body of scientific information ...
(Date:10/16/2014)... , October 16, 2014 NXT-ID, Inc. ... the "Company"), a biometric authentication company focused on the ... leading edge technologies including Wocket™, the Smart Wallet, at ... Conferenece. FiRe is a leading global conference on the ... Hosted by Mark Anderson , founder and publisher ...
(Date:10/15/2014)... The Ebola virus is spreading rapidly and to an ... experienced in the past and the virus shows a ... been recorded before. For this reason, the German National ... of Science and Engineering, and the Union of the ... statement on the Ebola epidemic today. , In the ...
Breaking Biology News(10 mins):New report synthesizes best available science on management of moist mixed-conifer forests 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 3Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5
... have discovered a type of cell that may contribute to ... sclerosis and rheumatoid arthritis strike women more frequently than men. ... which bind to and attack the body,s own tissue. The ... journal Blood , that they found higher levels of ...
... available in Spanish . U.S. Department ... food-aid product called Instant Corn Soy Blend that supplements ... led by food technologist Charles Onwulata at the Agricultural ... at the agency,s Eastern Regional Research Center (ERRC) in ...
... Researchers demonstrate for the first time that programmed cell ... themselves - is involved in mandibular regression in termites. ... to pay for the formation of termites, defensive organs, ... University of Toyama, and Shinichi Yoda from the University ...
Cached Biology News:B-cell discovery suggests why women suffer more autoimmune disease 2Researchers develop fully cooked food-aid product 2Cells die so defensive organs can live 2
(Date:10/22/2014)... The Americas Inorganic Refrigerants Market report ... Americas with analysis and forecast of revenue. , ... Refrigerants Market report, to get an idea of ... glimpse of the segmentation in the Americas inorganic ... and figures. , http://www.micromarketmonitor.com/market/americas-inorganic-refrigerants-4191654241.html , ...
(Date:10/22/2014)... San Diego, CA (PRWEB) October 22, 2014 ... laboratory balances from Sartorius, A & D Weighing, ... Sartorius CPA Semi-Micro Balance . The Sartorius ... looking for an affordable high-quality, precise, and user-friendly ... in manufacturing of laboratory equipment, their laboratory balances ...
(Date:10/22/2014)... , Oct. 22, 2014 /PRNewswire/ - iCo Therapeutics ("iCo" ... announced next steps for its Oral Amphotericin B ... its in vitro work involving samples ... now plans to complete pre clinical studies and ... Phase 1A clinical trial, utilizing approximately $700,000 of ...
(Date:10/20/2014)... 2014 Mapp Biopharmaceutical,s valiant effort to ... to fight the Ebola outbreak will make the ... production of pharmaceuticals can be, according to Kalorama ... while some may be taken aback by the ... industry knowledge are well aware of the complex ...
Breaking Biology Technology:The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 2The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 3Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 3iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 3
... University nanoscientist Chad A. Mirkin has mass-produced the 2008 Summer ... only one square centimeter of space. , Mirkin and ... gold circuit using a new printing technique, called Polymer Pen ... using only one device. , The PPL technique, to ...
... SOUTH EASTON, Mass., Aug. 14 Pressure,BioSciences, Inc. (Nasdaq: ... total revenue for the second quarter of 2008 was ... prior year. Revenue from the sale,of PCT products and ... 2008 as compared to $136,355 for the prior year ...
... Awards recognize innovative biopharmaceutical drugs and devices that have ... a deep impact on the quality of human life -, ... CVTX ) today announced that its chronic angina therapy,Ranexa(R) ... prestigious Prix Galien USA 2008 award which honors the,best pharmaceutical ...
Cached Biology Technology:Northwestern chemists take gold, mass-produce Beijing Olympic logo 2Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 2Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 3Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 4Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 5Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 6Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 7Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 8Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 9CV Therapeutics Announces Ranexa(R) Named Finalist for Prix Galien USA 2008 Award 2CV Therapeutics Announces Ranexa(R) Named Finalist for Prix Galien USA 2008 Award 3
... Team The Schleicher & Schuell ... work center dedicated to custom protein ... facilitys experienced project teams, comprised of ... professionals, work closely with collaborators every ...
... Western blotting is a commonly used technique ... protein samples are electrophoresed on SDS-PAGE and ... or nylon, where they are probed with ... which allow reuse of Southern and Northern ...
... Peptide microarrays containing peptidomimetics built on ... on-chip synthesis platform. These microarrays are ... Custom Peptide Microarray Service. ... is completely customer specified. Thousands of ...
... time, you can identify and characterize high-value, ... interferences. The Agilent Multiple Affinity Removal System ... proteins from human serum, plasma, CSF samples ... With this revolutionary system, you can: ...
Biology Products: