Navigation Links
Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
Date:4/30/2012

Knowing how a living cell works means knowing how the chemistry inside the cell changes as the functions of the cell change. Protein phosphorylation, for example, controls everything from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death (apoptosis), in cells from bacteria to humans. It's a chemical process that has long been intensively studied, not least in hopes of treating or eliminating a wide range of diseases. But until now the close-up view watching phosphorylation at work on the molecular level as individual cells change over time has been impossible without damaging the cells or interfering with the very processes that are being examined.

"To look into phosphorylation, researchers have labeled specific phosphorylated proteins with antibodies that carry fluorescent dyes," says Hoi-Ying Holman of the U.S. Department of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab). "That gives you a great image, but you have to know exactly what to label before you can even begin."

Holman and her coworkers worked with colleagues from the San Diego and Berkeley campuses of the University of California to develop a new technique for monitoring protein phosphorylation inside single living cells, tracking them over a week's time as they underwent a series of major changes.

"Now we can follow cellular chemical changes without preconceived notions of what they might be," says Holman, a pioneer in infrared (IR) studies of living cells who is director of the Berkeley Synchrotron Infrared Structural Biology program at Berkeley Lab's Advanced Light Source (ALS) and head of the Chemical Ecology Research group in the Earth Sciences Division. "We've monitored unlabeled living cells by studying the nonperturbing absorption of a wide spectrum of bright synchrotron infrared radiation from the ALS."

The researchers report their results in the journal Analytical Chemistry.


'/>"/>
Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. PNAS: Precise molecular surgery in the plant genome
2. 5th Annual Advances in Biomolecular Engineering Symposium
3. 2012 Forecast for US Molecular Diagnostics Market Now Available From Global Information Inc.
4. Beyond the microscope: Identifying specific cancers using molecular analysis
5. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
6. The molecular basis of touch sensation
7. MDC researchers reveal molecular mechanism underlying severe anomalies of the forebrain
8. Evolution of complexity recreated using molecular time travel
9. Study uncovers a molecular maturation clock that modulates branching architecture in tomato plants
10. AACR & IASLC to host molecular origins of lung cancer conference
11. Controlled disorder -- scientists find way to form random molecular patterns
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
(Date:8/18/2015)... , Aug. 18, 2015  Navitas today ... contributor to the Submission Content Management webinar ... a non-profit forum. Dedicated to the Implementation of ... neutral platform for industry, vendors, health authorities, ... and work towards a standard method of ...
(Date:8/17/2015)... 17, 2015  NXT-ID, Inc. (NASDAQ: NXTD ) ... on the growing mobile commerce market and creator of ... for Q4 2015. The new marketing campaign is being ... Amplitude Marketing Group ( http://amplitudemarketing.com/ ) and TPMG ( ... leader in retail driven marketing and brand awareness and ...
(Date:8/12/2015)... August 12, 2015 As the ... payment innovation and advanced biometrics technology is accelerating beyond ... consumers rely on using their credit cards, the digital ... future for payment services led by companies in the ... :  Google, Inc. (NASDAQ: GOOG ), Apple Inc. ...
Breaking Biology News(10 mins):Navitas, a Key Contributor in IRISS Forum Webinar 2Navitas, a Key Contributor in IRISS Forum Webinar 3NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 2NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 2Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 4Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 5Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 6
... the traditional summer treat associated with Wimbledon could ... Scientists at the University of Warwick have been ... cardiovascular health, particularly around how they prevent the ... Paul Thornalley from Warwick Medical School heads the ...
... and significance of the biological clocks in polar planktonic ... entitled PolarTime starting July 1st, 2012. It is one ... Association. PolarTime, coordinated by the Alfred Wegener Institute for ... to five years with approximately three million euros from ...
... been made in most developing countries to meet the ... who suffer from hunger by 2015 compared with 1990 ... children,s height and weight, published today in the ... five has improved overall since 1985, one in five ...
Cached Biology News:Just a question of time? 2Just a question of time? 3Developing world has less than 5 percent chance of meeting UN child hunger target, study estimates 2Developing world has less than 5 percent chance of meeting UN child hunger target, study estimates 3
(Date:8/26/2015)... N.J. , Aug. 26, 2015  Roka Bioscience, ... focused on providing advanced testing solutions for the detection ... CEO will present at the Sidoti & Company Emerging ... conference will be held at the New York Marriott ... presentation will be available through the investor relations section ...
(Date:8/26/2015)... FRANCISCO , Aug. 26, 2015  Platform ... that it has received a $1.5M Phase II ... (NIH) to further develop its therapeutic agent to ... clinical need in end stage renal disease (ESRD) ... the National Institute of Diabetes and Digestive and ...
(Date:8/26/2015)... HONG KONG , Aug. 26, 2015 ... "Company"), China,s leading provider of ... and stem cell storage services, today announced that the ... Holdings Limited ("MO2"), an entity wholly owned by Mr. ... the Company, has acquired the outstanding shares (the "Shares") ...
(Date:8/26/2015)... ... August 26, 2015 , ... PRC Clinical, a Clinical Trial ... with local sponsors in southern California. The Clinical Trial Management Expert CRO seeks to ... regulatory pathways., , Stem Cell Meeting on the Mesa ...
Breaking Biology Technology:Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 2Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 3China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 2China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 3PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 2PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 3
... 26 in Kansas City. , ... Rockville, MD (PRWEB) ... Scientific Meeting (ASM) will be held in Kansas City, Mo., September ... the USP experts responsible for establishing internationally recognized standards for prescription ...
... Oncology Specialty, LP, the,state-of-the-art pharmaceutical distribution center of ... from the National,Safety Council for its outstanding safety ... who produce the "best of the best",in safety ... report,days away from work resulting from workplace injuries ...
... 3 Inverness Medical,Innovations, Inc. (Amex: IMA ... products for the consumer and professional markets, today,announced ... and Jon Russell, Vice President, Finance, are scheduled ... Conference on,Tuesday, September 9th, 2008 in New York. ...
Cached Biology Technology:USP's 2008 Annual Scientific Meeting to Convene Scientific Experts from Around the World 2USP's 2008 Annual Scientific Meeting to Convene Scientific Experts from Around the World 3US Oncology Pharmaceutical Distribution Service Wins National Safety Council Award 2
...
Hygromycin resistance gene (hph), provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
... 30 isolations. The kit provides a ... apoptotic cells from normal c lture using ... is a Ca2+-dependent phospholipid binding protein with ... Cell Isolation Kit provides a simple and ...
... Linkage: This CLS number is ... to easily match Cornings product ... yet, please order under the ... contact customer service for assistance. ...
Biology Products: