Navigation Links
Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
Date:4/30/2012

Knowing how a living cell works means knowing how the chemistry inside the cell changes as the functions of the cell change. Protein phosphorylation, for example, controls everything from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death (apoptosis), in cells from bacteria to humans. It's a chemical process that has long been intensively studied, not least in hopes of treating or eliminating a wide range of diseases. But until now the close-up view watching phosphorylation at work on the molecular level as individual cells change over time has been impossible without damaging the cells or interfering with the very processes that are being examined.

"To look into phosphorylation, researchers have labeled specific phosphorylated proteins with antibodies that carry fluorescent dyes," says Hoi-Ying Holman of the U.S. Department of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab). "That gives you a great image, but you have to know exactly what to label before you can even begin."

Holman and her coworkers worked with colleagues from the San Diego and Berkeley campuses of the University of California to develop a new technique for monitoring protein phosphorylation inside single living cells, tracking them over a week's time as they underwent a series of major changes.

"Now we can follow cellular chemical changes without preconceived notions of what they might be," says Holman, a pioneer in infrared (IR) studies of living cells who is director of the Berkeley Synchrotron Infrared Structural Biology program at Berkeley Lab's Advanced Light Source (ALS) and head of the Chemical Ecology Research group in the Earth Sciences Division. "We've monitored unlabeled living cells by studying the nonperturbing absorption of a wide spectrum of bright synchrotron infrared radiation from the ALS."

The researchers report their results in the journal Analytical Chemistry.


'/>"/>
Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. PNAS: Precise molecular surgery in the plant genome
2. 5th Annual Advances in Biomolecular Engineering Symposium
3. 2012 Forecast for US Molecular Diagnostics Market Now Available From Global Information Inc.
4. Beyond the microscope: Identifying specific cancers using molecular analysis
5. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
6. The molecular basis of touch sensation
7. MDC researchers reveal molecular mechanism underlying severe anomalies of the forebrain
8. Evolution of complexity recreated using molecular time travel
9. Study uncovers a molecular maturation clock that modulates branching architecture in tomato plants
10. AACR & IASLC to host molecular origins of lung cancer conference
11. Controlled disorder -- scientists find way to form random molecular patterns
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
(Date:3/5/2015)... In Brazil , cloud computing is fast ... and 42 percent planning to invest in them by the ... the companies will be investing in these solutions for the ... the region are currently opting for the private cloud model, ... attention in the coming years. This trend is in line ...
(Date:3/3/2015)... , March 3, 2015 Cryoport, ... of advanced cryogenic logistics solutions for the ... stem cells, cell lines, clinical research organizations, ... today announced the expansion of its relationship ... ("Fred Hutch") Clinical Research Division with ...
(Date:3/2/2015)...  Businesses have a new option for premier ... Enterprises has announced the launch of a brand ... businesses to protect their customers, personal data, as ... Beconux is a biometric transaction processing system recently ... an ATM machine, the new system fuses multiple ...
Breaking Biology News(10 mins):Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 3Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 4Cryoport, Inc. Expands Support of Fred Hutchinson Cancer Research Center's Clinical Research Division 2Cryoport, Inc. Expands Support of Fred Hutchinson Cancer Research Center's Clinical Research Division 3Personal Data Protection Company Launches New Product 2
... Department of Energy,s (DOE) Savannah River National Laboratory (SRNL) ... on radiation ecology research, including projects in the region ... Power Plant 24 years ago. Researchers at IRL ... for studying the effects of radioactive contamination and methods ...
... a University of Pennsylvania method for evaluating a ... method has shown that 80 percent of dogs judged ... at risk for developing osteoarthritis and hip dysplasia, according ... traditional scoring of radiographs that certify dogs for breeding ...
... study from the Center for Interdisciplinary Chronobiological Research at ... between Light At Night (LAN) and cancer. This research ... the University of Haifa that also established the correlation. ... pollution, which the study has shown is a carcinogenic ...
Cached Biology News:SRNL, Chernobyl Laboratory collaborate on research initiatives 2Hip dysplasia susceptibility in dogs may be underreported, according to Penn Vet comparative study 2Hip dysplasia susceptibility in dogs may be underreported, according to Penn Vet comparative study 3Hip dysplasia susceptibility in dogs may be underreported, according to Penn Vet comparative study 4Connection between light at night (LAN) and cancer revealed in additional study 2
(Date:3/27/2015)... (PRWEB) March 27, 2015 Earlier this ... the promotion of hair regrowth in adult men and ... I-II or Norwood Hamilton classifications of IIa-V and Fitzpatrick ... industry plagued by products that over-promise and under-deliver, FDA ... renewing faith and confidence among more than 80 million ...
(Date:3/27/2015)... , 27. März 2015  Peter Walter ... Biomedical Science für seine bahnbrechende Arbeit ausgewählt, ... Zellkompartimenten transportiert werden, sowie für die Entschlüsselung ... Reaktion auf Stress nutzen, der mit der ... ist Professor für Biochemie an der University ...
(Date:3/27/2015)... , March 27, 2015   SurePure, ... liquid photopurification, announced today that the Company has ... the continued trading of SurePure ("SURP") securities on ... marketplace for entrepreneurial and development stage companies. ... introduced standards and eligibility requirements designed to improve ...
(Date:3/27/2015)... , March 27, 2015 Working in ... Hospital in London , Richmond Pharmacology is ... global Phase 3 study for an investigational RNAi therapeutic being ... disease affecting the nerves and heart. Read ... Based at St Georges University of ...
Breaking Biology Technology:Dr. Robert Leonard Applauds FDA Clearance of Capillus272Pro™ Hair Loss Laser Cap 2Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 2Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 3Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 4SurePure Completes Verification and Approved for Trading on the OTCQB Marketplace 2
... SAN DIEGO and EDISON, N.J., Nov. 4, 2010 ... Corporation (Nasdaq: THOR ), announced today the ... LLC, the global private equity firm, in an all ... a recognized worldwide leader in hemostasis management and Point-of-Care ...
... relatively advanced tools after their brains evolved a greater capacity ... why it took early humans almost two million years to ... Researchers used computer modelling and tiny sensors embedded in gloves ... in order to make two types of tools during the ...
... Corporation (Nasdaq: MATK ) announced today that ... / Mid Cap Conference being held on November 16-17, ... Officer is scheduled to present on Tuesday, November 16 ...  A live audio webcast of the presentation can be ...
Cached Biology Technology:International Technidyne Corporation Acquired by Warburg Pincus 2International Technidyne Corporation Acquired by Warburg Pincus 3Stone Age humans needed more brain power to make big leap in tool design 2Martek to Present at Upcoming Citi Small / Mid Cap Conference 2
... X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-galactopyranoside) is a ... identify recombinant plasmids. In ... and IPTG, lac ... blue, whereas recombinant colonies ...
... is an enhanced form of Tobacco Etch ... highly active, and significantly more stable than ... AcTEV Protease specifically recognizes a seven amino ... making it useful for removing affinity tags ...
versican (H-56)...
Vapor phase risers are available in various heights to accomodate a variety of inventory solutions. Risers are used to creat a false bottom in which the tanks platform rests and Liquid nitrogen is s...
Biology Products: