Navigation Links
Molecular movements of neural transporters unveiled
Date:4/25/2011

method does not simply generate information about the average movements of a collection of proteins. "Applying single-molecule imaging to the transporter gave us a unique view of the dynamics enabling the function of the transporters, because it allowed us to look at individual molecules and watch their movements in real time rather than time and ensemble averages," Dr. Blanchard says.

Last year, the researchers used smFRET to collect the first such single-molecule data for a membrane protein, and these results were also published in Nature. In their most recent experiments, they used the technique to monitor changes in LeuT conformation and dynamics by labeling moving parts of the protein with fluorescent dyes that emit distinct amounts of light when the distance separating them changes. As the transporter protein moves during function, time-dependent changes in distance between the fluorophores could be directly imaged to extract the first quantitative insights into the motions underpinning the transport mechanism.

Using powerful computational simulations, the researchers had predicted such movements through previous studies aimed at understanding how the transported molecule changes the conformation of LeuT. The new experiments demonstrated that alanine binding to LeuT increased the rate of the transporter's flickering between two conformations: facing outward, as if ready to accept substrates from outside the cell ("inward-closed"), and facing inward, as if releasing its contents into the cell ("inward-open"). How the presence of sodium affects the transporter's response to the binding of the transported substrate, alanine, was also revealed from these experiments: Sodium was essential for the alanine-enhanced dynamics. Surprisingly, alanine did not alter the total amount of time spent in either the open or closed state.

By contrast, the binding of sodium ions alone, without alanine, was found to decrease the transition rate between o
'/>"/>

Contact: Andrew Klein
ank2017@med.cornell.edu
212-821-0560
New York- Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. SNM releases new fact sheet on breast cancer and molecular imaging
2. Genetic Engineering & Biotechnology News reports on growing role of molecular diagnostics
3. A new journal where molecular biology meets clinical research
4. Scientists trace molecular origin of proportional development
5. New molecular insight into vertebrate brain development
6. Plants display molecular amnesia
7. Pitt, NETL researchers report molecular chain reaction thought to be impossible
8. Tracking the molecular pathway to mixed-lineage leukemia
9. Researchers compile molecular manual for 100s of inherited diseases
10. Biologists learn structure, mechanism of powerful molecular motor in virus
11. Phoenix conference highlights TGens and Scottsdale Healthcares contributions to molecular oncology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/28/2014)... of America (ESA) is pleased to announce the selection ... the Society. Honorary Membership acknowledges those who have served ... in the affairs of the Society that has reached ... by the ESA Governing Board and then voted on ... at the Awards Ceremony at Entomology 2014, ESA,s Annual ...
(Date:8/28/2014)... news release is available in German . ... water fish, owes its name to a striking pattern of ... types, black cells, reflective silvery cells, and yellow cells emerge ... and arrange as a multilayered mosaic to compose the characteristic ... three cell types have to interact to form proper stripes, ...
(Date:8/28/2014)... A Mayo Clinic researcher and his collaborators have ... and enhance the process of re-engineering cells for biomedical ... network biology methods to aid stem cell engineering. Details ... are described in two back-to-back papers in the journal ... broad range of uses for all types of cell-based ...
Breaking Biology News(10 mins):Fred Baxendale selected as Honorary Member of the Entomological Society of America 2How the zebrafish gets its stripes 2How the zebrafish gets its stripes 3New tool aids stem cell engineering for medical research 2
... gradual extinction of cold-loving species, and the European land ... from the University of Kassel in Germany and the ... that human-induced temperature increases over a 40-year period in ... the near extinction of the local land leech Xerobdella ...
... have long known that the human body runs like clockwork, ... of light and darkness. Now a team of researchers is ... activity, which could allow them to predict optimal timing for ... solar day and relieve psychosocial stress. In short, ...
... showing that dog-walking in bushland significantly reduces bird ... against the practice in sensitive bushland and conservation ... the ecological impacts of dog-walking have remained subjective ... But the first clear evidence that birds ...
Cached Biology News:Role reversal as humans suck life out of leeches 2Researchers developing device to predict proper light exposure for human health 2Researchers developing device to predict proper light exposure for human health 3A dog in the hand scares birds in the bush 2
(Date:8/28/2014)... Ind. A new research platform uses ... of tiny structures undergoing stress and heating, ... improve designs for microelectronics and batteries., This ... information about how heating and the surface ... properties. Researchers have discussed the merits of ...
(Date:8/28/2014)... , Aug. 28, 2014 /PRNewswire/ ... new market research report is ... Whole Exome Sequencing Market ... Preparation, Target Enrichment), by Services ... Synthesis), by Application (Cancer, Monogenic ...
(Date:8/28/2014)... 28, 2014  Next month, executives from clinical trial marketing ... events beginning with Patient-Centered Clinical Trials 2014 , to ... Boston , September 4-5. Patient recruitment experts Bonnie ... Fleishman will share insights on the benefits of employing ... tactics – from media to mobile apps – can be ...
(Date:8/28/2014)... (PRWEB) August 28, 2014 Sterlitech is ... polymeric membranes to its stable of products . ... molecular weight cut-offs and include membrane filters with surface ... increase in customer demand for our membrane process testing ... variety of flat sheet membranes,” explains Sterlitech President Mark ...
Breaking Biology Technology:New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 2Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 4Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 5Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 6Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 7Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 8Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 9Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 10Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 11Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 12Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 13BBK Worldwide Leads Sessions at Key September Events 2Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2
... Inc.  (OTCBB: MDXG), an integrated developer, manufacturer and marketer ... human amniotic membrane, announced today its results for the ... The Company recorded record revenue for the ... quarter of 2011 revenue of $1,043,000 and a six-fold ...
... Pa., July 22, 2011 Janssen Biotech, Inc. ... Administration (FDA) issued a Complete Response letter for ... (golimumab).  The application, filed in September 2010, seeks ... inhibiting the progression of structural damage, inducing major ...
... Inc. (Nasdaq: NGSX ), a biopharmaceutical company ... that on July 21, 2011 it entered into a ... to a group of institutional accredited investors and, subject ... $20 million in gross proceeds from the sale of ...
Cached Biology Technology:MiMedx Group Announces Second Quarter 2011 Results 2MiMedx Group Announces Second Quarter 2011 Results 3MiMedx Group Announces Second Quarter 2011 Results 4MiMedx Group Announces Second Quarter 2011 Results 5MiMedx Group Announces Second Quarter 2011 Results 6MiMedx Group Announces Second Quarter 2011 Results 7MiMedx Group Announces Second Quarter 2011 Results 8MiMedx Group Announces Second Quarter 2011 Results 9MiMedx Group Announces Second Quarter 2011 Results 10FDA Issues Complete Response Letter to Janssen Biotech, Inc. for SIMPONI® Supplemental Biologic License Application 2FDA Issues Complete Response Letter to Janssen Biotech, Inc. for SIMPONI® Supplemental Biologic License Application 3FDA Issues Complete Response Letter to Janssen Biotech, Inc. for SIMPONI® Supplemental Biologic License Application 4FDA Issues Complete Response Letter to Janssen Biotech, Inc. for SIMPONI® Supplemental Biologic License Application 5FDA Issues Complete Response Letter to Janssen Biotech, Inc. for SIMPONI® Supplemental Biologic License Application 6NeurogesX, Inc. Announces $20 Million Private Placement 2NeurogesX, Inc. Announces $20 Million Private Placement 3NeurogesX, Inc. Announces $20 Million Private Placement 4
... The CEQ 8800 Genetic Analysis System, which ... This automated system adds a higher level ... The CEQ 8800, part of Beckman Coulters new ... coding link with the Biomek series liquid handers, ...
... cDNA Library Panels were designed for full-length ... of PCRs to identify the desired cDNA ... in a 96-well "Master Plate," where each ... Having identified the positive well(s) by gel ...
... Rapid-Screen cDNA Library Panels were designed for ... sets of PCRs to identify the desired ... performed in a 96-well "Master Plate," where ... clones. Having identified the positive well(s) by ...
... Library Panels were designed for full-length gene ... PCRs to identify the desired cDNA clone. ... a 96-well "Master Plate," where each well ... identified the positive well(s) by gel electrophoresis ...
Biology Products: