Navigation Links
Molecular movements of neural transporters unveiled
Date:4/25/2011

method does not simply generate information about the average movements of a collection of proteins. "Applying single-molecule imaging to the transporter gave us a unique view of the dynamics enabling the function of the transporters, because it allowed us to look at individual molecules and watch their movements in real time rather than time and ensemble averages," Dr. Blanchard says.

Last year, the researchers used smFRET to collect the first such single-molecule data for a membrane protein, and these results were also published in Nature. In their most recent experiments, they used the technique to monitor changes in LeuT conformation and dynamics by labeling moving parts of the protein with fluorescent dyes that emit distinct amounts of light when the distance separating them changes. As the transporter protein moves during function, time-dependent changes in distance between the fluorophores could be directly imaged to extract the first quantitative insights into the motions underpinning the transport mechanism.

Using powerful computational simulations, the researchers had predicted such movements through previous studies aimed at understanding how the transported molecule changes the conformation of LeuT. The new experiments demonstrated that alanine binding to LeuT increased the rate of the transporter's flickering between two conformations: facing outward, as if ready to accept substrates from outside the cell ("inward-closed"), and facing inward, as if releasing its contents into the cell ("inward-open"). How the presence of sodium affects the transporter's response to the binding of the transported substrate, alanine, was also revealed from these experiments: Sodium was essential for the alanine-enhanced dynamics. Surprisingly, alanine did not alter the total amount of time spent in either the open or closed state.

By contrast, the binding of sodium ions alone, without alanine, was found to decrease the transition rate between o
'/>"/>

Contact: Andrew Klein
ank2017@med.cornell.edu
212-821-0560
New York- Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. SNM releases new fact sheet on breast cancer and molecular imaging
2. Genetic Engineering & Biotechnology News reports on growing role of molecular diagnostics
3. A new journal where molecular biology meets clinical research
4. Scientists trace molecular origin of proportional development
5. New molecular insight into vertebrate brain development
6. Plants display molecular amnesia
7. Pitt, NETL researchers report molecular chain reaction thought to be impossible
8. Tracking the molecular pathway to mixed-lineage leukemia
9. Researchers compile molecular manual for 100s of inherited diseases
10. Biologists learn structure, mechanism of powerful molecular motor in virus
11. Phoenix conference highlights TGens and Scottsdale Healthcares contributions to molecular oncology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/11/2014)... 1 can inhibit voltage-gated calcium channel, decrease ... However, some scholars demonstrated that cannabinoid receptor ... and increase neurotransmitter release. Dr. Yi Zhang ... Tongji Medical College, Huazhong University of Science ... and calcium imaging in cultured trigeminal ganglion ...
(Date:7/11/2014)... brains of all vertebrates, information is transmitted through ... chemical signal to be passed from one brain ... most abundant type of synapse, can be either ... learning, memory, perception and cognition, and the balance ... function. For instance, every time we learn ...
(Date:7/10/2014)... June 27, 2014  Pomerantz LLP has filed ... ("Provectus" or the "Company")(NYSE-MKT: PVCT) and certain of ... States District Court, Middle District of ... of a class consisting of all persons or ... between December 17, 2013 and May 22, 2014, ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3
... San Diego Supercomputer Center (SDSC) at the University ... researchers worldwide unravel the enigmas of phylogenetics, the ... on the planet. The CIPRES Science Gateway ... created by SDSC researchers, allows these studies to ...
... that comes the threat of West Nile virus. Tom Hobman, ... in the Faculty of Medicine & Dentistry, is making every ... , West Nile virus infections often result in flu-like ... show no symptoms at all. But a significant percentage of ...
... new fossil find from the chalk beds of the Franconian ... predatory dinosaur reveals clear traces of fluffy plumage. It also ... The fossil of the fledgling saurian, probably newly hatched ... First of all, juveniles are extremely rare in the dinosaur ...
Cached Biology News:SDSC's CIPRES Science Gateway clarifies branches in evolution's 'tree of life' 2SDSC's CIPRES Science Gateway clarifies branches in evolution's 'tree of life' 3Researchers moving towards ending threat of West Nile virus 2Feathered saurians -- downy dinosaur discovered 2
(Date:7/10/2014)... PETERSBURG, Russia , July 10, 2014 ... Russian Federation Dmitry Medvedev , presented ... biopharmaceutical company BIOCAD . The Company is developing ... scope of MabNext project BIOCAD develops a number of ... cancer and autoimmune diseases. The ceremony took place at ...
(Date:7/10/2014)... D.C., April 15, 2014 -- According to the ... to nuclear security comes from terrorists acquiring sufficient ... to construct a crude nuclear explosive device. The ... nuclear trafficking have involved gram-level quantities, which can ... , According to a new study appearing this ...
(Date:7/10/2014)... Hall effect in bilayer graphene and shown that this ... electric field. , The fractional quantum Hall effect, ... are exposed to large magnetic fields, is a striking ... behave as a single system. However, while the basic ... of this collective behavior remain not well understood, in ...
(Date:7/10/2014)... new alliance will see the two universities and NPL ... with business and industry and their complementary academic strengths., ... help to shape the scientific priorities of the UK. ... up to 300 high-calibre PhD students, will provide a ... NPL, the potential of the Laboratory will be grown ...
Breaking Biology Technology:Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Sensitive detection method may help impede illicit nuclear trafficking 2Columbia researchers observe tunable quantum behavior in bilayer graphene 2Columbia researchers observe tunable quantum behavior in bilayer graphene 3Universities of Surrey and Strathclyde selected as strategic partners in the future operation of the National Physical Laboratory 2Universities of Surrey and Strathclyde selected as strategic partners in the future operation of the National Physical Laboratory 3
... CEPH ) will hold a public memorial service to ... CEO on February 1, 2011, at 6:00 p.m. at The Franklin ... Dr. Baldino founded Cephalon in 1987 and led the company ... biotechnology companies in the world. He was a recognized pioneer in ...
... 24, 2011 Myriant Technologies, Inc. (Myriant), ... renewable bio-based chemicals, announced today the closing ... from PTT Chemical Group (PTT Chemical), Thailand,s ... http://photos.prnewswire.com/prnh/20091021/NE96331LOGO) Myriant will utilize the ...
... 2011 Cardium Therapeutics (NYSE Amex: CXM ... on December 2, 2010 has been accepted by the ... ) As previously reported, a communication from ... that the company was considered to be noncompliant with ...
Cached Biology Technology:Cephalon to Hold Public Memorial Service to Celebrate the Life of Company Founder, Frank Baldino, Jr., Ph.D. 2Myriant Technologies, Inc. Announces US$60 Million Strategic Equity Investment From PTT Chemical Group 2Myriant Technologies, Inc. Announces US$60 Million Strategic Equity Investment From PTT Chemical Group 3Cardium's Exchange Listing Compliance Plan Accepted by NYSE Amex 2Cardium's Exchange Listing Compliance Plan Accepted by NYSE Amex 3Cardium's Exchange Listing Compliance Plan Accepted by NYSE Amex 4
... it uses a deep-UV deuterium bulb, which provides ... a shutter (controlled via a TTL signal or ... produces a powerful, stable output from 215-400 nm. ... are available, providing a wavelength range of 190-400 ...
... situ detection of apoptosis and mitochondrial membrane ... an early indication of the initiation of ... as a collapse in the electrochemical gradient ... the change in the membrane potential (YD). ...
... The NOVOstar is an exciting product ... Previously, cell-based assays required the use of ... accessibility for scientists in the assay development ... necessary for cell-based assays as well as ...
... One can operate as a Voltage Clamp, as ... Clamp in electrophysiology research applications. Performance in any ... equipment. • This amazing ... in the capabilities of Digital Signal Processing (DSP) ...
Biology Products: