Navigation Links
Molecular chaperone keeps bacterial proteins from slow-dancing to destruction
Date:12/28/2009

ANN ARBOR, Mich.---Just like teenagers at a prom, proteins are tended by chaperones whose job it is to prevent unwanted interactions among immature clients. And at the molecular level, just as at the high school gym level, it's a job that usually requires a lot of energy.

In new research, scientists at the University of Michigan and Howard Hughes Medical Institute have discovered how a protein chaperone called HdeA, which helps protect bacteria like the notorious Escherichia coli from the ravages of stomach acid, saves energy while keeping proteins from forming destructive clumps.

The research is described in a paper published online this week in the Proceedings of the National Academy of Sciences.

Proteins in disease-causing bacteria like E. coli unfold when they land in stomach acid after being accidentally ingested by humans and other animals. This unfolding stops the proteins from working and could spell doom for the bacteria if the chaperone HdeA didn't step in. HdeA works by binding very tightly to the unfolded proteins while the bacteria are in the stomach. By attaching to the bacterial proteins, the chaperone stops them from tangling like slow-dancing teens, which could kill the bacteria.

The researchers discovered how HdeA is then able to let go of the unfolded proteins as the bacteria pass into the small intestine so that the proteins refold instead of clumping together.

"HdeA uses a unique timed-release mechanism," said postdoctoral fellow Tim Tapley, who spearheaded the work. "If the proteins were released all at once they would likely clump together, killing the bacteria. What we found instead is that the chaperone HdeA lets go of them gradually, making it more likely that they fold back up into their proper form than clump together."

While most molecular chaperones consume large amounts of cellular energy in order to function, HdeA instead taps energy freely available in its living environment.

"In this way, HdeA is a bit like a wind powered machine, except that instead of harnessing wind, HdeA uses the energy from pH changes in the surrounding environment as the bacteria move from the acid stomach to the slightly alkaline small intestine," said James Bardwell, in whose lab the work was done. Bardwell is a professor of molecular, cellular and developmental biology and of biological chemistry, as well as a Howard Hughes Medical Institute Investigator.


'/>"/>

Contact: Nancy Ross-Flanigan
rossflan@umich.edu
734-647-1853
University of Michigan
Source:Eurekalert

Related biology news :

1. New molecular regulators of hyperthyroidism and goiter
2. Carnegie Mellon scientists investigate initial molecular mechanism that triggers neuronal firing
3. UC health news: molecular pathway may predict chemotherapy effectiveness
4. New molecular clock from LLNL and CDC indicates smallpox evolved earlier than believed
5. Story ideas from Molecular & Cellular Proteomics
6. Lets talk -- new paradigms in the research of the biomolecular composition of water
7. Scientists unveil structure of molecular target of many drugs
8. Potential new therapeutic molecular target to fight cancer
9. NIH selects LIAI for major study on allergy molecular causes and possible treatments
10. Pennsylvania Hospital surgeon receives grant to develop molecular cardiac surgery
11. Leading cause of death in preemies might be controlled by resetting a molecular switch
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/15/2016)... June 15, 2016 Transparency ... titled "Gesture Recognition Market by Application Market - Global Industry Analysis ... 2024". According to the report, the  global gesture recognition ... 2015 and is estimated to grow at a ... by 2024.  Increasing application of gesture ...
(Date:6/7/2016)... June 7, 2016  Syngrafii Inc. and San ... relationship that includes integrating Syngrafii,s patented LongPen™ eSignature ... This collaboration will result in greater convenience for ... union, while maintaining existing document workflow and compliance ... ...
(Date:6/2/2016)...   The Weather Company , an IBM Business (NYSE: ... capability in which consumers will be able to interact with ... via voice or text and receive relevant information about the ... Marketers have long sought an advertising solution that can create ... relevant and valuable; and can scale across millions of interactions ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... ... , ... In anticipation of AxioMed’s exclusive cleanroom manufacturing facility ... Jake Lubinski will be traveling to Switzerland from December 5-10. Mr. Lubinski will ... Zurich to discuss the benefits of a viscoelastic disc. AxioMed received CE mark ...
(Date:12/2/2016)... -- The Multiple Myeloma Research Foundation (MMRF) today announced that ... —the largest and most comprehensive study driving new genomic ... presented at the 58 th American Society of ... San Diego from December 3-6. The new ... as identify pathways and targets for new drug development. ...
(Date:12/2/2016)... 2016 CytRx Corporation (NASDAQ: CYTR ... oncology, today announced the appointment of Earl Warren ... and private healthcare investor, to its Board of Directors. ... clinical and strategic experience at the highest level," said ... "As one of the world,s leading orthopedic surgeons, Dr. ...
(Date:12/2/2016)... Diego, CA (PRWEB) , ... December 01, 2016 , ... ... data from its Phase I/II clinical trials for AC0010 at the World Conference on ... forward to providing an update on the phase I/II clinical trials for AC0010 in ...
Breaking Biology Technology: