Setting the standards
Both sets of reporting requirements, MIAPE and MIMIx, have been shaped by input from the scientific community and developed to minimise the burden on individual researchers. The MIAPE reporting guidelines are being developed as a range of individual modules, which can be combined as necessary to cover an entire experimental workflow from study design to statistical data analysis. As the first finalised module, the MIMIx guidelines implement the general MIAPE principles for describing molecular interactions and also present recommendations on data deposition prior to publication.
Standards in action
Both sets of reporting requirements were put into practice by Bantscheff et al. in the reporting of a large-scale approach to profile the interaction of protein kinases with small inhibitory molecules. The Cellzome researchers used this method to validate the action of three drugs sharing a particular kinase target and also to identify novel drug targets. The binding information shed light on the drug binding specificities and downstream effects on signalling pathways. The MIAPE and MIMIx-compliant mass spectrometry and interaction data were entered into the EBI-hosted PRIDE, IntAct, and ChEBI databases, and accession numbers are included in the research publication to provide direct access to the proteomic information, mass spectra and molecular interaction data. The interconnected EBI data resources serve not only to hold the direct experimental results, but also set them within a wider biological context, for example, by linking the identified kinases to known functions in the UniProt
|Contact: Anna-Lynn Wegener|
European Molecular Biology Laboratory