Navigation Links
Milestone in live microscopy focus of $2 million NIH grant
Date:5/13/2009

A proposal by a team of UC Davis scientists to develop the world's first electron microscope capable of filming live biological processes has been awarded a $2 million grant from the National Institutes of Health.

The team's plan is to extend the capabilities of a powerful new imaging tool called the dynamic transmission electron microscope or DTEM. These instruments can snap 10 to 100 images per millionth of a second, while capturing details as small as 10 nanometers, or about four times the diameter of a DNA molecule.

If they can be adapted to living, moving systems, DTEMs could achieve resolutions 100 times greater than currently attainable for live processes, enabling scientists to observe and record biological processes at the molecular level.

"A microscope with these capabilities will allow us to make milestone advances in our understanding of diseases like cancer, bacterial or viral infections, and basic biological processes," said the research team's leader, Nigel Browning, a professor of chemical engineering and materials science at UC Davis and a staff scientist at Lawrence Livermore National Laboratory. The instrument he and his colleagues propose to design and build would be capable of attaining resolutions as low as one or two nanometers.

Currently, there are only three DTEMs in use worldwide, none of which are designed for observing living systems. Rather, they are utilized to document such processes as inorganic chemical reactions and the dynamics of materials as they change from one state solid, liquid or gas to another. Browning led the group that developed a DTEM in use at Lawrence Livermore.

DTEMs are advanced versions of transmission electron microscopes (TEMs), which have graced research facilities since the late 1930s. However, rather than taking static photos like their predecessors, DTEMs capture processes in real time by using a pulsed laser to produce very short bursts of electrons to illuminate specimens.

In order to create the third-generation TEM, which Browning and his team are calling a "Bio-DTEM," three new elements must be incorporated into the design: a custom-built system to hold an ultra-thin layer of fluid containing the biological sample to be imaged; a short-exposure-time imaging mode, to avoid the blurring problem created when molecules move through their fluid medium; and a new generation of scientific instrumentation to deliver optimum image contrast for biological samples and to correct image distortions generated by lenses.

"It should be noted that each of the components of our proposed new system are commercially available," said Browning. "Our work will be to bring them all together into a single instrument. With this grant from the NIH, I'm confident that we will be able to do so."

Five UC Davis colleagues will collaborate with Browning on the project: Peter Armstrong, Jodi Nunnari and Jon Scholey, professors of molecular and cellular biology; Wolf Heyer, professor of microbiology; and Stephen Kowalczykowski, distinguished professor of microbiology. In July, Browning will add an appointment in the Department of Molecular and Cellular Biology to his academic positions at UC Davis.


'/>"/>

Contact: Liese Greensfelder
lgreensfelder@ucdavis.edu
530-752-6101
University of California - Davis
Source:Eurekalert  

Related biology news :

1. A new milestone in the GMES Space Component Program successfully achieved
2. Secure Services Corp. Achieves Milestone in United States Identity Protection with the Launch of the SSC SHAPE Card Management Solution
3. A gentle touch for better control, a quantum mechanical con, and milestone PRL papers
4. Missouri Botanical Garden mounts milestone 6 millionth herbarium specimen
5. Milestone achieved toward production of malaria treatment using synthetic biology and fermentation
6. ExcellGene and Khner achieved milestone in development of novel 250 Liter mammalian cell culture bioreactors
7. $1.1 million NSF grant to fund research in advanced light microscopy at UCLA
8. Next generation microscopy: No stain, big gain
9. Food and environmental sustainability focus of ASA-CSSA-SSSA Annual Meetings
10. New south Florida nursery to focus on staghorn corals
11. Critically endangered porpoise is focus of new research report
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Milestone in live microscopy focus of $2 million NIH grant
(Date:5/16/2016)... 16, 2016   EyeLock LLC , a market ... opening of an IoT Center of Excellence in ... the development of embedded iris biometric applications. ... convenience and security with unmatched biometric accuracy, making it ... from DNA. EyeLock,s platform uses video technology to deliver ...
(Date:5/9/2016)... UAE, May 9, 2016 Elevay ... comes to expanding freedom for high net worth professionals ... in today,s globally connected world, there is still no ... could ever duplicate sealing your deal with a firm ... passports by taking advantage of citizenship via investment programs ...
(Date:4/28/2016)... FRANCISCO and BANGALORE, India , ... of EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... provider, today announced a global partnership that will ... way to use mobile banking and payment services. ... is a key innovation area for financial services, but it ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... June 23, 2016 Houston Methodist Willowbrook ... Cy-Fair Sports Association to serve as their official ... Houston Methodist Willowbrook will provide sponsorship support, athletic ... with association coaches, volunteers, athletes and families. ... Cy-Fair Sports Association and to bring Houston Methodist ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , ... secured $1 million in debt financing from Silicon Valley ... up automation and to advance its drug development efforts, ... new facility. "SVB has been an incredible ... the services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... 2016 Apellis Pharmaceuticals, Inc. today announced ... of its complement C3 inhibitor, APL-2. The trials ... dose studies designed to assess the safety, tolerability, ... in healthy adult volunteers. Forty subjects ... single dose (ranging from 45 to 1,440mg) or ...
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, the only ... Center and will showcase its product’s latest features from June 26 to June ... scientific poster on Disrupting Clinical Trials in The Cloud during the conference. ...
Breaking Biology Technology: