Navigation Links
Microprinting leads to low-cost artificial cells
Date:12/16/2013

Easily manufactured, low-cost artificial cells manufactured using microprinting may one day serve as drug and gene delivery devices and in biomaterials, biotechnology and biosensing applications, according to a team of Penn State biomedical engineers. These artificial cells will also allow researchers to explore actions that take place at the cell membrane.

"In a natural cell, so much is going on inside that it is extremely complex," said Sheereen Majd, assistant professor of biomedical engineering. "With these artificial cells -- liposomes -- we have just the shell, which gives us the ability to dissect the events that happen at the membrane."

Understanding how drugs and pathogens cross the cell membrane barrier is essential in preventing disease and delivering drugs, and researchers have created artificial cells for quite some time. However, Majd's team is creating large arrays of artificial cells, made of lipids and proteins, of uniform size that can either remain attached to the substrate on which they grow, or become separated and used as freely moving vessels. The researchers report the results of their work today (Dec. 15) in Advanced Materials.

"The trend in the pharmaceutical industry today is that they like to do high throughput screenings," said Majd. "They could use a large number of these artificial cells all of the same size with the same conditions in an array and monitor many cells at once." The researchers' cells are also different because they contain lipids with protein components the way cell membranes exist in nature. The various proteins serve to allow certain materials to enter and leave the cell, acting as regulators.

"These giant proteoliposomes closely mimic cellular membranes," said Majd. "So they are excellent model systems for studying processes that happen at the surface of cells such as the molecular events that occur when pathogens and drugs enter cells." Older methods of artificial cell creation used dried lipids, but in order to create cells with proteins, the system must remain moist because when proteins dry out, they become useless.

Using hydrogel stamping, a process that creates a stamp out of wet hydrogel that deposits dots of the lipid and protein mixture on the surface of the substrate, the researchers can lay out an array of potential artificial cell locations. They then apply an AC electric field to the substrate. Where the lipid and protein mixture exists, tiny bubbles form that eventually combine into one artificial cell. The result is an array of artificial cells neatly placed and spaced on the substrate.

"The physical phenomenon on exactly how the AC field creates the bubbles is not yet understood," said Majd. "But the bottom line is that the AC electric field produces agitation that creates the tiny bubbles that merge to form the cells. This process is called electroformation." The variety of lipids and proteins used can vary depending on the ultimate purpose of the artificial cells. The cells that form are between 20 and 50 microns, within the range of natural cells.

"The beauty of this method is that a lot of labs already use liposomes and electroformation," said Majd. "However, traditionally they do not have proteins attached."

Another problem is the traditional method creates artificial cells in tens of sizes situated all over the place, she added. Other methods require complex devices such as microfluidics to create uniformly sized artificial cells. With the hydrogel stamping method, it is easy to control the size of artificial cells and to generate a large number of these cells efficiently.

The researchers would next like to incorporate more than just lipids and proteins into the artificial cells. One possibility is to bind potential drugs to the proteins and lipids.


'/>"/>

Contact: A'ndrea Elyse Messer
aem1@psu.edu
814-865-9481
Penn State
Source:Eurekalert

Related biology news :

1. Rising ocean acidification leads to anxiety in fish
2. Scripps leads first global snapshot of key coral reef fishes
3. Unlikely collaboration leads to discovery of gender-bending plant
4. Bleeding symptom leads scientists to intracellular traffickers role in virus propagation
5. Penn biologists show that generosity leads to evolutionary success
6. Cross-country collaboration leads to new leukemia model
7. Stress early in life leads to adulthood anxiety and preference for comfort foods
8. Fear factor: Missing brain enzyme leads to abnormal levels of fear in mice, reveals new research
9. Cool it, quick: Rapid cooling leads to stronger alloys
10. Cutting-edge bacteria research leads to more effective treatment of complex infections
11. Quest for edible malarial vaccine leads to other potential medical uses for algae
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
(Date:3/29/2016)... , March 29, 2016 LegacyXChange, ... LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased to announce ... used in a variety of writing instruments, ensuring athletes ... originally created collectibles from athletes on LegacyXChange will be ... of the DNA. Bill Bollander , ...
(Date:3/22/2016)... , March 22, 2016 ... report "Electronic Sensors Market for Consumer Industry by Type ... Others), Application (Communication & IT, Entertainment, Home ... Global Forecast to 2022", published by MarketsandMarkets, ... expected to reach USD 26.76 Billion by ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, ... Pennsylvania Convention Center and will showcase its product’s latest features from June 26 ... presenting a scientific poster on Disrupting Clinical Trials in The Cloud during the ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... of intelligent tools designed, tuned and optimized exclusively for Okuma CNC machining centers ... The result of a collaboration among several companies with expertise in toolholding, cutting ...
(Date:6/23/2016)... India , June 23, 2016 ... media market research report to its pharmaceuticals section ... profiles, product details and much more. ... spread across 151 pages, profiling 15 companies and ... available at http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . ...
(Date:6/22/2016)... Research and Markets has announced the addition of the "Biomarkers: ... The global biomarkers market has grown ... market is expected to grow at a five-year compound annual growth ... billion in 2015 to $96.6 billion in 2020. ... 2020) are discussed. As well, new products approved in 2013 and ...
Breaking Biology Technology: