Navigation Links
MicroRNAs in plants: Regulation of the regulator
Date:11/8/2012

xplains. The study was carried out in collaboration with scientists from the Center for Plant Molecular Biology (ZMBP) and the Proteome Center of the University of Tbingen.

The production of micro RNAs from its precursors has already been extensively studied, especially in animal cells. "Micro RNAs in plants have evolved in parallel and independently. We had to assume that they could be processed in a different way," Pablo Manavella explains.

The scientists used a methodical trick to study the activity of micro RNAs in cells of thale cress plants. First, they developed a reporter system based on the bioluminescent protein luciferase from firefly; its DNA was integrated in the plant cells. Secondly, the scientists inserted in the plant genome a fragment of DNA containing a precursor of an artificial micro RNA that specifically inhibits luciferase. These plants thus initially showed no light emission despite containing the genes encoding luciferase. In a mass experiment, the scientists then triggered unspecific mutations in thousands of plants. With the aid of a special hypersensitive camera the few shining plants were sorted out. "In all these individuals some part of the micro RNA pathway must have been damaged so that luciferase was no longer silenced by the artificial micro RNA," says Pablo Manavella.

To identify the genes responsible for the failure in silencing luciferase, the scientists made use of a new technology developed at the Max Planck Institute, which enables the rapid detection of causal mutations by whole-genome sequence analysis. "Just a few years ago, this project would have been difficult to complete within two years. Nowadays, whole genome sequencing is a rapid and affordable method. By combining the screening test on luciferase activity with whole genome sequencing we could reduce the study period from years to several months," Pablo Manavella explains. Among the obtained mutants the scientists identified the phosphata
'/>"/>

Contact: Pablo Manavella
pablo.manavella@tuebingen.mpg.de
49-707-160-11405
Max-Planck-Gesellschaft
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Triage for plants: NYBG scientists develop and test rapid species conservation assessment technique
2. BUSM study shows role of cellular protein in regulation of binge eating
3. Regulation of telomerase in stem cells and cancer cells
4. Probing the roots of depression by tracking serotonin regulation at a new level
5. International regulation curbs illegal trade of caviar
6. Protein involved in DNA replication, centrosome regulation linked to dwarfism, small brain size
7. Research reveals unique solution to gene regulation
8. A new light shed on genetic regulations role in the predisposition to common diseases
9. Major advances in understanding the regulation and organization of the human genome
10. New findings on gene regulation and bone development
11. Study demonstrates cells can acquire new functions through transcriptional regulatory network
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
MicroRNAs in plants: Regulation of the regulator
(Date:7/27/2014)... of "big data," a single computer cannot always find ... be distributed across a cluster of computers that analyze ... Google mine your web history to present you with ... next favorite book or movie. But big data is ... for monitoring brain activity are generating unprecedented quantities of ...
(Date:7/25/2014)... on the land now called California, and many of ... plants they used for food, fuel, tools, and ritual. ... habitat and natural resources. , Frank Lake, an ecologist ... lead a field trip to the Stone ... America,s 99th Annual Meeting, in Sacramento, Cal. this August. ...
(Date:7/25/2014)... (July 24, 2014)The link between autism and disrupted ... puzzle of the disease, and is largely unknown. ... Autism Research Initiative (SFARI), George Washington University (GW) ... offer truly integrative and in-depth answers to these ... , LaMantia, professor of pharmacology and physiology ...
Breaking Biology News(10 mins):New tools help neuroscientists analyze 'big data' 2New tools help neuroscientists analyze 'big data' 3New tools help neuroscientists analyze 'big data' 4New tools help neuroscientists analyze 'big data' 5Fire ecology manipulation by California native cultures 2Fire ecology manipulation by California native cultures 3GW researcher receives grant to answer the how and why of autism during development 2
... July 9, 2012 Bovine spongiform encephalopathy (BSE, or "mad ... portions of the brain to turn sponge-like. This transmissible ... of protein known as a prion, rather than by a ... signs of illness is about 60 months. Little is known ...
... miles at 150mph, how long will it take to reach ... question makes you apprehensive, then you may have mathematics anxiety. ... journal Behavioral and Brain Functions reports that a ... both genders, performance is likely to be affected as a ...
... MAResearchers from Brigham and Women,s Hospital (BWH) have made a ... The team, led by Thomas S. Kupper, MD, chair of ... that high expression of a cell-signaling molecule, known as interleukin-9, ... findings will be published online in the July 8, 2012 ...
Cached Biology News:Study finds 'mad cow disease' in cattle can spread widely in ANS before detectable in CNS 2Girls' mathematics performance more likely to suffer than boys' as a result of mathematics anxiety 2Researchers discover molecule in immune system that could help treat dangerous skin cancer 2
(Date:7/24/2014)... July 24, 2014 -- Keryx Biopharmaceuticals, Inc. (Nasdaq:KERX) (the ... randomized, active control Phase 3 study of Zerenex (ferric ... for the treatment of hyperphosphatemia in patients with end-stage ... binding and iRon delivery with FErric CiTrate in EsrD) ... the American Society of Nephrology ( JASN ). ...
(Date:7/24/2014)... 2014  Asterias Biotherapeutics, Inc. (OTCBB: ASTYV), a ... regenerative medicine, announced today that it will host ... on Tuesday, July 29, 2014, at 4:30 p.m. ... an overview of Asterias, business strategy and product ... http://www.ustream.tv/channel/asterias-biotherapeutics at least 15 minutes before the ...
(Date:7/24/2014)... computers have yet to materialise. Yet, scientists are ... such computers faster. One such approach relies on ... by applying an electric field. A new study ... coupled quantum dots (TQDs) with electrical impulses can ... example, should TQDs be used as quantum information ...
(Date:7/24/2014)... , July 24, 2014 Research and ... "Global Genomics and Proteomics Analytical Instruments Market 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 Genomics is the study of ... study of the structure and functions of proteomes or ... tools and technology. Genomics involves the mapping of genes ...
Breaking Biology Technology:Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4Asterias Biotherapeutics Announces Live Investor Webcast 2Asterias Biotherapeutics Announces Live Investor Webcast 3Asterias Biotherapeutics Announces Live Investor Webcast 4Global Genomics and Proteomics Analytical Instruments Market 2014-2018: Key Vendors are Agilent, Bio-Rad , F. Hoffmann, La Roche, Illumina, PerkinElmer and Thermo Fisher Scientific 2
... is writing a series of articles on raising capital from investors. ... Most of the time when raising equity capital, the offering terms ... sheet . When dealing with angel investors , it ... , ,Sometimes prospective angel investors will want to negotiate the initial ...
... Oren Harari is coming to Milwaukee next week for ... , ,Not just to manufacturers, who he will be addressing at ... organizations in a number of sectors, including technology. , ,Based on ... in a Copycat Economy , he intends to deploy some provocative ...
... - Mithridion, Inc ., a biopharmaceutical start up that ... has closed an additional $1.2 million of Series A funding ... round of funding brings the total equity capital raised in ... initial funding target. Mithridion will use the funds to develop ...
Cached Biology Technology:Capital-raising term sheets for angels and venture capitalists 2Capital-raising term sheets for angels and venture capitalists 3Capital-raising term sheets for angels and venture capitalists 4Capital-raising term sheets for angels and venture capitalists 5Visions: Innovating like Madonna and Willie Nelson (and Steve Jobs) 2Visions: Innovating like Madonna and Willie Nelson (and Steve Jobs) 3Alzheimer's drug developer lands $1.2M in venture funding 2Alzheimer's drug developer lands $1.2M in venture funding 3
BD BaculoGold Max-XP Insect Cell Medium 1 Liter...
Performance, mycoplasma, virus, and endotoxin tested...
... 10X BlueJuice Gel ... for easy loading and ... in agarose or native ... buffer features higher density ...
... Cloning is the most ... cloning PCR products. TOPO ... Blunt TOPO vectors are ... with topoisomerase I. This ...
Biology Products: