Navigation Links
MicroRNAs in plants: Regulation of the regulator
Date:11/8/2012

xplains. The study was carried out in collaboration with scientists from the Center for Plant Molecular Biology (ZMBP) and the Proteome Center of the University of Tbingen.

The production of micro RNAs from its precursors has already been extensively studied, especially in animal cells. "Micro RNAs in plants have evolved in parallel and independently. We had to assume that they could be processed in a different way," Pablo Manavella explains.

The scientists used a methodical trick to study the activity of micro RNAs in cells of thale cress plants. First, they developed a reporter system based on the bioluminescent protein luciferase from firefly; its DNA was integrated in the plant cells. Secondly, the scientists inserted in the plant genome a fragment of DNA containing a precursor of an artificial micro RNA that specifically inhibits luciferase. These plants thus initially showed no light emission despite containing the genes encoding luciferase. In a mass experiment, the scientists then triggered unspecific mutations in thousands of plants. With the aid of a special hypersensitive camera the few shining plants were sorted out. "In all these individuals some part of the micro RNA pathway must have been damaged so that luciferase was no longer silenced by the artificial micro RNA," says Pablo Manavella.

To identify the genes responsible for the failure in silencing luciferase, the scientists made use of a new technology developed at the Max Planck Institute, which enables the rapid detection of causal mutations by whole-genome sequence analysis. "Just a few years ago, this project would have been difficult to complete within two years. Nowadays, whole genome sequencing is a rapid and affordable method. By combining the screening test on luciferase activity with whole genome sequencing we could reduce the study period from years to several months," Pablo Manavella explains. Among the obtained mutants the scientists identified the phosphata
'/>"/>

Contact: Pablo Manavella
pablo.manavella@tuebingen.mpg.de
49-707-160-11405
Max-Planck-Gesellschaft
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Triage for plants: NYBG scientists develop and test rapid species conservation assessment technique
2. BUSM study shows role of cellular protein in regulation of binge eating
3. Regulation of telomerase in stem cells and cancer cells
4. Probing the roots of depression by tracking serotonin regulation at a new level
5. International regulation curbs illegal trade of caviar
6. Protein involved in DNA replication, centrosome regulation linked to dwarfism, small brain size
7. Research reveals unique solution to gene regulation
8. A new light shed on genetic regulations role in the predisposition to common diseases
9. Major advances in understanding the regulation and organization of the human genome
10. New findings on gene regulation and bone development
11. Study demonstrates cells can acquire new functions through transcriptional regulatory network
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
MicroRNAs in plants: Regulation of the regulator
(Date:4/23/2014)... are turning away from using triclosan as an antimicrobial ... concerns. And now scientists are reporting new evidence that ... the ACS journal Chemical Research in Toxicology , ... called octylphenol, promoted the growth of human breast cancer ... mice. , Kyung-Chul Choi and colleagues note that hormonal ...
(Date:4/23/2014)... first cause of dementia and affects some 400,000 people ... been found. One of the reasons for this is ... cause alterations in nerve transmissions and the loss of ... from the Institute of Neuroscience at the Universitat Autnoma ... memory consolidation and were able to develop a gene ...
(Date:4/23/2014)... technique used to read quickly. It involves visual ... words and/ or sentences. Similarly to humans, biological ... "read" genetic information. Genes that need to be ... the encoding message, the easier it will be ... Gulbenkian de Cincia (IGC, Portugal) and Centre for ...
Breaking Biology News(10 mins):In lab tests, the antimicrobial ingredient triclosan spurs growth of breast cancer cells 2Loss of memory in Alzheimer's mice models reversed through gene therapy 2Cell division speed influences gene architecture 2
... Inc., (formerly Automated Vending Technologies) (Ticker: AVTCD.PK) ( ... automated retailing industries by offering an ATM-style kiosk ... 24-hours a day, without the need for a ... system uses advanced biometric fingerprint recognition and a ...
... University Medical Center researchers have found a genetic variant ... after a coronary artery bypass. The scientists ... two different sets of patients, with about 1,000 patients ... "After the second analysis, we were ecstatic to see ...
... September 13, 2011 New research conducted at the ... a healthy liver. The pilot study, conducted by Dr. Keiko ... and Life Sciences, showed that healthy laboratory rats fed a ... pure maple syrup from Canada yielded significantly better results in ...
Cached Biology News:Gene linked with death after coronary bypass surgery 2Researchers uncover a potential new benefit of pure maple syrup on liver health 2
(Date:1/15/2014)... CA (PRWEB) January 15, 2014 ... research solutions, today announced that Lupin Limited, one of ... Freeslate’s CM Protégé PharmD System for high ... Mumbai, India, is focused on a wide range of ...
(Date:1/14/2014)... 2014 Date: Friday, April 11, 2014 , ... Club, 1360 Almshouse Road, Warrington, Pa. , Details: ... dedicated to finding a cure for hepatitis B and improving ... its annual Crystal Ball on Friday, April 11 at Warrington ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider of ... regulatory, business, and media events in the United States ... former associate Virginia Cox , JD, is returning to the ... Virginia Cox re-joins 3D after more than two years of ...
(Date:1/14/2014)... The largest international professional organization of scientists ... derivatives thereof has endorsed an educational program that ... challenges of adulterated herb and botanical products. ... The Society for Medicinal Plant and Natural Product ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8
... Fisher,Scientific Inc. (NYSE: TMO ) today ... a leading third-party logistics provider to the,pharmaceutical ... was previously majority-owned by Arsenal Capital,Partners. With ... Priority,Solutions offers a comprehensive range of services ...
... With a special eye for the,details hidden from casual ... G. Aspinall. Photographs of architectural details from beaches,to buildings ... fore. The,collection includes images from seven different countries., ... the,president of Genzyme Genetics, is also a talented artist ...
... - Biomira Inc. (Nasdaq: BIOM ),(TSX: BRA) ... Chief,Executive Officer, is scheduled to present at the ... EDT on Wednesday, October 10. The presentation ... be accessed at: http://www.corporate-ir.net/ireye/confLobby.zhtml?ticker=BIOM&item_id=1 ,657575 ...
Cached Biology Technology:Thermo Fisher Scientific Acquires Logistics Provider to Strengthen Clinical Trials Management Services 2Thermo Fisher Scientific Acquires Logistics Provider to Strengthen Clinical Trials Management Services 3Thermo Fisher Scientific Acquires Logistics Provider to Strengthen Clinical Trials Management Services 4An Uncommon Eye: Photography Exhibit by Biotechnology Executive at BHCC Gallery 2
LECHNER AND LaVECK MEDIUMfor the clonal growth of normal human bronchial and other epithelial cells. Requires additives. With glutamine.Recommended storage condition:oC to 8oCIntended use(s):research...
...
... OrientExpress™ System Modules ... synthesis and directional cloning ... T7 expression vectors ... configuring OrientExpress™ Cloning Systems, ...
... and antibody-based immunoaffinity matrices, such as ... times without compromising antigen-antibody reactivity, the ... agents has precluded the application of ... The CHEMICON Re-Blot Plus Western Blot ...
Biology Products: