Navigation Links
Methods for studying DNA repair and protein modification are featured in CSH Protocols
Date:1/5/2009

COLD SPRING HARBOR, N.Y. (Mon., Jan. 5, 2009) - This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc1_09.dtl) features two articles detailing experimental methods for the analysis of molecular processes involved in DNA repair and post-translational modification of proteins.

Homologous recombination is an important mechanism for the repair of damaged chromosomes. When this occurs, a Displacement Loop, or "D-loop," is formed as the two strands of the DNA molecule are separated and held apart by a third strand of DNA. Patrick Sung's laboratory at Yale University (http://info.med.yale.edu/mbb/sung/) has detailed a method for generating these structures in their article, "Assay for Human Rad51-Mediated DNA Displacement Loop Formation." This reconstituted system provides researchers a biochemical means to dissect the mechanisms of the homologous recombination machinery. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/1/pdb.prot5120).

Sumoylation involves the attachment of Small Ubiquitin-like Modifier or "SUMO" proteins to other proteins in a cell. Sumoylation modifies these target proteins and can affect a variety of activities, including stability, transport, and transcriptional regulation. James Manley's laboratory at Columbia University (http://www.columbia.edu/cu/biology/faculty/manley/) provides "In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays," a protocol for modifying proteins in this manner, allowing one to assess the impact of sumoylation. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/1/pdb.prot5121).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. New manual presents robust, state-of-the-art proteomics methods for teaching and research
2. Cold Spring Harbor Protocols features organ and cell culture methods
3. Deep biosphere research points to new methods for recovering petroleum
4. New lab manual focuses on essential methods for purifying and characterizing proteins
5. Radioactivity: Discover the lowest amounts with new methods
6. Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
7. HapMap browsing and DDDP methods for genetic analysis featured in CSH Protocols
8. Cold Spring Harbor Protocols features methods for analyzing genomes and plant cells
9. Expanded histology methods book covers processing of animal, plant, and microbial tissues
10. Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution
11. Powerful yet reliable proteomics techniques are the focus of a new methods book
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/19/2017)... , April 19, 2017 The ... vendor landscape is marked by the presence of several ... however held by five major players - 3M Cogent, ... companies accounted for nearly 61% of the global military ... companies in the global military biometrics market boast global ...
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced Design ... will feature emerging and evolving technology through its ... Summits will run alongside the expo portion of the ... panels and demonstrations focused on trending topics within 3D ... design and manufacturing event will take place June 13-15, 2017 ...
(Date:4/11/2017)... DUBLIN , Apr. 11, 2017 Research ... Tracking Market 2017-2021" report to their offering. ... The global eye tracking market to grow at ... The report, Global Eye Tracking Market 2017-2021, has been prepared based ... report covers the market landscape and its growth prospects over the ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... October 10, 2017 , ... USDM Life ... for the life sciences and healthcare industries, announces a presentation by Subbu Viswanathan ... The presentation, “Automating GxP Validation for Agile Cloud Platforms,” will present a revolutionary ...
(Date:10/9/2017)... ... 09, 2017 , ... At its national board meeting in North Carolina, ... University’s Departments of Physics and Astronomy, has been selected for membership in ARCS ... for the 2015 Breakthrough Prize in Fundamental physics for the discovery of the accelerating ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... launched its ProxiMeta™ Hi-C metagenome deconvolution product, featuring the first commercially available ... bioinformatics software to perform Hi-C metagenome deconvolution using their own facilities, supplementing ...
(Date:10/5/2017)... ... ... LabRoots , the leading provider of educational and interactive virtual events ... cancer research with a month-long promotion supporting the advancement of breast cancer research with ... promo code PinkRibbon to get 10 percent off their purchase of every the “Cancer ...
Breaking Biology Technology: