Navigation Links
Methods for studying DNA repair and protein modification are featured in CSH Protocols
Date:1/5/2009

COLD SPRING HARBOR, N.Y. (Mon., Jan. 5, 2009) - This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc1_09.dtl) features two articles detailing experimental methods for the analysis of molecular processes involved in DNA repair and post-translational modification of proteins.

Homologous recombination is an important mechanism for the repair of damaged chromosomes. When this occurs, a Displacement Loop, or "D-loop," is formed as the two strands of the DNA molecule are separated and held apart by a third strand of DNA. Patrick Sung's laboratory at Yale University (http://info.med.yale.edu/mbb/sung/) has detailed a method for generating these structures in their article, "Assay for Human Rad51-Mediated DNA Displacement Loop Formation." This reconstituted system provides researchers a biochemical means to dissect the mechanisms of the homologous recombination machinery. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/1/pdb.prot5120).

Sumoylation involves the attachment of Small Ubiquitin-like Modifier or "SUMO" proteins to other proteins in a cell. Sumoylation modifies these target proteins and can affect a variety of activities, including stability, transport, and transcriptional regulation. James Manley's laboratory at Columbia University (http://www.columbia.edu/cu/biology/faculty/manley/) provides "In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays," a protocol for modifying proteins in this manner, allowing one to assess the impact of sumoylation. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/1/pdb.prot5121).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. New manual presents robust, state-of-the-art proteomics methods for teaching and research
2. Cold Spring Harbor Protocols features organ and cell culture methods
3. Deep biosphere research points to new methods for recovering petroleum
4. New lab manual focuses on essential methods for purifying and characterizing proteins
5. Radioactivity: Discover the lowest amounts with new methods
6. Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
7. HapMap browsing and DDDP methods for genetic analysis featured in CSH Protocols
8. Cold Spring Harbor Protocols features methods for analyzing genomes and plant cells
9. Expanded histology methods book covers processing of animal, plant, and microbial tissues
10. Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution
11. Powerful yet reliable proteomics techniques are the focus of a new methods book
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/23/2017)... PUNE, India , March 23, 2017 The report ... Equipment, Touchless Biometric), Industry, and Geography - Global Forecast to 2022", published by ... growing at a CAGR of 29.63% between 2017 and 2022. ... ... Logo ...
(Date:3/22/2017)... YORK , March 21, 2017 ... Marketing Cloud used by retailers such as 1-800-Flowers ... its platform — Product Recommendations and Replenishment. Using Optimove,s ... give more personalized product and replenishment recommendations to ... but also on predictions of customer intent drawn ...
(Date:3/20/2017)... March 20, 2017 PMD Healthcare announces the ... and Wellness Management System (WMS), a remote, real-time lung ... 2010, PMD Healthcare is a Medical Device, Digital Health, ... dedicated to creating innovative solutions that empower people to ... intent focus, PMD developed the first ever personal spirometer, ...
Breaking Biology News(10 mins):
(Date:4/21/2017)... , ... April 21, 2017 , ... ... dedicated to nourishing a range of emerging bio and technology start-ups, is hosting ... 21, 2017. This double event will start with libations and networking at 3:30 ...
(Date:4/20/2017)... (PRWEB) , ... April 20, 2017 , ... USDM ... for the life sciences and healthcare industries, is pleased to announce Holger Braemer ... established USDM subsidiary “USDM Europe GmbH” based in Germany. , Braemer is an ...
(Date:4/20/2017)... ... April 20, 2017 , ... Parallel6™ , the leader in mClinical™ technologies ... they were named one of the 2017 Top 10 eClinical Trial Management Solution ... pharmaceutical industry. , “We take pride in honoring Parallel6 as one of the top ...
(Date:4/19/2017)... ... , ... The Vibrating Orifice Aerosol Generator (VOAG) was developed ... of known diameters for research applications such as for calibrating droplet measuring instruments, ... droplets. , The VOAG requires forcing liquid out of an orifice about ...
Breaking Biology Technology: