Navigation Links
Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
Date:8/5/2009

COLD SPRING HARBOR, N.Y. (Wed., Aug. 5, 2009) Vectors derived from retroviruses are useful tools for long-term gene transfer because they allow stable integration of transgenes and propagation into daughter cells. Lentiviral vectors are preferred because they can transduce non-proliferating cellular targets. These vectors can be engineered to target specific tissues. In the August issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc8_09.dtl), Franois-Loc Cosset and colleagues from Ecole Normale Suprieure de Lyon (http://hvd.ens-lyon.fr/human_virology_dpt) present a method for targeting hematopoietic stem cells using engineered viral vectors. The article, "Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors," is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5276).

Though viral vectors are highly efficient, their use can raise concerns about recombination, immune responses and other safety issues. DNA transposons offer an effective, alternative method for nonviral gene transfer that avoids the safety concerns associated with viral vectors. Use of the "Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells" from Catherine Krull and colleagues at the University of Michigan (http://www.med.umich.edu/cdb/sub_pages/People/krull.htm) provides a method for stable integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise integration into a TA dinucleotide. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5270).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
2. Contraception: progress brings hope for new methods for men
3. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
4. New book presents methods to poke and prod individual molecules
5. RNA-based methods for developmental studies are featured in Cold Spring Harbor Protocols
6. Powerful yet reliable proteomics techniques are the focus of a new methods book
7. Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution
8. Expanded histology methods book covers processing of animal, plant, and microbial tissues
9. Cold Spring Harbor Protocols features methods for analyzing genomes and plant cells
10. HapMap browsing and DDDP methods for genetic analysis featured in CSH Protocols
11. Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/6/2017)... -- Delta ID Inc., a leader in consumer-grade iris scanning ... CES® 2017. Delta ID has collaborated with Gentex Corporation ... of iris scanning as a secure, reliable and convenient ... car, and as a way to elevate the security ... ID and Gentex will demonstrate (booth #7326 LVCC) a ...
(Date:12/22/2016)... 22, 2016 SuperCom (NASDAQ:   ... for the e-Government, Public Safety, HealthCare, and Finance sectors announced today ... been selected to implement and deploy a community-based supportive services program ... California , further expanding its presence in the state. ... This new program, which ...
(Date:12/16/2016)... Research and Markets has announced the addition ... to 2021" report to their offering. ... The biometric vehicle access system market, ... of 14.06% from 2016 to 2021. The market is estimated to ... 854.8 Million by 2021. The growth of the biometric vehicle access ...
Breaking Biology News(10 mins):
(Date:1/20/2017)... , January 20, 2017 ... is one of leading causes of death worldwide. There ... the number of cancer related deaths increased gradually over ... in incidence rate of various cancers continues to drive ... research report by Global Market Insights, Inc. cancer biological ...
(Date:1/19/2017)... HOUSTON , Jan. 19, 2017 ... announced the formation of its Medical/Clinical Advisory Board. ... industry veterans who enhance the range and depth ... development of its novel prenatal diagnostic tests.  These ... and strategic guidance for the company,s product development ...
(Date:1/19/2017)... ... 19, 2017 , ... November Research Group, LLC, a global ... device manufacturers and regulators, is proud to announce the worldwide release of PRIMO ... to provide product vigilance departments with the flexibility and ease of use of ...
(Date:1/19/2017)... ... January 18, 2017 , ... The American ... to update its Data Sharing Policy. Specifically, the nation’s leading informatics experts, said ... to the existing policy. AMIA recommended that NIH earmark funding for researchers to ...
Breaking Biology Technology: