Navigation Links
Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
Date:8/5/2009

COLD SPRING HARBOR, N.Y. (Wed., Aug. 5, 2009) Vectors derived from retroviruses are useful tools for long-term gene transfer because they allow stable integration of transgenes and propagation into daughter cells. Lentiviral vectors are preferred because they can transduce non-proliferating cellular targets. These vectors can be engineered to target specific tissues. In the August issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc8_09.dtl), Franois-Loc Cosset and colleagues from Ecole Normale Suprieure de Lyon (http://hvd.ens-lyon.fr/human_virology_dpt) present a method for targeting hematopoietic stem cells using engineered viral vectors. The article, "Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors," is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5276).

Though viral vectors are highly efficient, their use can raise concerns about recombination, immune responses and other safety issues. DNA transposons offer an effective, alternative method for nonviral gene transfer that avoids the safety concerns associated with viral vectors. Use of the "Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells" from Catherine Krull and colleagues at the University of Michigan (http://www.med.umich.edu/cdb/sub_pages/People/krull.htm) provides a method for stable integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise integration into a TA dinucleotide. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5270).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
2. Contraception: progress brings hope for new methods for men
3. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
4. New book presents methods to poke and prod individual molecules
5. RNA-based methods for developmental studies are featured in Cold Spring Harbor Protocols
6. Powerful yet reliable proteomics techniques are the focus of a new methods book
7. Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution
8. Expanded histology methods book covers processing of animal, plant, and microbial tissues
9. Cold Spring Harbor Protocols features methods for analyzing genomes and plant cells
10. HapMap browsing and DDDP methods for genetic analysis featured in CSH Protocols
11. Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/20/2016)... DALLAS , June 20, 2016 ... criminal justice technology solutions for public safety, investigation, ... by the prisons involved, it has secured the ... Corrections (DOC) facilities for Managed Access Systems (MAS) ... (4) additional facilities to be installed by October, ...
(Date:6/9/2016)... , June 9, 2016  Perkotek an innovation leader in attendance control systems ... seamlessly log work hours, for employers to make sure the right employees are actually ... http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
(Date:6/3/2016)... 3, 2016 Das ... Nepal hat ein 44 ... geprägter Kennzeichen, einschließlich Personalisierung, Registrierung und IT-Infrastruktur, ... Produktion und Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte ... Januar teilgenommen, aber Decatur wurde als konformste ...
Breaking Biology News(10 mins):
(Date:11/30/2016)... and BEIJING , Nov. 30, 2016 ... provider of genomic services and solutions with cutting edge ... it has completed a USD $75 Million [515 Million ... Ltd.,s CMB International Capital Management ( Shenzhen ... Co., Ltd. ("SDIC Innovation") and Shanghai Sigma Square Investment ...
(Date:11/30/2016)... , November 30, 2016 Part of ... ... Aptuit, LLC today announced that it had ... additional 150,000 novel compounds have increased the Screening Collection to ... the hit discovery capabilities of the company. This expansion, complemented ...
(Date:11/30/2016)... , ... November 30, 2016 , ... SSCI, the established ... discuss the implications of the latest FDA guidance on pharmaceutical cocrystals as drug ... in Cambridge, MA. , The event follows the successful November 15th event ...
(Date:11/30/2016)... Triangle Park, NC (PRWEB) , ... November 30, ... ... development company engaged in the development of a new orally administered treatment for ... testing and neuroimaging results of a Phase 2a clinical trial of T3D-959 in ...
Breaking Biology Technology: