Navigation Links
Method reveals new view of human nerve cells, opening door to potential drug targets
Date:3/11/2011

had identified about a dozen receptors on warm sensitive neurons that regulate these nerve cells' activity. But Bartfai wanted to find additional receptors to better understand how the cells function.

To do so, he turned to long-time collaborator University of Pennsylvania Professor James Eberwine. Eberwine had pioneered a number of techniques to identify genes active in individual cells.

Sequencing Single Neurons

Bartfai and Eberwine took a unique approach to indentifying gene activity.

Scientists know a gene is "on" in a cell if its messenger RNA (which carries information from genes to sites of protein synthesis) is present. To study gene activity in warm sensitive cells, Eberwine and Bartfai isolated single cells and extracted their RNA. They then made cDNA copies of the messenger RNAs and determined the sequence of the nucleotide bases (adenine, guanine, cytosine, and thymine) in each cDNA molecule.

By matching the DNA sequences obtained to published sequences, the scientists were able to identify the corresponding genes, and thus which genes are turned "on" in the nerve cells.

The technique differs from commonly used methods for studying gene activity. Typically researchers "pool" neurons of one type and examine them as a group, rather than studying single cells. In addition, current techniques generally rely on searching for active genes using microarraysa technique that relies on the preferential binding of sequences in the messenger RNAs /cDNAs to matching DNA sequences "spotted" on the microarray. However, these methods only detect RNAs for which "probes are present on the microarray," in other words, those that are expected. Also, because of the lower sensitivity of this technique than sequencing, only the cDNAs cells produce in relatively large amounts are detected.

"Using single cells, rather than pooling, and sequencing, rather than microarrays, uncovers many more receptors active
'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Commercial aquatic plants offer cost-effective method for treating wastewater
2. UC Riverside biochemists devise method for bypassing aluminum toxicity effects in plants
3. New lab manual focuses on essential methods for purifying and characterizing proteins
4. Deep biosphere research points to new methods for recovering petroleum
5. Fingerprinting method tracks mercury emissions from coal
6. Iowa State researcher develops new treatment method for canine eye diseases
7. New method provides panoramic view of protein-RNA interactions in living cells
8. Cold Spring Harbor Protocols features organ and cell culture methods
9. Engineer to present leak-proof method for carbon dioxide storage at international conference
10. Pitt researchers use fluorescence to develop method for detecting mercury in fish
11. New holographic method could be used for lab-on-a-chip technologies
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... German . ... risky situations such as exposure to predators. Researchers from ... a long-term study on different populations of great tits ... ambient temperature. High metabolic rates and low temperatures were ... birds were more likely to approach potential predators. , ...
(Date:9/30/2014)... Coral reefs face a suite of perilous threats ... coastal development and climate change, fragile coral ecosystems ... Despite this trend, some species of corals surrounding ... a natural protector in their tropical environment: coral ... Natural History,s Smithsonian Marine Station scientist Seabird McKeon ...
(Date:9/30/2014)... -- Winners of The Economist,s prestigious Innovation awards 2014 ... place at the JW Marriott Hong Kong on Friday, October 10 ... their experiences and the lessons those hold for others. The awards, ... the past decade, will be presented at a ceremony in ... will be the first time the ceremony has come to ...
Breaking Biology News(10 mins):Risky metabolism 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 3Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 4Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 5Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 6Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 7
... like AND and OR Boolean logic gates, producing an output ... in the May issue of Nature Chemical Biology , ... tiny circuits. Study leader Takanari Inoue, Ph.D., an assistant ... the Institute of Basic Biomedical Sciences, Center for Cell Dynamics ...
... of published studies on the impact of bark beetles ... more complete picture of the effect of this destructive ... a wide variety ofand often contradictoryconclusions about the bark ... researchers from the USDA Forest Service,s Pacific Southwest Research ...
... Calif.) Synthetic platelets have been developed by UC ... Research Institute and Sanford-Burnham Institute in La Jolla, Calif. ... Materials in a paper titled "Platelet Mimetic Particles ... the components of blood that allow it to prevent ...
Cached Biology News:Training cells to perform Boolean functions? It's logical 2Training cells to perform Boolean functions? It's logical 3New report examines effects of trees killed by bark beetles on wildfire 2UC Santa Barbara researchers develop synthetic platelets 2
(Date:9/29/2014)... the start of the military conflicts in Iraq and ... United States with traumatic brain injury caused by exposure ... improvised explosive devices, or IEDs. Symptoms of traumatic brain ... headaches and nausea, to more severe impairments in memory ... of Defense has recognized the critical importance and complexity ...
(Date:9/29/2014)... Sept. 29, 2014 Research and Markets ... Market by Product & Services, End-User , Application - ... The global oligonucleotide synthesis market is ... million in 2014, growing at a CAGR of 9.8% ... is categorized on the basis of products and services, ...
(Date:9/29/2014)... global challenges in food security, emerging diseases and ... study published online in Science Express ... University of Arizona College of Agriculture and Life ... team of nine scientists has reviewed progress in ... medicine and environmental management using approaches that consider ...
(Date:9/29/2014)... Research and Markets  has announced the ... and Gas Chromatograph Market by Type , Technology, Spectroscopy, ... & Forecast to 2013 - 2020" report to ... a process analytical instrument that provides chemical composition data ... way by which companies carry out their production and ...
Breaking Biology Technology:Modeling shockwaves through the brain 2Modeling shockwaves through the brain 3Modeling shockwaves through the brain 4Oligonucleotide Synthesis (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) Market - Global Forecast to 2019 2Oligonucleotide Synthesis (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) Market - Global Forecast to 2019 3Evolutionary biology: It's not just for textbooks anymore 2Evolutionary biology: It's not just for textbooks anymore 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 2Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 4
... the appointment of James Stoppert as President and Chief Executive Officer ... ... EON) September 3, 2008 -- Segetis is the first and ... wide range of industrial chemicals and plastics that are ultimately derived ...
... China, Sept. 3 ,Acesys Pharmatech and Provid Pharmaceuticals ... expertise and project management,capabilities of Provid in the ... structure of Acesys in China., The combined ... of medicinal chemistry for clients in the pharmaceutical ...
... tested new molecular tools for stem cell research to direct ... development programmes. , A collaborative team of scientists from ... (NESCI) have developed two synthetic molecules which can be used ... into other forms of tissue. , Their use ...
Cached Biology Technology: Stoppert Named as President and CEO at Breakthrough Renewable Technology Firm Segetis, Inc 2Acesys and Provid Announce Alliance for Medicinal Chemistry Services 2New stem cell tools to aid drug development 2New stem cell tools to aid drug development 3
Conveniently document gels, films and membranes with a high quality thermal printer. , Compact, footprint printer , Supplies 256 gray scale for high resolution, archive-quality thermal film prints...
... ICE-LAP3 Mch3 ... study of enzyme regulation and kinetics, cleavage of target ... 15,000 units/mg protein. One unit is defined ... nmol of Ac-DEVD-pNA Cat. No. 235400 per hour at ...
Request Info...
Bovine Coronary Artery Endothelial Cells (BCAEC) (>500,000 cells)...
Biology Products: