Navigation Links
Metal ions regulate terpenoid metabolism in insects
Date:2/28/2013

d C5 units. Special enzymes mediate chain elongation. These enzymes have attracted the curiosity of scientists at the Max Planck Institute for Chemical Ecology, Jena, and the Leibniz Institute for Plant Biochemistry in Halle. They studied mechanistic alternatives of how chain elongation is regulated.

Metal ions instead of specialized enzymes

Enzymes involved in chain elongation belong to the group of isoprenyl diphosphate synthases. Such an enzyme was isolated from larvae of the horseradish leaf beetle Phaedon cochleariae. It raised the interest of Antje Burse, project group leader in the Department of Bioorganic Chemistry at the Max Planck Institute for Chemical Ecology.

Experiments with larvae in which the enzyme encoding gene was silenced showed that the protein was involved in the formation of the C10 monoterpene chrysomelidial that larvae produce to defend themselves against predators. The larvae accumulate this monoterpene in special glands and release it as a defensive secretion when they are attacked by their enemies, such as ants.

However, surprising results emerged after comprehensive biochemical characterization of the enzyme. "After we had conducted an in vitro analysis of the protein, including measurements of product formation in the presence of different metal ions as co-factors, we were surprised to discover that only geranyl diphosphate (C10), a precursor for the defensive substance chrysomelidial, was produced after addition of cobalt and manganese ions. On the other hand, adding magnesium ions resulted in the formation of farnesyl diphosphate (C15), a potential precursor for juvenile hormones, which is 5 carbon atoms longer," says the scientist. All three metals were found in larval tissue, leading to the assumption that enzyme catalysis is directed by the different metal co-factors in the larvae, whichever is predominant in amount: Towards toxin or hormone − physiologically a major difference.
'/>"/>

Contact: Antje Burse
aburse@ice.mpg.de
49-364-157-1265
Max Planck Institute for Chemical Ecology
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. A new avenue to better medicines: Metal-peptide complexes
2. Earthworms soak up heavy metal
3. Nano-velcro clasps heavy metal molecules in its grips
4. Increase in metal concentrations in Rocky Mountain watershed tied to warming temperatures
5. Measuring mercury levels: Nano-velcro detects water-borne toxic metals
6. Sweet new approach discovered to help produce metal casting parts, reduce toxicity
7. SETAC announces Chris Lee Award for metals research winner, Kevin Brix
8. Onion soaks up heavy metal
9. Bluemetal Architects appoints David Le Penske as Vice President of Health & Life Sciences
10. Law that regulates shark fishery is too liberal: UBC study
11. Gut microbiota regulates bile acid metabolism
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Metal ions regulate terpenoid metabolism in insects
(Date:9/30/2014)... Salk professor and Howard Hughes Medical Institute investigator, ... named recipients in the 2014 round of grants ... the BRAIN (Brain Research through Advancing Innovative Neurotechnologies) ... announced September 30, provides more than $3 million ... years. , The BRAIN Initiative, launched last year, ...
(Date:9/30/2014)... water may increase the risk of stillbirth and ... by a Boston University School of Public Health ... Environmental Health , compared 1,091 PCE-exposed pregnancies ... Cape Cod, Ma., where water was contaminated in ... the installation of vinyl-lined asbestos cement pipes. PCE ...
(Date:9/30/2014)... live in almost any aquatic environment on Earth, but ... species are pushed to the limit. The amount of ... critical for how different species cope in the future, ... of Gothenburg, published in the scientific journal Proceedings ... continues apace thanks to increasing levels of greenhouse gases ...
Breaking Biology News(10 mins):Salk scientists receive $3 million for BRAIN Initiative grant 2Contaminated water linked to pregnancy complications, BU study finds 2Shape up quickly -- applies to fish, too! 2
... in ecological or environmental conservation remains contentious the ... demand that governments regulate and encourage restoration projects; ... corporations makes such interventions politically difficult. However, ... a handful of developing nations, alongside South Africa, ...
... , RIVERSIDE, Calif. Alan McHughen , a ... University of California, Riverside, has been chosen by the ... Sciences to receive a 2011-2012 Jefferson Science Fellowship ... selected for the fellowships this year. Successful ...
... is redefining the rules by which we live and at ... level, several areas of the globe are in danger of ... and maybe, one day, live in floating houses. Depending ... others in their adjustment to the effects of global warming, ...
Cached Biology News:UC Riverside plant biotechnologist receives prestigious Jefferson Science Fellowship 2UC Riverside plant biotechnologist receives prestigious Jefferson Science Fellowship 3Floating houses 2Floating houses 3
(Date:9/30/2014)... , September 30, 2014 ... intuitive browsing of chemistry in documents ... software solutions and consulting services for life science ... information and generate Markush structures from documents. ... organizations intellectual property, however generating and understanding Markush ...
(Date:9/30/2014)... Sept. 30, 2014 FemCap Inc., www.femcap.com ... its receipt of a patent No: US, 8,795,248 B2 ... also received FDA clearance in the U.S., 510(K) K121857, ... in Europe. This patent is related to its menstrual cup ... the company is releasing FemmyCycle Teen, a third menstrual ...
(Date:9/30/2014)... SoundConnect, an award ... dedicated to supporting business communication solutions, including business ... a carrier services specialist has joined ... connected and productive at all times. This growth ... businesses to cloud communications services. On a ...
(Date:9/30/2014)... Back in the 1970’s, many companies calculated their ... which included space for circulation and file storage. “It ... at Nidea Corporate Real Estate / ITRA Global , ... 2000, however, 250 square feet per employee became the ... to 175 to 225 square feet, and over the next ...
Breaking Biology Technology:ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2The FemmyCycle 2SoundConnect Expands Carrier Services Division 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 3
... , ... , , , Antibodies ... arrays) or as biopharmaceutical therapeutics. In,all cases the successful production ... criteria include: protein identification,quantitation and the monitoring of purity and ...
... , ... , , , ... of the Agilent 2100 bioanalyzer in combination,with the Protein 200 Plus ... denaturing gel-elctrophoresis (SDSPAGE).,The results showed that the Agilent 2100 bioanalyzer provides,fast ...
... , ... , , , , ... an siRNA sequence,for a target gene, which are of,high importance ... crucial.,An efficient method to optimize,transfection and gene silencing,is critical for ...
Cached Biology Technology:Quality control of antibodies using the,2100 bioanalyzer and the Protein 200,Plus assay 2Quality control of antibodies using the,2100 bioanalyzer and the Protein 200,Plus assay 3Comparison of different protein,quantitation methods 2Comparison of different protein,quantitation methods 3Comparison of different protein,quantitation methods 4Comparison of different protein,quantitation methods 5Comparison of different protein,quantitation methods 6Comparison of different protein,quantitation methods 7Comparison of different protein,quantitation methods 8Comparison of different protein,quantitation methods 9Confirming gene silencing,mechanism by pGFP/GFP22 ,siRNA co-transfection 2Confirming gene silencing,mechanism by pGFP/GFP22 ,siRNA co-transfection 3Confirming gene silencing,mechanism by pGFP/GFP22 ,siRNA co-transfection 4