Navigation Links
Metabolic fingerprinting: Using proteomics to identify proteins in gymnosperm pollination drops

Proteins are vital parts of living organisms, performing a variety of essential functions such as DNA replication, catabolizing reactions, and responding to stimuli. The complete set of proteins expressed in an organism at a given time, under defined conditions, is known as the proteome. While the genome of an organism remains relatively stable, the proteome is remarkably dynamic, varying from cell to cell and even within a single cell and changing rapidly in response to developmental and environmental cues.

Proteomics is a powerful technique for examining the structure and function of the proteome. For some organisms, proteomics can uncover the relationship between DNA, RNA, and the production of proteinsenabling the comparison of the genome to the proteome. For those organisms that have not yet been sequenced, proteomics facilitates the discovery and identification of proteins. In a new study published in the April issue of Applications in Plant Sciences (available for free viewing at, graduate student Natalie Prior and her colleagues demonstrate the suitability of proteomics in determining the composition of gymnosperm pollination drops.

"The biggest limitation in what we are doing is that there is no published gymnosperm genome," says Prior. "Most of the work on gymnosperms has been anatomical, histological, or morphological. The biochemical perspective is really lacking."

Mediating signaling between the pollen and the ovule, one role of the pollination drop is to provide a germination medium, which can be species specific. Additionally, in some species, anti-microbial proteins have been identified, suggesting that pollination drops provide protection in addition to acting as a landing spot for pollen grains.

"The proteins we are finding are really starting points for other research," says Prior. "We can identify these proteins, but there is a lot more research that can be done once we know what proteins are there."

Identification of the proteins found in pollination drops provides a metabolic fingerprint and thereby informs understanding of seed plant evolution. Comparing the proteomes of different species allows for identification of proteins, elucidating pollen-ovule interactions in gymnosperms.

"We are using proteomics to examine the biological relevance of the proteins that the pollen grain is exposed to in the drop," comments Prior. "It's fascinating to know if any of those proteins are consistent among groups of gymnosperms and what we can learn from that."


Contact: Beth Parada
American Journal of Botany

Related biology news :

1. Soy-based S-equol supplement reduces metabolic syndrome risk factors
2. Circadian rhythms have profound influence on metabolic output, UCI study reveals
3. Hot on the trail of metabolic diseases and resistance to antibiotics
4. Gallbladder shown as potential stem cell source for regenerative liver and metabolic disease
5. From feast to famine: A metabolic switch that may help diabetes treatment
6. Eating grapes may help protect heart health in men with metabolic syndrome, new study suggests
7. Metabolic MAGIC
8. Oxidative stress and altered gene expression occurs in a metabolic liver disease model
9. Lack of nutrients and metabolic syndrome linked to different subtypes of depression
10. Production of 5-aminovaleric and glutaric acid by metabolically engineered microorganism
11. FASEB SRC announces conference: Glucose transport -- Gateway for Metabolic Systems Biology
Post Your Comments:
(Date:6/27/2016)... June 27, 2016 Research and Markets has ... 2016-2020" report to their offering. ... America to grow at a CAGR of 12.28% during ... based on an in-depth market analysis with inputs from industry experts. ... the coming years. The report also includes a discussion of the ...
(Date:6/22/2016)... American College of Medical Genetics and Genomics was once again ... of the fastest-growing trade shows during the Fastest 50 Awards ... Las Vegas . Winners are ... of the following categories: net square feet of paid exhibit ... 2015 ACMG Annual Meeting was ranked 23 out of 50 ...
(Date:6/22/2016)... 2016   Acuant , the leading ... has partnered with RightCrowd ® to ... Management, Self-Service Kiosks and Continuous Workforce Assurance. ... functional enhancements to existing physical access control ... with an automated ID verification and authentication ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... Lemonade Stand Foundation (ALSF), a leading national childhood cancer ... bioinformatics lab, using ,big data, to advance the pace ... Liz Scott , co-executive director of ALSF and Alex,s ... Washington, D.C. , hosted by Vice President ... pediatric cancer research and awareness. The ...
(Date:6/27/2016)... , June 27, 2016  Global demand for ... percent through 2020 to $7.2 billion.  This market ... beverages, cleaning products, biofuel production, animal feed, and ... diagnostics, and biocatalysts). Food and beverages will remain ... by increasing consumption of products containing enzymes in ...
(Date:6/27/2016)... ON , June 27, 2016 /PRNewswire/ - BIOREM Inc. ... has been advised by its major shareholders, Clean Technology ... United States based venture capital ... shares of Biorem (on a fully diluted, as converted ... the disposition of their entire equity holdings in Biorem ...
(Date:6/27/2016)... SAN DIEGO , June 27, 2016  Sequenom, ... company committed to enabling healthier lives through the development ... Supreme Court of the United States ... Federal courts that the claims of Sequenom,s U.S. Patent ... the patent eligibility criteria established by the Supreme Court,s ...
Breaking Biology Technology: