Navigation Links
Media highlights in the Dec. 15 issue of Biophysical Journal

Bethesda, MD - The December 15th issue of Biophysical Journal, published by the Biophysical Society, is now available online. Topics of interest include cardiac contraction, x-ray diffraction, DNA elasticity, optical trapping; single-molecule, ADP-bound cross-bridges; cooperativity, and sarcomere.

Volume 93, Issue 12, December 15, 2007

Effects of Sustained Length-Dependent Activation on In Situ Cross-Bridge Dynamics in Rat Hearts

James T Pearson, Monash University
Mikiyasu Shirai, Hiroshima International University
Hirotsugu Tsuchimochi, National Cardiovascular Center
Daryl O Schwenke, University of Otago
Takayuki Ishida, Hiroshima International University
Kenji Kangawa, National Cardiovascular Center
Hiroyuki Suga, National Cardiovascular Center
Naoto Yagi, JASRI, SPring-8

Keywords: cardiac contraction; interfilament spacing; myosin cross-bridges; x-ray diffraction

The intrinsic ability of the heart to adjust the strength of its contractions to the amount of blood filling the heart has been the subject of much interest, and even more speculation. This fundamental mechanism that regulates heart work with every single beat is vital to our existence. Breakdown of this cellular mechanism of control leads to lung congestion and heart failure. The force of contraction is essentially determined by the interactions of two large proteins, actin and myosin. Our group now demonstrates that the cellular mechanism involves sensing the spacing between the filaments of myosin and actin as the heart fills with blood. The strength of contraction is then adjusted according to the stretch of the proteins within the muscle fibres. We have been able to do this by rapidly analyzing the scatter of pulses of synchrotron x-ray radiation off the muscle fibres of the intact heart as it contracts spontaneously. Many other proteins are known to be associated with actin and myosin, and important for structural organization. Surpassing other studies of isolated heart muscle, our work shows that other proteins must be responsible for active regulation of the spacing between the muscle proteins and the strength of contractions.


Elasticity of short DNA molecules: theory and experiment for contour lengths of 0.6-7 m.

Yeonee Seol, JILA
Jinyu Li, University of Colorado at Boulder
Philip Nelson, University of Pennsylvania
Thomas T. Perkins, University of Colorado, Boulder
M. D. Betterton, University of Colorado, Boulder

Keywords: DNA elasticity; force-extension behavior; optical trapping; single-molecule; stretching DNA; worm-like chain

DNA, the biomolecule that provides the blueprint for life, has a lesser-known identity as a stretchy polymer. The authors have found a flaw in the most common model for DNA elasticity, a discovery that will improve the accuracy of single-molecule research and perhaps pave the way for DNA to become an official standard for measuring picoscale forces, a notoriously difficult challenge.

The experiments described in this paper reveal that a classic model for measuring the elasticity of double-stranded DNA leads to errors when the molecules are short. For instance, measurements are off by up to 18 percent for molecules 632 nanometers long, and by 10 percent for molecules about twice that length. (By contrast, the DNA in a single human cell, if linked together and stretched out, would be about 2 meters long.)

The old elasticity model assumes that polymers are infinitely long, whereas the most popular length for high precision single-molecule studies is 600 nm to 2 microns, coauthor Tom Perkins says. Accordingly, several university collaborators developed a new theory, the finite worm-like chain (FWLC) model, which improves accuracy by incorporating three previously neglected effects, including length.

The work described in this article is part of a NIST project studying the possible use of DNA as a picoforce standard, because enzymes build DNA with atomic precision. DNA already is used informally to calibrate atomic force microscopes. An official standard could, for the first time, enable picoscale measurements that are traceable to internationally accepted units. DNA elasticity could provide a force standard from 0.1 -10 pico-Newtons (pN), where 1 pN is the approximate weight of an E. coli cell or the force exerted by 1 milliwatt of light reflected off a mirror.

The work was supported by the Alfred P. Sloan Foundation, a Burroughs Wellcome Fund Career Award in the Biomedical Sciences, the Butcher Foundation, a W.M. Keck Grant in the RNA Sciences, NIST, and the National Science Foundation.

Non-Linear Force-Length Relationship in the ADP-Induced Contraction of Skeletal Myofibrils

Yuta Shimamoto, Waseda University
Fumiaki Kono, Waseda University
Madoka Suzuki, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University
Shin'ichi Ishiwata, Waseda University

Keywords: ADP-bound cross-bridges; cooperativity; dextran; lattice spacing; length-dependent activation; sarcomere

Muscle is one of the most important organs in biological system, yet its regulatory mechanism is still not fully revealed because of its complex nature involving binding of both Ca2+ and myosin (a linear force-generating motor) to actin filament (the track protein). In this work the authors approached the mechanism of regulation by myosin molecules from a new angle. The idea was to test the effect of actin-myosin interaction on force regulation independently of Ca2+, utilizing artificial activation by ADP, a product of ATP hydrolysis, in the absence of free Ca2+. The authors carefully examined the properties of activation by simultaneously monitoring both individual sarcomeres using an inverted phase-contrast microscope, and by measuring their generated force. Contrary to the textbook version of the length-tension relationship, in which the generated force decreases with stretching muscle above its optimum length, a larger active force was generated at longer muscle lengths. Furthermore, the authors linked this cooperative force enhancement with the changes in three-dimensional structure of sarcomeres by monitoring the distance between the actin and myosin filaments. The authors find that the developed force is significantly modulated by tiny (less than 1 nm) changes in the interfilament distance. This property must be characteristic of striated muscles, in which the two filaments form a liquid crystal-like array. This discovery suggests a new chapter in our understanding of the regulation of muscle contraction by narrowing the focusing on the myosin molecules. This is a significant advance in the regulation of muscle contraction that was pioneered in 1972 by Professor M. Endo.


Contact: Ellen R. Weiss
Biophysical Society

Related biology news :

1. September Geology and GSA Today media highlights
2. Heart of Herschel to be presented to media
3. Last chance for media to register for ECCO 14 -- the European Cancer Conference
4. October Geology and GSA Today media highlights
5. Media highlights in the November issues of Biophysical Journal
6. November GEOLOGY and GSA TODAY Media Highlights
7. Nanotechnology and the media: The inside story
8. December GEOLOGY and GSA TODAY media highlights
9. Better protection for biomedial devices could result from Rutgers-Camden research
10. Highlights from the September 2007 Journal of the American Dietetic Association
11. AGU journal highlights -- Sept. 6, 2007
Post Your Comments:
(Date:11/2/2015)... 2015  SRI International has been awarded a contract ... services to the National Cancer Institute (NCI) PREVENT Cancer ... expertise, modern testing and support facilities, and analytical instrumentation ... toxicology studies to evaluate potential cancer prevention drugs. ... Cancer Drug Development Program is an NCI-supported pipeline to ...
(Date:10/29/2015)... 2015  Connected health pioneer, Joseph C. Kvedar ... technology-enabled health and wellness, and the business opportunities that ... The Internet of Healthy Things . Long before ... existed, Dr. Kvedar, vice president, Connected Health, Partners HealthCare, ... moving care from the hospital or doctor,s office into ...
(Date:10/27/2015)... SAN JOSE, Calif. , Oct. 27, 2015 /PRNewswire/ ... human interface solutions, today announced that Google has adopted ... family of touch controller solutions to power its newest ... Nexus 6P by Huawei. --> ... ecosystem partners like Google to provide strategic collaboration in ...
Breaking Biology News(10 mins):
(Date:11/27/2015)... Nov. 27, 2015 /PRNewswire/--  Mallinckrodt plc (NYSE: ... that it has closed the sale of its global ... (GBT- NYSE Euronext) in a transaction valued at approximately ... facilities and a total of approximately 1,000 employees spread ... St. Louis area. This entire workforce ...
(Date:11/26/2015)... 2015 ... Research Laboratories, a leading independent and ... has formed a strategic partnership with ... Health for joint work on clinical ... ) , --> ,     ...
(Date:11/25/2015)... Nov. 25, 2015  PharmAthene, Inc. (NYSE MKT: PIP) ... a stockholder rights plan (Rights Plan) in an effort ... carryforwards (NOLs) under Section 382 of the Internal Revenue ... PharmAthene,s use of its NOLs could be substantially ... defined in Section 382 of the Code. In general, ...
(Date:11/25/2015)... HOLLISTON, Mass. , Nov. 25, 2015 ... a biotechnology company developing bioengineered organ implants for life-threatening ... will present at the LD Micro "Main Event" investor ... PT. The presentation will be webcast live and posted ... also be available at the conference for one-on-one meetings ...
Breaking Biology Technology: