Navigation Links
Mechanical regulation of cell substrates effects stem cell development, adhesion
Date:8/1/2010

PHILADELPHIA - Bioengineers at the University of Pennsylvania have created a system to control the flexibility of the substrate surfaces on which cells are grown without changing the surface properties, providing a technique for more controlled lab experiments on cellular mechanobiology, an important step in the scientific effort to understand how cells sense and respond to mechanical forces in their environment.

Researchers created a library of micromolded, hexagonally spaced elastomeric micropost arrays, one to a few microns high, on which they cultivated cells. The micropost system allowed engineers to modulate the rigidity and flexibility of the substrate surface without changing the adhesive or other material surface properties that could affect cell growth. Post height determined the degree to which a post would bend in response to a cell's horizontal traction force. The system enabled researchers to map cell traction forces to individual focal adhesions and spatially quantify sub-cellular distributions of focal-adhesion area, traction force and focal-adhesion stress.

The research, published in the current issue of the journal Nature Methods, demonstrated that the height of the posts determined the flexibility of the surface substrate, which in turn impacted the cell's morphology, leading to differences in focal adhesions, cytoskeletal contractility and stem-cell differentiation. Furthermore, early changes in cytoskeletal contractility measured by the devices predicted lineage fate decisions made days later by the stem cells.

"The library of micropost arrays spanned a more than 1,000-fold range of rigidity from 1.31 nN μm−1 up to 1,556 nN μm−1," said Chris Chen, lead author and the Skirkanich Professor of Innovation in Bioengineering in the School of Engineering and Applied Science at Penn. "Furthermore, the micropost array library will be made available to researchers in other laboratories."
'/>"/>

Contact: Jordan Reese
jreese@upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Cellular mechanical forces may initiate angiogenesis
2. Scientists create artificial human skin with biomechanical properties using tissue engineering
3. Small mechanical forces have big impact on embryonic stem cells
4. Company Granted Patent on Apparatus, Systems, and Methods for Gathering and Processing Wireless Biometric and Biomechanical Data
5. Clemson researchers advance nano-scale electromechanical sensors
6. Flemish researchers provide the first experimental evidence of dynamic allostery in protein regulation
7. Remote control for cholesterol regulation discovered in brain
8. New computational method to uncover gene regulation
9. New data on the regulation of a protein that is altered in all cancers
10. Rewiring of gene regulation across 300 million years of evolution
11. Gene regulation: Can we stomach it?
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Mechanical regulation of cell substrates effects stem cell development, adhesion
(Date:7/31/2015)... de 2015 BGI llevará a cabo la 10ª ... al 25 de octubre de 2015, en ... su décimo aniversario este año. Desde su inauguración en ... reuniones anuales más influyentes del mundo en los campos ... científicas más dinámicas, entusiastas y amenas. ICG-10 ...
(Date:7/31/2015)... 2015 La 10 th International Conference on ... BGI del 22 al 25 de octubre de 2015 en ... la conferencia celebra su décimo aniversario. Desde su inauguración en ... las reuniones anuales más influyentes del mundo en el campo ... entusiastas y mejores a nivel científico. ICG-10 ...
(Date:7/31/2015)... -- The 10 th International Conference on Genomics (ICG-10, ... 2015, in Shenzhen , China ... th anniversary this year. Since its inauguration in 2006, ... meetings in the ,omics, fields, and is one of the ... ICG-10 focuses on recent breakthroughs and advances in ,omics, research ...
Breaking Biology News(10 mins):La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3The 10th International Conference on Genomics (ICG-10) to Open in October 2
... A. Wells, professor and chairman of the department pharmaceutical ... director of UCSF,s small molecule discovery center, has been ... Biochemistry and Molecular Biology-Merck Award for his pioneering studies ... who also serves on the ASBMB Council, will present ...
... technological innovation with business collaboration to improve conditions for ... new Hunter and Stephanie Hunt Institute for Engineering and ... Gifts totaling $5 million from Hunter and ... B. Lyle and others will establish the institute and ...
... Scientists have designed a synthetic protein that is ... of a native protein, nitric-oxide reductase. , The ... studying nitric-oxide reductase, and for creating biocatalysts for ... Illinois chemistry professor Yi Lu, who directed the ...
Cached Biology News:University of California, San Francisco, researcher receives ASBMB-Merck Award 2New engineering institute to modify, develop technology for the global poor 2New engineering institute to modify, develop technology for the global poor 3Synthetic protein mimics structure, function of metalloprotein in nature 2
(Date:8/28/2015)... NY (PRWEB) , ... August 28, 2015 , ... Since the initial launch of FireflySci ... their quirky humor and excellent customer service. Images such as the Fonz in a ... to add some flavor to the humdrum spectroscopy field. , FireflySci is proud ...
(Date:8/27/2015)... ... ... Capital Edge Consulting , a nationwide consulting firm that specializes in helping ... one of the top 5000 companies listed on the 2015 Inc. 5000 , ... is truly an honor to be recognized as one of America’s fastest growing companies. ...
(Date:8/27/2015)... /PRNewswire/ - Portage Biotech Inc. ("Portage") (OTC Market: ... Holding Company Limited (Biohaven), announced today that dosing ... BHV-0223, a glutamate modulating agent. Biohaven filed an ... obtained clearance from the U.S. Food and Drug ... holds 54% equity interest in Biohaven, a private ...
(Date:8/27/2015)... MENLO PARK, Calif. , Aug. 27, 2015 /PRNewswire/ ... company in the emerging field of regenerative medicine, today ... two investor conferences in the month of September.  ... Officer, will present at Rodman & Renshaw,s 17th Annual ... 10:25am ET/7:25am PT at the St. Regis Hotel in ...
Breaking Biology Technology:Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4Asterias Biotherapeutics to Present at Upcoming Investor Conferences 2
... precisely how fluid boils in tiny "microchannels" has led ... systems to cool high-power electronics in electric and hybrid ... liquid to boil in cooling systems dramatically increases how ... a liquid to below its boiling point, said Suresh ...
... , SAN DIEGO, Sept. 22 SoluLinK, ... the TurboLink Conjugation Catalyst, making conjugation at least 20 times ... peptides and proteins. , , Up to ... conjugation speed for poor yields, poor quality and more labor ...
... , NEW YORK, Sept. 22 ... is available in its catalogue. , , ... and Wireless Systems, Data Processing, EMR Data Transfer) ... , , This report, High-Tech Patient ...
Cached Biology Technology:New findings could help hybrid, electric cars keep their cool 2New findings could help hybrid, electric cars keep their cool 3New findings could help hybrid, electric cars keep their cool 4SoluLinK Introduces Product that Links All Biomolecules Greater Than 20 Times Faster with 100% Yields 2Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 2Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 3Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 4Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 5Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 6Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 7Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 8Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 9Reportlinker Adds High-Tech Patient Monitoring Systems Markets (Remote and Wireless Systems, Data Processing, EMR Data Transfer) Report 10
solute carrier family 25, member 13 (citrin)...
poliovirus receptor-related 2 (herpesvirus entry mediator B), mRNA...
CREB-2 (H-290)...
Anti-Dynamin Monoclonal Antibody Description: 100 g Research Focus: neuroscience Storage: -20C Shipping Temperature: 4C...
Biology Products: