Navigation Links
May's Cold Spring Harbor Protocols features plant gene expression methods, Xenopus imaging
Date:5/3/2010

COLD SPRING HARBOR, N.Y. (Mon., May 3, 2010) -- The generation of transgenic plants can be a lengthy and difficult process. Transient expression assays have been developed as faster and more convenient alternatives for investigating gene function. These assays often take advantage of the ability of Agrobacterium to transfer foreign DNA into plant cells with intact cell walls. Agrobacterium-mediated transformation is, however, inefficient and shows great variability. In the May issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc5_10.dtl), Andreas Nebenfhr and colleagues from the University of Tennessee (http://www.bio.utk.edu/cellbiol/) present "FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species," a quick, efficient and economical assay for gene function in intact plants. The technique involves cocultivation of young plant seedlings and Agrobacterium in the presence of Silwet-77. The Silwet-77 facilitates transformation, thus replacing a wounding or device-dependent vacuum step. As one of May's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/5/pdb.prot5428).


The large size and external development of the frog Xenopus laevis make it an ideal system for in vivo imaging of dynamic cellular activity. Xenopus embryos are amenable to simple genetic manipulation techniques including knockdowns and misexpression, as well as transgenesis. The ease of collecting large numbers of embryos and the larger size of individual cells within an embryo as compared with other vertebrate model systems provides an excellent platform for the observation of cellular behavior and subcellular processes. In the May issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc5_10.dtl), John Wallingford and colleagues from the University of Texas (http://www.bio.utexas.edu/faculty/wallingford/) provide a suite of articles detailing live imaging of Xenopus laevis at low magnification, confocal imaging of fixed tissues, and in one of May's featured articles, "High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology". This protocol describes methods for labeling and high-magnification time-lapse imaging by confocal microscopy and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/5/pdb.prot5427).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Study: Darwin was right to worry that marriage to his cousin affected his offspring
2. Tissue engineering, imaging neuronal circuits featured in Cold Spring Harbor Protocols
3. Top microbiology experts meet in Edinburgh at SGM Spring Conference 2010
4. Stress during pregnancy may increase offsprings risk of asthma
5. Springer editorial board member receives Humboldt Research Award
6. Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
7. Beewolves protect their offspring with antibiotics
8. Springer to partner with the Gesellschaft für Biologische Systematik
9. Will earlier springs throw nature out of step?
10. High-throughput analysis of gene regulation, DNA synthesis in Cold Spring Harbor Protocols
11. Study of shark virgin birth shows offspring can survive long term
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/16/2016)... 16, 2016 The global ... to reach USD 1.83 billion by 2024, according ... Inc. Technological proliferation and increasing demand in commercial ... to drive the market growth.      ... The development of advanced multimodal techniques for biometric ...
(Date:6/7/2016)... , June 7, 2016  Syngrafii Inc. ... a business relationship that includes integrating Syngrafii,s patented ... branch project. This collaboration will result in greater ... the credit union, while maintaining existing document workflow ... http://photos.prnewswire.com/prnh/20160606/375871LOGO ...
(Date:6/2/2016)... Perimeter Surveillance & Detection Systems, ... Infrastructure, Support & Other Service  The latest ... comprehensive analysis of the global Border Security market ... of $17.98 billion in 2016. Now: In ... in software and hardware technologies for advanced video surveillance. ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... June 27, 2016  Global demand for enzymes ... through 2020 to $7.2 billion.  This market includes ... cleaning products, biofuel production, animal feed, and other ... and biocatalysts). Food and beverages will remain the ... increasing consumption of products containing enzymes in developing ...
(Date:6/27/2016)... CA (PRWEB) , ... June 27, 2016 , ... ... for clinical trials, announced today the Clinical Reach Virtual Patient Encounter CONSULT ... care circle with the physician and clinical trial team. , Using the CONSULT module, ...
(Date:6/27/2016)... 27, 2016   Ginkgo Bioworks , a leading ... was today awarded as one of the World ... world,s most innovative companies. Ginkgo Bioworks is engineering ... real world in the nutrition, health and consumer ... with customers including Fortune 500 companies to design ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Researchers at ... most commonly-identified miRNAs in people with peritoneal or pleural mesothelioma. Their findings are the ... read it now. , Diagnostic biomarkers are signposts in the blood, lung fluid ...
Breaking Biology Technology: