Navigation Links
May's Cold Spring Harbor Protocols features plant gene expression methods, Xenopus imaging
Date:5/3/2010

COLD SPRING HARBOR, N.Y. (Mon., May 3, 2010) -- The generation of transgenic plants can be a lengthy and difficult process. Transient expression assays have been developed as faster and more convenient alternatives for investigating gene function. These assays often take advantage of the ability of Agrobacterium to transfer foreign DNA into plant cells with intact cell walls. Agrobacterium-mediated transformation is, however, inefficient and shows great variability. In the May issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc5_10.dtl), Andreas Nebenfhr and colleagues from the University of Tennessee (http://www.bio.utk.edu/cellbiol/) present "FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species," a quick, efficient and economical assay for gene function in intact plants. The technique involves cocultivation of young plant seedlings and Agrobacterium in the presence of Silwet-77. The Silwet-77 facilitates transformation, thus replacing a wounding or device-dependent vacuum step. As one of May's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/5/pdb.prot5428).


The large size and external development of the frog Xenopus laevis make it an ideal system for in vivo imaging of dynamic cellular activity. Xenopus embryos are amenable to simple genetic manipulation techniques including knockdowns and misexpression, as well as transgenesis. The ease of collecting large numbers of embryos and the larger size of individual cells within an embryo as compared with other vertebrate model systems provides an excellent platform for the observation
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. Study: Darwin was right to worry that marriage to his cousin affected his offspring
2. Tissue engineering, imaging neuronal circuits featured in Cold Spring Harbor Protocols
3. Top microbiology experts meet in Edinburgh at SGM Spring Conference 2010
4. Stress during pregnancy may increase offsprings risk of asthma
5. Springer editorial board member receives Humboldt Research Award
6. Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
7. Beewolves protect their offspring with antibiotics
8. Springer to partner with the Gesellschaft für Biologische Systematik
9. Will earlier springs throw nature out of step?
10. High-throughput analysis of gene regulation, DNA synthesis in Cold Spring Harbor Protocols
11. Study of shark virgin birth shows offspring can survive long term
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/27/2014)... West Orange, NJ. August 27, 2014. Using functional ... shown differential brain activation patterns between people with ... the first MS study in which brain activation ... cognitive task. The article, "Neuroimaging and cognition using ... was published online on June 11 by ...
(Date:8/27/2014)... University researchers have identified a gene that could help ... a protein in the cell membrane of plants that ... water conservation machinery accordingly. , "It,s similar to ... biology at Duke. , The findings, which appear ... make it easier to feed the world,s growing population ...
(Date:8/27/2014)... Many African-Americans may not be getting effective doses of ... Hopkins suggests. The initial dosing studies, completed before the ... generally lack a protein that is key to removing ... people with maximum levels of the protein including ... maraviroc in their bodies compared to those who lack ...
Breaking Biology News(10 mins):Kessler Foundation researchers publish first study of brain activation in MS using fNIRS 2Water 'thermostat' could help engineer drought-resistant crops 2Dosage of HIV drug may be ineffective for half of African-Americans 2
... protein (APP), whose cleavage product, amyloid-b (Ab), builds up ... patients, jumps from one specialized membrane microdomain to another ... there is no definitive evidence that Ab plaques are ... circumstantial evidence to support this. And working on this ...
... one step closer to finding a vaccine that better ... at Saint Louis University appears likely to offer significantly ... one in current use. "Not only was it ... better immune response, which suggests it will be more ...
... - one that,s always fatal and that has a long ... in animal models is significant. This week, Johns Hopkins ... cells into rat models of the disease clearly shifts key ... - slowing the animals, neuron loss and extending life. ...
Cached Biology News:Research identifies type of vaccine that holds promise in protecting against TB 2Swamping bad cells with good in ALS animal models helps sustain breathing 2Swamping bad cells with good in ALS animal models helps sustain breathing 3
(Date:8/28/2014)... , Aug. 28, 2014   SunTrust Robinson Humphrey ... industry veterans to its equity research team in ... ongoing expansion in equity research demonstrates our commitment ... insights to enhance their investment decision making," said ... STRH.  "We continue to make significant investments in ...
(Date:8/28/2014)... by a Florida State University research team has led ... temperature, emit white light, and convert photon energy directly ... a butterfly. , Biwu Ma, associate professor in the ... College of Engineering, created the molecule in a lab ... that his creation has many other unique capabilities. , ...
(Date:8/28/2014)... August 28, 2014 Earlier this month, ... in partnership with the U.S. Air Force on ... The results indicated that this type of fuel made ... and will be important to America’s Eco friendly future. ... has a mandate to become independent of foreign oil ...
(Date:8/28/2014)... August 28, 2014 Whitehouse Laboratories is ... term partnership with PTI Inspection Systems that ... state of the art leak testing method development and ... instruments currently available. As part of this agreement, Whitehouse ... Voltage Leak Detection Instrument developed and manufactured by ...
Breaking Biology Technology:SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 2SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 3Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 3Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 4Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 5Whitehouse Labs Renews Partnership with PTI Inspection Systems 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 3
... N .Y. -- Using clever but elegant design, University ... can be used to capture and purify nanomaterials. , ... "bottle-brush molecule," the traps consist of tiny, organic tubes ... enables the tubes to selectively encapsulate only positively charged ...
... 5, 2011 Levi & Korsinsky is investigating potential ... Pacific Biosciences of California, Inc. ("PacBio" or the "Company") ... federal securities laws. For more information, click ... stems from allegations that the Company failed to disclose ...
... (Nasdaq: VPHM ) today announced that Robert Doody, ... the 2011 Deutsche Bank BioFEST Conference at 8:35 A.M. ET ... at the Four Seasons Hotel in Boston. Charles ... present at the Oppenheimer 22 nd Annual Healthcare Conference ...
Cached Biology Technology:Chemists become molecular sculptors, synthesizing tiny, molecular traps 2Chemists become molecular sculptors, synthesizing tiny, molecular traps 3The Law Firm of Levi & Korsinsky, LLP Launches an Investigation Into Possible Securities Laws Violations by Pacific Biosciences of California, Inc. 2
... for use in an automated line. The ... manifold provides greater ease, speed, convenience, and ... II can be connected to a range ... a microplate with one-handed operation -- in ...
... Zyagen tissue microarrays are derived from ... to rigorous quality control procedures to ensure ... are manually assembled in one block, sectioned ... mounted on coated slides. Sections are large ...
...
... 2-D standards consist of seven reduced, denatured ... carbonic anhydrase, rabbit muscle GAPDH, bovine muscle ... with pIs ranging from 4.5 to 8.5 ... 76,000. These 2-D standards provide calibrated references ...
Biology Products: