Navigation Links
May's Cold Spring Harbor Protocols features plant gene expression methods, Xenopus imaging
Date:5/3/2010

COLD SPRING HARBOR, N.Y. (Mon., May 3, 2010) -- The generation of transgenic plants can be a lengthy and difficult process. Transient expression assays have been developed as faster and more convenient alternatives for investigating gene function. These assays often take advantage of the ability of Agrobacterium to transfer foreign DNA into plant cells with intact cell walls. Agrobacterium-mediated transformation is, however, inefficient and shows great variability. In the May issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc5_10.dtl), Andreas Nebenfhr and colleagues from the University of Tennessee (http://www.bio.utk.edu/cellbiol/) present "FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species," a quick, efficient and economical assay for gene function in intact plants. The technique involves cocultivation of young plant seedlings and Agrobacterium in the presence of Silwet-77. The Silwet-77 facilitates transformation, thus replacing a wounding or device-dependent vacuum step. As one of May's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/5/pdb.prot5428).


The large size and external development of the frog Xenopus laevis make it an ideal system for in vivo imaging of dynamic cellular activity. Xenopus embryos are amenable to simple genetic manipulation techniques including knockdowns and misexpression, as well as transgenesis. The ease of collecting large numbers of embryos and the larger size of individual cells within an embryo as compared with other vertebrate model systems provides an excellent platform for the observation of cellular behavior and subcellular processes. In the May issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc5_10.dtl), John Wallingford and colleagues from the University of Texas (http://www.bio.utexas.edu/faculty/wallingford/) provide a suite of articles detailing live imaging of Xenopus laevis at low magnification, confocal imaging of fixed tissues, and in one of May's featured articles, "High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology". This protocol describes methods for labeling and high-magnification time-lapse imaging by confocal microscopy and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/5/pdb.prot5427).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Study: Darwin was right to worry that marriage to his cousin affected his offspring
2. Tissue engineering, imaging neuronal circuits featured in Cold Spring Harbor Protocols
3. Top microbiology experts meet in Edinburgh at SGM Spring Conference 2010
4. Stress during pregnancy may increase offsprings risk of asthma
5. Springer editorial board member receives Humboldt Research Award
6. Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
7. Beewolves protect their offspring with antibiotics
8. Springer to partner with the Gesellschaft für Biologische Systematik
9. Will earlier springs throw nature out of step?
10. High-throughput analysis of gene regulation, DNA synthesis in Cold Spring Harbor Protocols
11. Study of shark virgin birth shows offspring can survive long term
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/24/2017)... WASHINGTON , April 24, 2017 ... counsel and partner with  Identity Strategy Partners, LLP ... "With or without President Trump,s March 6, ... Foreign Terrorist Entry , refugee vetting can be instilled ... refugee resettlement. (Right now, all refugee applications are ...
(Date:4/19/2017)... York , April 19, 2017 ... as its vendor landscape is marked by the presence ... market is however held by five major players - ... Together these companies accounted for nearly 61% of the ... the leading companies in the global military biometrics market ...
(Date:4/18/2017)... Inc., a global expert in SoC-based imaging and computing solutions, has ... features the company,s hybrid codec technology. A demonstration utilizing TeraFaces ® ... be showcased during the upcoming Medtec Japan at Tokyo Big Sight ... Las Vegas Convention Center April 24-27. ... Click here for an image of the ...
Breaking Biology News(10 mins):
(Date:4/27/2017)... ... April 27, 2017 , ... ... mass flow controllers based on capillary thermal mass flow technology provide exponentially more ... control applications. Over 80% of all industrial processes—such as those involving chemical ...
(Date:4/27/2017)... ... April 27, 2017 , ... ... joined other scientists, researchers, engineers, and industry professionals in visiting U.S. Congressional offices ... to compete in the world photonics industry. , This year, National Photonics ...
(Date:4/27/2017)... April 27, 2017  Pendant Biosciences, Inc. (formerly Nanoferix, ... modification and drug delivery technologies, today announced that it ... @ Toronto . ... Pendant Biosciences, noted, "We are excited to become part ... community, and are honored to be the first ...
(Date:4/26/2017)... (PRWEB) , ... April 26, 2017 , ... ... Lajollacooks4u, San Diego’s premiere team-building and cooking events company, offers one-of-a-kind gifts, ranging ... , Menus specialize in California cuisine, and guests leave inspired with new cooking ...
Breaking Biology Technology: