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MU scientist develops salmonella test that makes food safer, reduce recalls
Date:11/22/2010

COLUMBIA, Mo. Earlier this year, an outbreak of salmonella caused by infected eggs resulted in thousands of illnesses before a costly recall could be implemented. Now, University of Missouri researchers have created a new test for salmonella in poultry and eggs that will produce faster and more accurate results than most currently available tests. The new test could have prevented the contaminated eggs from being shipped to stores.

"Processors and consumers will benefit from the speed and sensitivity of the new test's results," said Azlin Mustapha, associate professor of food science in the College of Agriculture, Food and Natural Resources. "This will keep companies from shipping contaminated products, and thus, keep salmonella infected products out of consumers' hands."

Salmonella is the most common cause of food poisoning in the United States, according to the Centers for Disease Control. Salmonellosis, the disease caused by salmonella, causes diarrhea, vomiting, fever, abdominal cramps and, in severe cases, death. Mustapha said salmonella testing in poultry is important because it persists in birds' spleens and reproductive tracts. An infected bird passes the infection on to all of its eggs.

The most commonly used testing method for salmonella can take up to five days to produce results. Mustapha's research allows scientists to use a process, known as polymerase chain reaction (PCR), which can cut testing time to as little as five to 12 hours. PCR-based testing methods for salmonella have been available for use by the food industry for years, but current methods often produce false-positive results because they do not differentiate between live and dead salmonella, thus skewing the accuracy of the test. Only live salmonella cells trigger salmonellosis.

Mustapha modified the PCR test by adding a dye to the test sample. The dye is absorbed by dead salmonella cells; thus, the PCR test can ignore the dead cells. Mustapha's m
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Contact: Christian Basi
BasiC@missouri.edu
573-882-4430
University of Missouri-Columbia
Source:Eurekalert  

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