Navigation Links
MU researchers develop advanced 3-dimensional 'force microscope'

COLUMBIA, Mo. Membrane proteins are the "gatekeepers" that allow information and molecules to pass into and out of a cell. Until recently, the microscopic study of these complex proteins has been restricted due to limitations of "force microscopes" that are available to researchers and the one-dimensional results these microscopes reveal. Now, researchers at the University of Missouri have developed a three-dimensional microscope that will yield unparalleled study of membrane proteins and how they interact on the cellular level. These microscopes could help pharmaceutical companies bring drugs to market faster.

"Force microscopes are very different from the microscopes we used in biology class," said Gavin King, assistant professor of physics and astronomy in the College of Arts & Science at MU, and joint assistant professor of biochemistry. "Instead of using optics, force microscopes incorporate a tiny needle that gets dragged across the surface of the slide or specimen, similar to how a blind person reads Braille or comparable to the needle of an old record player. However, the one-dimensional, traditional method of studying membrane proteins through a force microscopewhile goodonly yields limited results," King said.

Normally, force microscopes measure the compression of the needle against the specimen by bouncing a single laser off the cantilever, or arm, that holds the microscopic needle in place. As the cantilever moves, it deflects light that is sent back to a highly advanced computer. There, the results are interpreted, giving researchers an idea of how the membrane proteins are interacting with the cell.

Usually, to determine membrane protein structure in detail, specimens must be crystallized, or frozen; therefore, the specimen cannot be studied as it would behave in the primarily liquid environment found in the body.

King and his fellow researcher, Krishna Sigdel, a postdoctoral fellow in the Department of Physics, solved the problem by building their own force microscope that is able to study membrane proteins in conditions similar to those found in the body. Using a traditional one-dimensional force microscope as a guide, the team added an additional laser that measures the second and third dimensions of tip movement, giving researchers "real-time" access to the measurement of peaks and valleys in the membrane protein and dynamic changes in those structures.

"By adding a new laser that is focused from below, we essentially gave the force microscope two additional dimensions," King said. "Using this new laser, we collect the back-scattered light from not only the cantilever holding the needle, but also the tip of the needle that gives additional measurements. This added flexibility allows us to collect information faster and allows our microscope to work in near-native conditions in fluid like those found in the cell, yielding more realistic results."

King suggested that an advantage of three-dimensional force microscopy is that it allows for better interpretation of how a protein's dynamic shape also dictates its function. King said that by studying how the shape of proteins change, researchers can determine how drugs bind and interact with cells. Using membrane protein information, pharmaceutical companies can determine which molecules to pursue.


Contact: Jeff Sossamon
University of Missouri-Columbia

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
Related Image:
MU researchers develop advanced 3-dimensional 'force microscope'
(Date:6/2/2016)... YORK , June 2, 2016   The Weather ... is announcing Watson Ads, an industry-first capability in which consumers ... by being able to ask questions via voice or text ... Marketers have long sought ... the consumer, that can be personal, relevant and valuable; and ...
(Date:5/20/2016)... -- VoiceIt is excited to announce its new marketing ... working together, VoiceIt and VoicePass will offer an ... slightly different approaches to voice biometrics, collaboration between ... Both companies ... "This marketing and technology partnership allows VoiceIt ...
(Date:5/3/2016)... 2016  Neurotechnology, a provider of high-precision biometric ... Biometric Identification System (ABIS) , a complete system ... ABIS can process multiple complex biometric transactions with ... fingerprint, face or iris biometrics. It leverages the ... MegaMatcher Accelerator , which have been used ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 23, 2016 , ... Charm Sciences, Inc. is pleased to ... AOAC Research Institute approval 061601. , “This is another AOAC-RI approval of the ... Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods perform comparably ...
(Date:6/23/2016)... ... June 23, 2016 , ... Supplyframe, the Industry Network ... Supplyframe Design Lab . Located in Pasadena, Calif., the Design Lab’s mission is ... projects are designed, built and brought to market. , The Design Lab is ...
(Date:6/23/2016)... BEACH, Calif. , June 23, 2016  Blueprint ... new biological discoveries to the medical community, has closed ... co-founder Matthew Nunez . "We have ... us with the capital we need to meet our ... will essentially provide us the runway to complete validation ...
(Date:6/23/2016)... , ... June 23, 2016 , ... ... quality, regulatory and technical consulting, provides a free webinar on Performing ... July 13, 2016 at 12pm CT at no charge. , Incomplete investigations are ...
Breaking Biology Technology: