Navigation Links
MSU scientists unlock key enzyme using newly created 'cool' method
Date:2/26/2010

EAST LANSING, Mich. A team of Michigan State University scientists using a new cooling method they created have uncovered the inner workings of a key iron-containing enzyme, a discovery that could help researchers develop new medicines or understand how enzymes repair DNA.

Taurine/alpha-ketoglutarate dioxygenase, known as TauD, is a bacterial enzyme that is important in metabolism. Enzymes in this family repair DNA, sense oxygen and help produce antibiotics.

Specifically, the MSU team was interested in how iron and oxygen atoms reacted together in the enzyme. Understanding how TauD works, which serves as a model for many other proteins, has implications in the scientific and medical fields, said Robert Hausinger, MSU professor of microbiology and molecular genetics.

"This is a broad enzyme family with similar mechanisms," he said. "Understanding how TauD works sheds light on how many other enzymes function from bacteria to humans. This can be applicable to a wide variety of essential enzymes of medical and agricultural interest."

For example, Hausinger said, understanding how the enzyme works can help scientists design inhibitors to prevent it from doing its job, which is a key step in preventing diseases. Also, understanding how the iron inserts oxygen atoms into other molecules provides insight into how enzymes metabolize the majority of medical drugs or environmental pollutants in the human body.

As understanding how enzymes work can be very complicated such reactions often are complex, fast and require multiple steps the MSU team developed a new method to follow the TauD reaction. The difficult part for researchers was to slow down the reaction enough that the individual steps can be observed; one way to slow down an enzymatic reaction is to cool it.

The team used a stream of cold nitrogen gas to slow down the reaction at -36 C (-33 F). To prevent freezing and to keep the reaction going, the scientists used ethylene glycol the same antifreeze that goes in vehicles.

Once the reaction started, the team used lasers in an advanced method called Raman spectroscopy to follow the vibrations of iron and oxygen atoms in TauD to determine how the reaction progressed. They found never seen before steps in the TauD reaction, overturning conventional thought.


'/>"/>

Contact: Jason Cody
codyja@msu.edu
517-432-0924
Michigan State University
Source:Eurekalert

Related biology news :

1. Scientists reveal driving force behind evolution
2. Hormone study gives scientists a sense of how animals bond
3. 78 scientists elected to the American Academy of Microbiology
4. U. of Colorado scientists create tiny RNA molecule with big implications for lifes origins
5. George Daley to discuss challenges and opportunities facing stem cell scientists
6. Scientists unlock mystery in important photosynthesis step
7. Scientists identify critical enzyme in healthy heart function
8. Sanford-Burnham scientists identify natural compound that inhibits cancer cell migration
9. Scientists discover molecular pathway for organ tissue regeneration and repair
10. Scientists synthesize unique family of anti-cancer compounds
11. ASU scientists develop universal DNA reader to advance faster, cheaper sequencing efforts
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... June 27, 2016 Research and Markets has ... 2016-2020" report to their offering. ... America to grow at a CAGR of 12.28% during ... based on an in-depth market analysis with inputs from industry experts. ... the coming years. The report also includes a discussion of the ...
(Date:6/22/2016)... , June 22, 2016  The American College of Medical ... Show Executive Magazine as one of the fastest-growing trade ... 25-27 at the Bellagio in Las Vegas ... highest percentage of growth in each of the following categories: ... companies and number of attendees. The 2015 ACMG Annual Meeting ...
(Date:6/22/2016)... 22, 2016   Acuant , the ... solutions, has partnered with RightCrowd ® ... Visitor Management, Self-Service Kiosks and Continuous Workforce ... add functional enhancements to existing physical access ... venues with an automated ID verification and ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 23, 2016   EpiBiome , a precision microbiome ... in debt financing from Silicon Valley Bank (SVB). The ... to advance its drug development efforts, as well as ... "SVB has been an incredible strategic partner to ... traditional bank would provide," said Dr. Aeron Tynes ...
(Date:6/23/2016)... Apellis Pharmaceuticals, Inc. today announced positive ... its complement C3 inhibitor, APL-2. The trials were ... studies designed to assess the safety, tolerability, pharmacokinetics ... healthy adult volunteers. Forty subjects were ... dose (ranging from 45 to 1,440mg) or repeated ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... at the Pennsylvania Convention Center and will showcase its product’s latest features from ... also be presenting a scientific poster on Disrupting Clinical Trials in The Cloud ...
(Date:6/23/2016)... India , June 23, 2016 ... media market research report to its pharmaceuticals section ... profiles, product details and much more. ... spread across 151 pages, profiling 15 companies and ... available at http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . ...
Breaking Biology Technology: