Tampa, Fla. (Nov. 01, 2010) When researchers tested three different labeling agents on three different stem cell populations to determine what effect the labeling agents had on stem cell phenotype, biological behavior and migration abilities, they found changes in stem cell proliferation depending on the type of contrast agent used.
The team of researchers from Belgium and Spain tested USPIO (ultra small superparamagnetic iron oxide) contrast agents Resovist , Endorem and Sinerem on mouse embryonic stem cells (mESC), rat multipotent adult progenitor cells (rMAPC) and mouse mensenchymal stem cells (mMSC). Their study is published in the current issue of Cell Transplantation (19:8), now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/.
The researchers found the labeling efficiency with each of the (U)SPIOs varied significantly when different stem cell populations were compared.
"This means that labeling methods will likely need to be optimized for every cell type," said Dr. Crabbe. "Over time we saw a dilution of (U)SPIOs and a decrease of iron in the cells."
Non-invasive imaging plays an important post-transplantation role in stem cell research, but questions regarding whether the contrast agents used to track transplanted stem cells in vivo via MRI have an impact on the cells had largely gone unanswered until this study.
On the issue of whether (U)SPIO labeling has a biological affects on cells, the researchers discovered "no significant alterations" in cell phenotypes and that the label "does not significantly alter stem cell differentiation."
"Sinerem decreased proliferation of mMSC while both Sinerem and Endorem affected the proliferation rate of rMAPC, although prolonged culture, until seven days, resulted in restoration of the proliferation rate," noted Dr. Crabbe. "We also found that higher concentrations o
|Contact: David Eve|
Cell Transplantation Center of Excellence for Aging and Brain Repair