Navigation Links
MIT sorts cells with beams of light

CAMBRIDGE, Mass. Separating out particular kinds of cells from a sample could become faster, cheaper and easier thanks to a new system developed by MIT researchers that involves levitating the cells with light.

The system, which can sort up to 10,000 cells on a conventional glass microscope slide, could enable a variety of biological research projects that might not have been feasible before, its inventors say. It could also find applications in clinical testing and diagnosis, genetic screening and cloning research, all of which require the selection of cells with particular characteristics for further testing.

Joel Voldman, an associate professor in MITs Department of Electrical Engineering and Computer Science, and Joseph Kovac, a student in the department, developed the new system, which is featured as the cover story in the Dec. 15 issue of the journal Analytical Chemistry.

Present methods allow cells to be sorted based on whether or not they emit fluorescent light when mixed with a marker that responds to a particular protein or other compound. The new system allows more precise sorting, separating out cells based not just on the overall average fluorescent response of the whole cell but on responses that occur in specific parts of the cell, such as the nucleus. The system can also pick up responses that vary in how fast they begin or how long they last.

Weve been interested in looking at things inside the cell that either change over time, or are in specific places, Voldman says. Separating out cells with such characteristics cant be done with traditional cell sorting.

For example, if cells differ in how quickly they respond to a particular compound used in the fluorescent labeling, the new system would make it possible to select out the ones that are faster or slower, and see whats different, says Voldman, who also has appointments in MITs Research Laboratory of Electronics and the Microsystems Technology Laboratories.

It seems like that should be easy, but it isnt, he said. There are other ways of accomplishing the same kind of cell separation, but they require complex and expensive equipment, or are limited in the number of cells they can process.

The new system uses a simple transparent silicone layer bonded to a conventional glass microscope slide. Fabricated in the layer are a series of tiny cavities, or traps, in which cells settle out after being added to the slide in a solution. Up to 10,000 cells could be sorted on a single slide.

Looking through the microscope, either a technician or a computerized system can check each cell to determine whether it has fluorescence in the right area or at the right time to meet the selection criteria. If so, its position is noted by the computer. At the end of the selection process, all of the cells whose positions were recorded are then levitated out of their traps using the pressure of a beam of targeted light from a low-cost laser. A flowing fluid then sweeps the selected cells off to a separate reservoir.

The laser levitation of the cells acts like a fire hose pushing up a beach ball, Voldman says. But the laser method is gentle enough that the living cells remain viable after the process is complete, allowing further biological testing.

Voldman and Kovac are continuing to refine the system, working on making it easier to use and on improving its ability to keep samples sterile. Voldman says that unlike expensive separation techniques such as optical tweezers, the new system could cost only a few thousand dollars. As a result, it could be employed in a variety of biological research laboratories or clinical settings, not just in big, centralized testing facilities.


Contact: Elizabeth Thomson
Massachusetts Institute of Technology

Related biology news :

1. ESF EURYI award winner aims to stop cancer cells reading their own DNA
2. Newly created cancer stem cells could aid breast cancer research
3. AIDS interferes with stem cells in the brain
4. Clemson scientists shed light on molecules in living cells
5. Social habits of cells may hold key to fighting diseases
6. UF scientists reveal how dietary restriction cleans cells
7. Human derived stem cells can repair rat hearts damaged by heart attack
8. Scientists identify embryonic stem cells by appearance alone
9. Cells united against cancer
10. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
11. U of M begins nations first clinical trial using T-reg cells from cord blood in leukemia treatment
Post Your Comments:
(Date:10/29/2015)... ANN ARBOR, Mich. , Oct. 29, 2015 ... with Eurofins Genomics for U.S. distribution of its ... DNA-seq kit and Rubicon,s new ThruPLEX Plasma-seq ... DNA to enable the preparation of NGS libraries ... in plasma for diagnostic and prognostic applications in ...
(Date:10/27/2015)... , Oct. 27, 2015 In the ... issues of concern for various industry verticals such as ... due to the growing demand for secure & simplified ... various ,sectors, such as hacking of bank accounts, misuse ... electronic equipment such as PC,s, laptops, and smartphones are ...
(Date:10/26/2015)... LAS VEGAS , Oct. 26, 2015 ... an innovator in modern authentication and a founding member ... launch of its latest version of the Nok Nok™ ... to use standards-based authentication that supports existing and emerging ... Suite is ideal for organizations deploying customer-facing applications that ...
Breaking Biology News(10 mins):
(Date:12/1/2015)... SAN FRANCISCO , Dec. 1, 2015  Twist Bioscience, ... CEO, Emily Leproust , Ph.D., has been selected as ... of 2015 for fast-tracking the building blocks of life ... Global Thinkers whose contributions and work have changed ... --> "It is an honor to be ...
(Date:12/1/2015)... ALEXANDRIA, Va. , Dec. 1, 2015 ... provides senior debt to life sciences and healthcare services ... senior secured term loan with MDRejuvena, Inc. ("the Company"). ... commercialization and continued development of the Company,s Rejuvaphyl™ and ... --> Rejuvaphyl is the MDRejuvena brand of high ...
(Date:12/1/2015)... Dec. 01, 2015 ... of the "2016 Europe Cell Surface ... Technologies, Competitive Strategies, Opportunities for Suppliers--France, Germany, ... offering. --> ) has ... Europe Cell Surface Markers: Country Volume and ...
(Date:12/1/2015)... ... December 01, 2015 , ... Park Systems , ... add-on scanning ion conductance microscopy module to Park NX10 that is the only ... Park SICM benefits virtually all materials characterization that require measurements in liquid such ...
Breaking Biology Technology: