Navigation Links
MIT: New cell measurement system
Date:4/11/2010

CAMBRIDGE, Mass. Using a sensor that weighs cells with unprecedented precision, MIT and Harvard researchers have measured the rate at which single cells accumulate mass a feat that could shed light on how cells control their growth and why those controls fail in cancer cells.

The research team, led by Scott Manalis, MIT associate professor of biological engineering, revealed that individual cells vary greatly in their growth rates, and also found evidence that cells grow exponentially (meaning they grow faster as they become larger).

The new measurement system, reported in the April 11 edition of the journal Nature Methods, is the first technique that can measure cells' mass as they grow over a period of time, ranging from five to 30 minutes. Previous methods for measuring cell growth rates have focused on volume or length measurements, and have not yet exhibited the necessary precision for revealing single cell growth models.

How they did it: The cell-mass sensor, which Manalis first demonstrated in 2007, consists of a fluid-filled microchannel etched in a tiny silicon slab that vibrates inside a vacuum. As cells flow through the channel, one at a time, their mass slightly alters the slab's vibration frequency. The mass of the cell can be calculated from that change in frequency, with a resolution as low as a femtogram (10-15 grams).

Michel Godin, a former postdoctoral associate in Manalis' lab and co-lead author of the paper, developed a way to trap a cell within the microchannel by precisely coordinating the flow direction. That enables the researchers to repeatedly pass a single cell through the channel every second or so, measuring it each time it moves through.

The researchers studied four types of cells: two strains of bacteria (E. coli and B. subtilis), a strain of yeast and mammalian lymphoblasts (precursors to white blood cells). They showed that B. subtilis cells appear to grow exponentially, but they did not obtain conclusive evidence for E. coli. That's because there is so much variation between individual cell growth rates in E. coli, even for cells of similar mass, says Francisco Delgado, a grad student in Manalis' lab and co-lead author of the paper.

If cells do grow exponentially, it means there must be some kind of mechanism to control that growth. Otherwise, when cells divide into two slightly different-sized daughter cells, as they often do, the larger cell in each generation would always grow faster than the smaller cell, leading to inconsistent cell sizes.

"If there were no control over the process, the variation in cell size would be all over the map," says Marc Kirschner, professor of systems biology at Harvard Medical School and an author of the paper. However, biologists don't know yet how that control mechanism might work.

Next steps: In their current studies, the researchers are tagging proteins inside the cell with fluorescent molecules that reveal what stage of the cell cycle the cell is in, allowing them to correlate cell size with cell cycle position. They are also working on a way to add chemicals such as nutrients, antibiotics and cancer drugs to the fluid inside the microchannel, so they can study how those substances affect growth rates.


'/>"/>

Contact: Jennifer Hirsch
jfhirsch@mit.edu
617-253-1682
Massachusetts Institute of Technology
Source:Eurekalert

Related biology news :

1. Innerscope Research Adds to Its Advisory Board Two Pioneers in the Measurement and Application of Emotional Engagement
2. NIST issues human milk and blood serum SRMs for contaminant measurements
3. Innerscope Research Adds to Its Advisory Board Two Pioneers in the Measurement and Application of Emotional Engagement
4. Carbon accounting from atmospheric measurements -- the aircraft perspective
5. UI researchers help to improve carbon measurements in global climate studies
6. Successful series of measurements in Arctic sea ice
7. Scientists demonstrate the sharpest measurement of ice crystals in clouds
8. LDL particle measurement by NMR recognized by ADA, ACC
9. Worldwide atmospheric measurements will determine the role of atmospheric fine particles
10. Measurement Specialties, Inc. Announces Impact of German Business Tax Reform 2008 Resulting in Lower Combined German Tax Rate - Impacts 2Q08 Reported Financial Results
11. Unprecedented global measurement network achieves full coverage of oceans
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/7/2016)... , June 7, 2016  Syngrafii Inc. and ... business relationship that includes integrating Syngrafii,s patented LongPen™ ... project. This collaboration will result in greater convenience ... credit union, while maintaining existing document workflow and ... ...
(Date:6/1/2016)... 1, 2016 Favorable Government Initiatives ... and Criminal Identification to Boost Global Biometrics System Market ... TechSci Research report, " Global Biometrics Market By ... and Opportunities, 2011 - 2021", the global biometrics market ... on account of growing security concerns across various end ...
(Date:5/9/2016)... Elevay is currently known as ... for high net worth professionals seeking travel for work ... world, there is still no substitute for a face-to-face ... your deal with a firm handshake. This is why ... of citizenship via investment programs like those offered by ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... , June 27, 2016  Sequenom, Inc. (NASDAQ: ... to enabling healthier lives through the development of innovative ... of the United States denied ... that the claims of Sequenom,s U.S. Patent No. 6,258,540 ... eligibility criteria established by the Supreme Court,s Mayo Collaborative ...
(Date:6/27/2016)... ... June 27, 2016 , ... Parallel 6 , the leading software ... Clinical Reach Virtual Patient Encounter CONSULT module which enables both audio and ... clinical trial team. , Using the CONSULT module, patients and physicians can schedule a ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, former senior ... the University of North Carolina Kenan-Flagler Business School effective June 27. ... Kenan-Flagler, with a focus on the school’s international efforts, leading classes and participating ...
(Date:6/27/2016)... BOSTON , June 27, 2016   Ginkgo ... biology to industrial engineering, was today awarded as ... a selection of the world,s most innovative companies. ... at scale for the real world in the ... organism engineers work directly with customers including Fortune ...
Breaking Biology Technology: