Navigation Links
MDC scientists show how hematopoietic stem cell development is regulated
Date:10/6/2009

During cell division, whether hematopoietic stem cells (HSCs) will develop into new stem cells (self-renewal) or differentiate into other blood cells depends on a chemical process called DNA methylation. These were the findings of researchers at the laboratory of Dr. Frank Rosenbauer of the Max Delbrck Center for Molecular Medicine (MDC) Berlin-Buch in cooperation with the laboratory of Professor Sten Eirik W. Jacobsen (Lund University, Sweden and the University of Oxford, England). Furthermore, the researchers showed that DNA methylation also plays a crucial role for cancer stem cells (Nature Genetics, online, doi: doi:10.1038/ng.463)*.

A group of three enzymes, the DNA methyltransferases (Dnmt) regulates the addition of methyl groups to the DNA (DNA methylation). One of these enzymes Dnmt1 is responsible for the maintenance of the marks with the methyl groups, the DNA methylation pattern, because the distribution of the methyl groups on the DNA decides which genes are transcribed and which are blocked. Researchers speak in this context of epigenetic information, in contrast to genetic information.

However, it was unclear until now whether DNA methylation plays a special role in the control of hematopoietic stem cell characteristics. From the HSCs all of the blood cells of the body are formed. Since blood cells have only a limited lifetime, the body must form new blood cells over and over again. The pool for this is generated by the HSCs.

In order to discover what function DNA methylation has for HSCs, the two doctoral students Ann-Marie Brske and Lena Vockentanz of the MDC research laboratory of Dr. Rosenbauer switched off the enzyme Dnmt1 in the mice. As a result, the animals were not viable because the hematopoietic stem cell function was completely disturbed.

By contrast, when the two researchers arranged that the HSCs formed just a little Dnmt1, the animals survived, but the HSCs lost their potential for self-renewal. Moreover, the HSCs were restricted in their formation of B cells and T cells (blood cells of the lymphatic system and important cells of the immune system).

However, the HSCs were able to form red blood cells, which are important for oxygen transport and belong to the blood cells of the myeloerythroid system. In other words, the DNA methylation level regulates which blood cell lineages develop or not from a hematopoietic stem cell.

Cancer stem cells

Methylation processes also play a role in numerous cancer diseases. As the MDC researchers were able to show, the DNA methylation by the enzyme Dnmt1 also controls the development of leukemic stem cells.

If the DNA methylation level is low, cancer stem cell renewal is restricted. Moreover, the formation of leukemic cells of B-cell lineage (acute B-cell leukemia ALL) is blocked.

The question is whether diseased stem cells can be switched off, possibly through a blockade of the enzyme Dnmt1. Dr. Rosenbauer and his research team want to make a more detailed investigation of this question in a further project.


'/>"/>

Contact: Barbara Bachtler
bachtler@mdc-berlin.de
49-309-406-3896
Helmholtz Association of German Research Centres
Source:Eurekalert

Related biology news :

1. UK scientists working to help cut ID theft
2. Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes
3. Comet probes reveal evidence of origin of life, scientists claim
4. Scientists link fragile X tremor/ataxia syndrome to binding protein in RNA
5. Male elephants get photo IDs from scientists
6. Scientists retrace evolution with first atomic structure of an ancient protein
7. Muscle mass: Scientists identify novel mode of transcriptional regulation during myogenesis
8. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
9. Clemson scientists shed light on molecules in living cells
10. Scientists tackle mystery mountain illness
11. T. rex quicker than Becks, say scientists
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
(Date:3/30/2017)... 30, 2017 Trends, opportunities and forecast in ... by technology (fingerprint, AFIS, iris recognition, facial recognition, hand ... by end use industry (government and law enforcement, commercial ... banking, and others), and by region ( North ... Asia Pacific , and the Rest of ...
(Date:3/27/2017)... ROCKVILLE CENTRE, N.Y. , March 27, 2017 ... by Healthcare Information and Management Systems Society (HIMSS) ... Analytics Outpatient EMR Adoption Model sm . In ... top 12% of U.S. hospitals using an electronic ... recognized CHS for its high level of EMR ...
Breaking Biology News(10 mins):
(Date:8/23/2017)... ... , ... Having proven that its Disruptor™ technology is more ... Green Cell Technologies® (GCT®), has turned its attention to the lucrative fruit ... methods, ushering in a new era of modern, cost effective and more natural ...
(Date:8/22/2017)... ... 22, 2017 , ... Cutting Edge Spine , a ... spinal implant technologies, announced today the appointment of Luke Maher as Director U.S. ... experience to directing Cutting Edge Spine’s national sales force, including 12+ years at ...
(Date:8/21/2017)... Kamuela/Waimea (PRWEB) , ... August 21, 2017 , ... ... based on the Big Island of Hawaii, is pleased to announce the first ... Trudeau, R(S) of MacArthur Sotheby’s International Realty is working together with listing agent ...
(Date:8/21/2017)... , ... August 21, 2017 , ... ... two speakers for this two-part educational webinar, in which attendees will learn about ... associated protein factor composition. Along with an overview of the development and validation ...
Breaking Biology Technology: