Navigation Links
Low-cost, ultra-fast DNA sequencing brings diagnostic use closer
Date:5/19/2010

BOSTON (5-19-10) -- Sequencing DNA could get a lot faster and cheaper and thus closer to routine use in clinical diagnostics thanks to a new method developed by a research team based at Boston University. The team has demonstrated the first use of solid state nanopores tiny holes in silicon chips that detect DNA molecules as they pass through the pore to read the identity of the four nucleotides that encode each DNA molecule. In addition, the researchers have shown the viability of a novel, more efficient method to detect single DNA molecules in nanopores.

"We have employed, for the first time, an optically-based method for DNA sequence readout combined with the nanopore system," said Boston University biomedical engineer Amit Meller, who collaborated with other researchers at Boston University, and at the University of Massachusetts Medical School in Worcester. "This allows us to probe multiple pores simultaneously using a single fast digital camera. Thus our method can be scaled up vastly, allowing us to obtain unprecedented DNA sequencing throughput."

The research is detailed in Nano Letters [http://pubs.acs.org/doi/abs/10.1021/nl1012147]. The National Institutes of Health are currently considering a four-year grant application to further advance Meller's nanopore sequencing project.

This low-cost, ultra-fast DNA sequencing could revolutionize both healthcare and biomedical research, and lead to major advances in drug development, preventative medicine and personalized medicine. By gaining access to the entire sequence of a patient's genome, a physician could determine the probability of that patient developing a specific genetic disease.

The team's findings show that nanopores, which can analyze extremely long DNA molecules with superior precision, are uniquely positioned to compete with current, third-generation DNA sequencing methods for cost, speed and accuracy. Unlike those approaches, the new nanopore method does not rely on enzymes whose activity limits the rate at which DNA sequences can be read.

"This puts us in the unique advantageous position of being able to claim that our sequencing method is as fast as the rapidly evolving photographic technologies," said Meller. "We currently have the capability of reading out about 200 bases per second, which is already much faster than other commercial third-generation methods. This is only the starting point for us, and we expect to increase this rate by up to a factor of four in the next year." Licensing intellectual property from Boston University and Harvard University, Meller and his collaborators recently founded NobleGen Biosciences to develop and commercialize nanopore sequencing based on the new method.

"I believe that it will take three to five years to bring cheap DNA sequencing to the medical marketplace, assuming an aggressive research and development program is in place," said Meller.


'/>"/>

Contact: Michael Seele
mseele@bu.edu
617-353-9766
Boston University College of Engineering
Source:Eurekalert

Related biology news :

1. CU physicists use ultra-fast lasers to open doors to new technologies unheard of just years ago
2. Sequencing of first frog genome sheds light on treating disease
3. New Stanford tool enables wider analyses of genome deep sequencing
4. First whole genome sequencing of family of 4 reveals new genetic power
5. Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca)
6. Genomatix gives a hand in the analysis of Wayne States sequencing projects
7. Life Technologies, TGen and US Oncology partner on groundbreaking breast cancer sequencing research
8. DNA sequencing unlocks relationships among flowering plants
9. Successful genome sequencing of pea aphid is a breakthrough for ecology and agricultural research
10. Genome sequencing of 3 parasitoid wasp species
11. ASU scientists develop universal DNA reader to advance faster, cheaper sequencing efforts
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/6/2017)... Forecasts by Product Type (EAC), ... End-Use (Transportation & Logistics, Government & Public Sector, Utilities ... Generation Facility, Nuclear Power), Industrial, Retail, Business Organisation (BFSI), ... you looking for a definitive report on the $27.9bn ... ...
(Date:4/3/2017)... WASHINGTON , April 3, 2017 /PRNewswire-USNewswire/ ... single-cell precision engineering platform, detected a statistically ... cell product prior to treatment and objective ... highlight the potential to predict whether cancer ... prior to treatment, as well as to ...
(Date:3/28/2017)... 28, 2017 The report "Video ... Monitors, Servers, Storage Devices), Software (Video Analytics, VMS), and ... Global Forecast to 2022", published by MarketsandMarkets, the market ... is projected to reach USD 75.64 Billion by 2022, ... The base year considered for the study is 2016 ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... The Blavatnik Family ... and six Finalists of the 2017 Blavatnik Regional Awards for Young Scientists. ... and administered by the New York Academy of Sciences to honor the excellence ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... the first-ever genomics analysis platform specifically designed for life science researchers to ... of pioneering researcher Rosalind Franklin, who made a major contribution to the ...
(Date:10/11/2017)... ... October 11, 2017 , ... Proscia ... be hosting a Webinar titled, “Pathology is going digital. Is your lab ready?” ... pathology adoption best practices and how Proscia improves lab economics and realizes an ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... has granted orphan drug designation to SBT-100, its novel anti-STAT3 (Signal Transducer and ... of osteosarcoma. SBT-100 is able to cross the cell membrane and bind intracellular ...
Breaking Biology Technology: