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Looking for the heartbeat of cellular networks
Date:12/16/2009

xcited with a stroboscopic lamp and a CCD camera registered time and amplitude of the fluorescence, thus visualizing the concentration alterations with a spatial resolution of about 500 nanometres. The experiments revealed that DNA-strands comprising 16 units, the so-called bases, showed a sevenfold higher reaction speed compared to values determined outside living cells.

12-base DNA-strands, on the other hand, reacted times five times slower than outside cells. This is a surprising result, since kinetics of molecular reactions has been assumed to be always faster inside cells, where much higher molecular concentrations prevail. "Apparently cells modulate the reaction speed in a highly selective way," says Braun. "The measurements provide valuable insight into in vivo kinetic data for the systematic analysis of the complexity of biological cells," adds Ingmar Schn, who conducted the demanding experiments. The scientists are now planning to probe a wide variety of molecular reactions in living cells, visualizing the heartbeat of cellular networks. (CR/suwe)


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Contact: Professor Dieter Braun
dieter.braun@lmu.de
49-892-180-2317
Ludwig-Maximilians-Universitt Mnchen
Source:Eurekalert

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