Navigation Links
Looking for the heartbeat of cellular networks
Date:12/16/2009

Our cells' molecules form an intricate network of interactions. Today's techniques, however, can only be used to measure individual molecular reactions outside the cells. Since molecular concentrations are much higher in cells than in the laboratory, scientists suspect that the kinetics of molecular reactions in living cells differ substantially from external probes. We expected the cellular reaction speed to be higher," confirms LMU biophysicist Professor Dieter Braun. "However, our novel optical approach showed that depending on the length of the strands the coupling of DNA-strands inside living cells can be both faster and slower than outside." Data yielded from living cells are highly valuable for the development of models to understand the complex interactions as well as pathological processes in biological cells. Braun and his team now plan to probe a variety of molecular reactions in living cells, visualizing the heartbeat of cellular networks. (PNAS online, 14 November 2009)

In their work, the scientists investigated the hybridization the coupling and de-coupling of two DNA-strands, which they introduced into living cells. To determine the reaction time constant they used an infrared laser to induce temperature oscillations of different frequencies in the cell and measured the concentration of the reaction partners, namely of coupled and de-coupled DNA. At low frequencies, these concentrations followed the temperature oscillations, whereas at higher frequencies they experienced a phase delay and oscillated with diminished amplitude. Both delay time and amplitude decrease, were evaluated to obtain the reaction time constant.

The team determined the concentrations using the so-called fluorescent energy transfer (FRET), which takes place between two chromophores at a certain spatial distance. They applied a FRET pair to the DNA-strands such that energy transfer occurred only if the strands were coupled. The chromophores were e
'/>"/>

Contact: Professor Dieter Braun
dieter.braun@lmu.de
49-892-180-2317
Ludwig-Maximilians-Universitt Mnchen
Source:Eurekalert

Page: 1 2

Related biology news :

1. Iowa State researchers looking for catalyst that allows plants to produce hydrocarbons
2. UAB researchers looking for genetic predictors for suicide
3. Case Western Reserve researchers looking at light-induced toxins in air and water
4. New satellite techniques for looking at climate change
5. Normal-looking sperm may have serious damage; scientists urge more care in selection
6. Looking for the Founatain of Youth? Cut your calories, research suggests
7. Are you looking at me?
8. Looking at neurons from all sides
9. Looking through the eyes of a mouse, scientists monitor circulating cells in its bloodstream
10. Carnegie Mellon researchers to develop probes to study cellular GPS
11. Think what you eat: Studies point to cellular factors linking diet and behavior
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/10/2014)... NEW YORK , Dec. 8, 2014 You,ve ... online banking account but can,t remember your password, site key ... birthday? Who was your first grade teacher? ... launches the app that will finally put an ... PINs – 1U TM . 1U leverages a ...
(Date:12/10/2014)... Forest Baptist Medical Center today announced plans for a ... Funding for this $50 million capital project is part ... launched next summer. The medical education ... R.J. Reynolds Tobacco Company complex, adjacent to 525@vine in ... plans to be ready to welcome medical students in ...
(Date:12/10/2014)... --Research and Markets ( http://www.researchandmarkets.com/research/xclcvw/biometrics_market ) ... Market in Japan 2014-2018" report to their ... The integration of biometrics and smart ... upgradation of the driver,s license is one of ... the aforementioned projects, biometrics is being used as ...
Breaking Biology News(10 mins):The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... in Spanish . , Due to the large ... have claimed that they are millennia old but "there had never ... by the ecologist Bernat Claramunt from the Centre for Ecological Research ... has now analysed the ages of the famous olive trees and ...
... Fla. (May 16, 2012) Chlorothalonil, one of ... crops and golf courses, was lethal to a wide ... of South Florida researchers said Wednesday. Biologists Taegan ... in the journal Ecology Letters , report that ...
... leading event in reproductive medicine is less than two months ... journalism - and ESHRE,s annual meeting one of the best ... Istanbul promises to be as scientifically and clinically strong as ... in reproduction are expected this year. More than 1700 abstracts ...
Cached Biology News:The millennium-old olive trees of the Iberian Peninsula are younger than expected 2USF study: Common fungicide wreaks havoc on freshwater ecosystems 2European Society of Human Reproduction and Embryology 28th Annual Meeting -- Istanbul 2
(Date:12/13/2014)... /CNW/ - MaRS and Virgin Unite Canada, the non-profit foundation ... forces to support Canadian entrepreneurs tackling social and environmental challenges. ... at the MaRS Centre to announce the new partnership, which ... This fund has $1 million in seed capital provided by ... Mindset Social Innovation Foundation, founded by Alison Lawton . ...
(Date:12/13/2014)... The DNA Microarray market market ... is expected to grow at a CAGR of ... the TOC of the DNA Microarray for an ... with help of various tables and figures. , ... market consists of instrument, consumables, and services, where ...
(Date:12/13/2014)... The “Global Synthetic Biology Market (2013-2018)”, analyses and ... challenges. , The global synthetic biology market is ... Scientific, Inc. (U.S.), DuPont (U.S.), and Royal DSM ... ~65% of the total synthetic biology market. ... @ http://www.marketsandmarkets.com/Market-Reports/synthetic-biology-market-889.html , Thermo Fisher Scientific, ...
(Date:12/13/2014)... QuickSTAT is making significant investments in ... capabilities, and is delighted to announce the opening of ... Life Science clients in the region. , QuickSTAT France, ... will provide 24/7 transportation and logistics services, as well ... specimens, API, following IATA rules and EU GDP guidelines. ...
Breaking Biology Technology:MaRS and Virgin Unite partner to invest in business as a force for good 2MaRS and Virgin Unite partner to invest in business as a force for good 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4Synthetic Biology Market to HIt $5.6304 Billion by 2018 - New Research Report by MarketsandMakets 2Synthetic Biology Market to HIt $5.6304 Billion by 2018 - New Research Report by MarketsandMakets 3Quick Opens Office in France to Serve Life Science Community In France and Neighboring French-Speaking Countries 2
... by Commerce Sec. Cory Nettles to talk about aiding ... of Commerce will fund a public-sector coalition to provide ... around the state, Governor Jim Doyle announced on Monday. ... for five years, he said. Commerce expects another $400,000 ...
... enact and implement all of the great ideas floating around ... picture of what that economy will look like in the ... of what needs to be accomplished. Capping the growth of ... broadband adoption, linking the colleges and universities with the business ...
... be allowed to tax Internet usage, in a move that ... hole in the state budget. , ,The federal measure to ... , R-Wis., who said home users could save around a ... Tax Nondiscrimination Act, a tech-neutral plan that exempts all Internet ...
Cached Biology Technology:State and university form network for entrepreneurial outreach 2State and university form network for entrepreneurial outreach 3Wisconsin 2020: A vision from a state senator 2Wisconsin 2020: A vision from a state senator 3State faces revenue drop as federal Internet tax ban passes House 2
Phospho-FAK (Tyr576/577) Antibody...
Latanoprost EIA Antiserum EIAs neutrophils antisera enzyme immunoassays reagents...
Rabbit polyclonal to ZFP200 ( Abpromise for all tested applications). Antigen: Synthetic peptide: CGKSFNHKTNLNKHER (Human) Entrez Gene ID: 7752 Swiss Protein ID: P98182...
Human Ig Lambda-chain, clone ICO-16, Monoclonal Antibody...
Biology Products: