Navigation Links
Long-term use of mechanical ventilation contributes to the deterioration of human diaphragm muscle
Date:3/26/2008

PHILADELPHIA A new study by University of Pennsylvania School of Medicine shows, for the first time in humans, that ventilators combined with diaphragm disuse contributes to muscle atrophy in the diaphragm in as little as eighteen hours. Muscle atrophy in the diaphragm is a major contributor of why patients who have undergone prolonged mechanical ventilation often have difficulty breathing after being removed from the ventilator.

The report, published in the March 27th edition of the New England Journal of Medicine, measured a greater than 50 percent decline in muscle fibers in the diaphragm. In addition, the study measured the proteins that play a key role in the muscle-wasting chemical cascade. By intervening in this pathway, the research suggests, a new pharmacological approach to safely and quickly wean patients off ventilators could be developed.

Sanford Levine, PhD, Professor of Thoracic Surgery and co-director of the Respiratory Muscle Research Laboratory, led a team that conducted 22 biopsies on both deceased and living patients. Fourteen brain-dead organ donors, aged 18 to 58, comprised the case study with each having undergone between 18 and 69 hours of mechanical ventilation. The eight-member control group each received less than three hours.

Levine said both groups were demographically and statistically similar except for the time each had spent on mechanical ventilation. Biopsy results on the other hand were different.

Compared to the control group, the diaphragms of the 14 case study members revealed:

  • 23 percent lower levels of the free-radical fighting antioxidant, glutathione

  • 154 percent more Caspase-3, a calcium-dependant executioner enzyme that degrades protein molecules and is responsible for programmed cell deaths

  • Muscle-wasting Atrogin-1 genes at a ratio of 200:1 over MBD4 housekeeper genes that maintain healthy cells

  • MURF-1 nucleotides that attack myofiber proteins at a ratio of 590:1 over housekeeper genes

From our observations, we conclude that these (biopsy differences) could only be attributed to marked atrophy caused by a combination of complete diaphragm inactivity and mechanical ventilation, Levine said.

Disuse atrophy of human diaphragm myofibers could be a major contributor to the weaning problems that occur in some of our patients, Levine said. Therefore, we believe fiber atrophy of the magnitude noted in our case diaphragms could have clinical significance.


'/>"/>

Contact: Marc Kaplan
Marc.Kaplan@uphs.upenn.edu
215-662-2560
University of Pennsylvania School of Medicine
Source:Eurekalert

Related biology news :

1. Novel mechanism for long-term learning identified by Carnegie Mellon researchers
2. Atomic bomb survivor, scientists mark 60 years of long-term radiation study
3. Tropical crab invades Georgia oyster reefs -- but the long-term impact cant be predicted
4. Meth exposure in young adults leads to long-term behavioral consequences
5. Videos extract mechanical properties of liquid-gel interfaces
6. New mechanical insights into wound healing and scar tissue formation
7. Improving detection of nuclear smuggling goal of computer model of mechanical engineer
8. 4 days of REM sleep deprivation contributes to a reduction of cell proliferation in rats
9. KGI professor contributes new insights on jumping genes
10. Scientists launch first comprehensive database of human oral microbiome
11. Scientists launch human oral microbiome database
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/28/2017)... Germany , February 28, 2017 News ... ... Amsterdam from 14 to 16 March, Materna ... destination, and show how seamless travel is a real benefit for ... has added biometrics to their passenger touch point solutions to take ...
(Date:2/22/2017)... 2017 With the biometrics market to ... four technologies that innovative and agile startups must ... in the changing competitive landscape: multifactor authentication (MFA), ... "Companies can no longer afford to ... Dimitrios Pavlakis , Industry Analyst at ABI ...
(Date:2/13/2017)... , Feb. 13, 2017  RSA Conference -- ... platform that is designed to enhance fraud detection ... release in the RSA Fraud & Risk Intelligence ... organizations to leverage additional insights from internal and ... to better protect their customers from targeted cybercrime ...
Breaking Biology News(10 mins):
(Date:3/24/2017)... and ROCKVILLE, Md. , March ... of Maxwell Biotech Venture Fund (MBVF), today announced positive ... added to the standard drug therapy regimen in patients ... small molecule drug discovered by scientists at Sequella, Inc. ... Institutes of Health. A total of ...
(Date:3/24/2017)... 2017 Agenus Inc. (NASDAQ: AGEN), an immuno-oncology ... cancer vaccines, today announced participation at the following conferences: ... Blair and Maidstone Life Sciences conference "Cancer Immunotherapy Conference" ... New York, NY . Agenus will participate ... 9:40 am: Robert B. Stein , M.D., Ph.D., ...
(Date:3/23/2017)... 23, 2017  SeraCare Life Sciences, Inc., a ... vitro diagnostics manufacturers and clinical laboratories, is ... multiplexed Inherited Cancer reference material ... next-generation sequencing (NGS). The Seraseqâ„¢ Inherited Cancer DNA ... from industry experts to validate the ability ...
(Date:3/23/2017)... YORK , March 23, 2017 ... ... of death, putting significant strain on health care systems, in ... cancer diagnoses rises, so too does the development of innovative ... minimum side effects. Among the many types of cancer treatments, ...
Breaking Biology Technology: