Navigation Links
Lollipops and ice fishing: Molecular rulers used to probe nanopores
Date:4/28/2010

de of the membrane to the pinch point, and in turn, the entire length of the nanopore by adding the two measurements together. The probes consist of DNA strands of known lengths topped on one end by a polymer sphere. The sphere prevents the probe from completely moving through the nanopore while leaving the DNA chain dangling from it free to extend into the channel. If the chain reaches the pinch point, the force that would normally drive a free DNA chain past the junction instead holds the probe in place (since the polymer sphere "locks" it at the other end) and defines the distance to the pinch point. If the chain is shorter than the distance to the pinch point, it will be bounced out of the nanopore, telling researchers that a longer-length chain is needed to measure the distance to the gap.

The NIST/Maryland researchers also developed a second means of measuring the length of the nanopore to confirm the results of the "single lollipop" method. In this system, polymer molecules are allowed to circulate freely in the solution found on the inner side of the membrane. Polymer-capped DNA probes of different lengths are forced one at a time into the nanopore from the opposite side. If the end of a probe's chain is long enough to completely transverse the channel, it will grab hold of a free polymer molecule in solution. This defines the length of the channel.

Additionally, this "ice fishing" method provides insight into the structure of the nanopore. As the DNA chain winds its way through, changes in electrical voltage correspond to the changing shape of the channel. This information can be used to effectively map the passageway.


'/>"/>

Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Page: 1 2

Related biology news :

1. New molecular regulators of hyperthyroidism and goiter
2. Carnegie Mellon scientists investigate initial molecular mechanism that triggers neuronal firing
3. UC health news: molecular pathway may predict chemotherapy effectiveness
4. New molecular clock from LLNL and CDC indicates smallpox evolved earlier than believed
5. Story ideas from Molecular & Cellular Proteomics
6. Lets talk -- new paradigms in the research of the biomolecular composition of water
7. Scientists unveil structure of molecular target of many drugs
8. Potential new therapeutic molecular target to fight cancer
9. NIH selects LIAI for major study on allergy molecular causes and possible treatments
10. Pennsylvania Hospital surgeon receives grant to develop molecular cardiac surgery
11. Leading cause of death in preemies might be controlled by resetting a molecular switch
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Lollipops and ice fishing: Molecular rulers used to probe nanopores
(Date:4/23/2014)... coal for domestic heating may contribute to early fetal ... The Saban Research Institute of Children,s Hospital Los Angeles ... the world. , In a paper published today in ... report ,"alarmingly strong statistical correlations" between seasonal ambient air ... , UB has one of the highest levels of ...
(Date:4/23/2014)... in French and Spanish . ... 2014Today, the CEC released its Conservation Assessment for the ... Conservation, which identifies 29 priority conservation areas in a ... different protected areas in the states of Texas, Coahuila, ... and semi-arid habitats inhabited by rare and endangered plants ...
(Date:4/23/2014)... accurate group decision increases with the abundance of brains ... a variety of factors as often happens in ... smaller groups actually tend to make more accurate decisions ... certain pieces of information. , The findings present a ... or the "wisdom of crowds," wherein individual observations ...
Breaking Biology News(10 mins):Pollutants from coal-burning stoves strongly associated with miscarriages in Mongolia 2Conservation priorities released for several protected areas along US-Mexico border 2Conservation priorities released for several protected areas along US-Mexico border 3Too many chefs: Smaller groups exhibit more accurate decision-making 2Too many chefs: Smaller groups exhibit more accurate decision-making 3
... mother,s diet during gestation and lactation were found to be ... those not exposed to the chemical, researchers at the University ... is a chemical most commonly found in the lining of ... many hard plastic bottles, including baby bottles, but many companies ...
... Conn.Like organisms, cities need energy, water, and nutrients, and ... ways that are viable and sustainable over the long ... for looking systematically at the resources that flow into ... from themto understand the environmental impacts of cities and ...
... 2013 UC Irvine neuroscientists have developed a way ... a novel opportunity to treat the most severe manifestations ... of temporal lobe epilepsy, Ivan Soltesz, Chancellor,s Professor and ... EEG-based computer system that activates hair-thin optical strands implanted ...
Cached Biology News:Female mice exposed to BPA by mothers show unexpected characteristics 2Female mice exposed to BPA by mothers show unexpected characteristics 3Urban metabolism for the urban century 2
(Date:1/15/2014)... FALLS, N.Y., Jan. 15, 2014   Niagara Thermal ... been selected by Buffalo BioBlower Technologies LLC ("B3") as ... SBIR Phase 2 award from the Army Corps of ... system that kills all biological contaminants and destroys VOCs ...
(Date:1/15/2014)... Cynvenio, a cancer diagnostics company focused ... analysis of tumor cells in the bloodstream, today announced ... decision support application powered by CollabRx, Inc. , ... test, Therapy Finder will provide breast cancer patients and ...
(Date:1/15/2014)... 2014 ­ RedBrick Health , a fast-growing leader ... announces that EmblemHealth , the New ... now providing the RedBrick Compass health assessment, combined with ... of its members. EmblemHealth is among the first health ...
(Date:1/15/2014)... 2014 A study has been launched to ... 1 track could help to tackle the problem of obesity. ... (a GP surgery based in Stowmarket) and academics at University ... Telemetry technology, which is inspired by equipment used to ...
Breaking Biology Technology:Niagara Thermal Products LLC Selected as Critical Supplier for Buffalo BioBlower Air Purification Systems 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4
... In the quest to exploit unique properties at ... have developed a novel technique for creating uniform ... and students in the Department of Physics and ... methods from holographic lithography to demonstrate a new ...
... Pharmaceuticals, Inc. (NASDAQ: IDIX ), announced today that ... Stanley Global Healthcare Conference on September 13 at 8:35 a.m. ... the Bank of America Merrill Lynch Global Healthcare Conference on ... of America Merrill Lynch Financial Centre, London.   ...
... Cellectis (Alternext: ALCLS), the French genome engineering specialist, will present at ... September 20, 2011, and at the MidCap Event in Paris on ... will present the activities and the development strategy of the Cellectis ... Hyatt New York hotel on Tuesday, September 20, 2011 at 11:30 ...
Cached Biology Technology:Stevens researchers pioneer novel technique to make plasmonic nanogap arrays 2Stevens researchers pioneer novel technique to make plasmonic nanogap arrays 3
RABBIT ANTI ISOCITRATE DEHYDROGENASE...
... two highly efficient methods of identifying recombinant ... By using the large-scale screen method in ... is to transfer partial colonies of E. ... into the solutions provided by the kits ...
Recombinant Rat Leptin, CF...
Rat whole blood (Sprague-Dawley, averaging 7-8 weeks of age, either sex) is collected at the time of slaughter and refrigerated within 6 hours, and processed and frozen within 24 hours. The serum is ...
Biology Products: