Navigation Links
Liver cells benefit from mesenchymal stem cell co-culture prior to transplantation
Date:12/18/2013

Putnam Valley, NY. (Dec. 18, 2013) Hepatocyte (liver cell) transplantation is becoming an accepted therapy for acute liver failure, either for liver regeneration or as a bridge to liver transplantation. However, maintaining the viability and functional aspects of hepatocytes has been a concern even with successful freezing (cryopreservation). In an effort to improve both the viability and function of cryopreserved hepatocytes prior to transplantation, researchers at Kings College Hospital, London, have co-cultured hepatocytes along with human mesenchymal stem cells (MSCs) derived from umbilical cord or fat tissues and found that the co-culturing confers improved hepatocyte survival and function.

The study will be published in a future issue of Cell Transplantation but is currently freely available on-line as an unedited early e-pub at: http://www.ingentaconnect.com/content/cog/ct/pre-prints/content-ct1038fitzpatrick.

The researchers hypothesized that when co-cultured with hepatocytes, MSCs - multipotent connective tissue cells that can differentiate into many kinds of cells - would lend pro-regenerative characteristics to hepatocytes.

"Both human umbilical cord and adipose tissue sources for MSCs were compared," said study corresponding author Dr. Emer Fitzpatrick of King's College Hospital. "Previous studies had used nonhuman cells with limited success. Human MSCs from both umbilical cords and adipose tissue have anti-apoptotic (anti-programmed cell death) and pro-regenerative effects on kidney failure and stroke and they are known to modulate liver injury and promote liver regeneration. While cryopreservation can have deleterious effects on hepatocyte cell engraftment, we wanted to investigate whether a co-culture of human MSCs and hepatocytes would improve hepatocyte function."

They found that MSCs and hepatocytes cultured together demonstrated an improved production of albumin from day five to day 25 of the culture.

The effect, they said, was most prominent at day 15 when albumin production was ten times that of a control monoculture. Urea production also improved in co-culture from day five to day 25. Once more, the viability of the co-cultured hepatocytes was superior to that of monocultured cells, with up to 16 percent improvement.

The researchers noted that MSCs are known to provide structural support for cells in the body and have anti-apoptotic and immunomodulatory effects and that the supportive role of MSCs is "particularly promising in the context of cell transplantation, allowing for sufficient time for an organ for transplant to become available."

"Our results suggest that the presence of the hepatocytes is necessary to stimulate the MSCs to secrete pro-survival factors," concluded the researchers. "The combination of transplanted hepatocytes and MSCs has great promise in the therapy of acute liver failure." "Mechanisms to stabilize hepatocyte cultures prior to transplantation for the treatment of liver diseases are a potentially important step in maximizing the potential of hepatocytes as a therapy" said Dr. Stephen Strom of the Karolinska Institute, Sweden and section editor for Cell Transplantation. "The use of human-derived stem cells as a support mechanism in culture is therefore of interest and further study will illuminate whether the pro-survival factors could be used alone or whether the cells themselves are required".


'/>"/>

Contact: Robert Miranda
cogcomm@aol.com
Cell Transplantation Center of Excellence for Aging and Brain Repair
Source:Eurekalert

Related biology news :

1. Nanotherapy: Treating deadly brain tumors by delivering big radiation with tiny tools
2. Crestor Delivers Latest LDL-C Targets in High-Risk Patients at Lower Doses than Other Statins
3. Researchers unravel genetic mechanism of fatty liver disease in obese children
4. Commonly used diabetes drug may help to prevent primary liver cancer
5. Ben-Gurion U. licenses drug delivery platform for central nervous system diseases to NY biotech firm
6. Gut microbiota transplantation may prevent development of diabetes and fatty liver disease
7. Gallbladder shown as potential stem cell source for regenerative liver and metabolic disease
8. Family history of liver cancer increases risk of developing the disease
9. Modest alcohol intake associated with less inflammation in patients with common liver disease
10. Delivery of gene-therapy for heart disease boosted 100-fold; now in 100-patient trial
11. Cancerous tumors deliver pro-metastatic information in secreted vesicles
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
(Date:3/29/2016)... Florida , March 29, 2016 ... the "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased ... in ink used in a variety of writing instruments, ... Buyers of originally created collectibles from athletes on LegacyXChange ... forensic analysis of the DNA. Bill ...
Breaking Biology News(10 mins):
(Date:5/4/2016)... , ... May 04, 2016 , ... PBI-Gordon Corporation is ... Agricultural Sales. , Doug began his career at PBI-Gordon in February 1988, after ... a wide variety of roles, ranging from customer service to national product manager, to ...
(Date:5/3/2016)... ... 03, 2016 , ... Morf Media Inc ., developer ... on mobile devices, today released a new interactive Food and Drug Administration ... course is essential for owners or operators of places of business that are ...
(Date:5/3/2016)... CO (PRWEB) , ... May 03, 2016 , ... ... , announced the addition of Dr. Nancy Gillett to its Board of Directors. ... position, she served as Corporate Executive Vice President and Chief Scientific Officer. A ...
(Date:5/3/2016)... LONDON , May 3, 2016 ... Report Assessing Developers and Producers of Those Competitor Biologics  ... Guide to Companies, Activities and Prospects ,  ... drug companies? And what are their sales potentials? ... There you see results, trends, opportunities and revenue forecasting. ...
Breaking Biology Technology: