Navigation Links
Listeria clever at finding its way into bloodstream, causing sickness

WEST LAFAYETTE, Ind. - Pathogenic listeria tricks intestinal cells into helping it pass through those cells to make people ill, and, if that doesn't work, the bacteria simply goes around the cells, according to a Purdue University study.

Arun Bhunia, a professor of food science, and Kristin Burkholder, a former Purdue graduate student who is now a postdoctoral researcher in microbiology and immunology at the University of Michigan Medical School, found that listeria, even in low doses, somehow triggers intestinal cells to express a new protein, heat shock protein 60, that acts as a receptor for listeria. This may allow the bacteria to enter the cells in the intestinal wall and exit into a person's bloodstream. Bhunia and Burkholder's findings were published in the early online version of the journal Infection and Immunity.

"It's possible that host cells generate more of these proteins in order to protect themselves during a stressful event such as infection," Burkholder said. "Our data suggest that listeria may benefit from this by actually using those proteins as receptors to enhance infection."

Listeria monocytogenes is a foodborne bacteria that can cause fever, muscle aches, nausea and diarrhea, as well as headaches, stiff neck, confusion, loss of balance and convulsions if it spreads to the nervous system. According to the U.S. Centers for Disease Control and Prevention, it sickens about 2,500 and kills 500 people each year in the United States and primarily affects pregnant women, newborns, older adults and those with weakened immune systems.

The findings suggest that listeria may pass between intestinal cells to sort of seep out of the intestines and into the bloodstream to cause infection.

"That can expedite the infection," Bhunia said.

Measurable increases of the heat shock 60 protein were detected when listeria was introduced to cultured intestinal cells.

Bhunia and Burkholder also introduced listeria to intestinal cells in the upper half of a dual-chamber container and counted the number of bacteria that passed through the cells and appeared in the lower chamber.

The bacteria moved to the lower chamber faster than it is known to do when moving through cells, and did so even when a mutant form of the bacteria that do not invade the intestinal cells was used. This suggests the bacteria are moving around the cells, Bhunia said.

"The infective dose is very low. Even 100 to 1,000 listeria cells can cause infection," Bhunia said. "We believe that these mechanisms are what allow listeria to cause infections at such low levels."

Bhunia said he next would try to understand how listeria and the heat shock 60 protein interact and work to develop methods to protect intestinal cells from the bacteria. The Center for Food Safety Engineering at Purdue funded part of the research.


Contact: Brian Wallheimer
Purdue University

Related biology news :

1. Honest crabs, power to the hungry, nice mice and clever meerkats: News from the American Naturalist
2. Unexpected findings of lead exposure may lead to treating blindness
3. Gynecologist disputes findings
4. New findings pull back curtain on relationship between iron and Alzheimers disease
5. MBL scientists reveal findings of World Ocean Microbe Census
6. Chromosomal break gives scientists a break in finding new puberty gene
7. Most complete beer proteome finding could lead to engineered brews
8. New VARI findings next step to growing drought-resistant plants
9. Findings overturn old theory of phytoplankton growth, raise concerns for ocean productivity
10. How the wrong genes are repressed: New finding from UCL
11. Scaffold gradients: Finding the right environment for developing cells
Post Your Comments:
Related Image:
Listeria clever at finding its way into bloodstream, causing sickness
(Date:11/17/2015)... , Nov. 17, 2015  Vigilant Solutions announces ... joined its Board of Directors. --> ... after recently retiring from the partnership at TPG Capital, ... companies with over $140 Billion in revenue.  He founded ... across all the TPG companies, from 1997 to 2013.  ...
(Date:11/12/2015)... CAMBRIDGE, Mass. , Nov. 12, 2015 /PRNewswire/ ... Broad Institute of MIT and Harvard for use ... chemical discovery information management tools. The partnership will ... share both biological and chemical research information internally ... tools will be used for managing the Institute,s ...
(Date:11/10/2015)... Nov. 10, 2015 About ... that helps to identify and verify the identity ... considered as the secure and accurate method of ... a particular individual because each individual,s signature is ... especially when dynamic signature of an individual is ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... SAN DIEGO , Nov. 24, 2015 Halozyme Therapeutics, ... Jaffray Healthcare Conference in New York on ... Dr. Helen Torley , president and CEO, will provide a ... New York at 1:00 p.m. ET/10:00 a.m. ... communication and investor relations, will provide a corporate overview. --> ...
(Date:11/24/2015)... ... 2015 , ... International Society for Pharmaceutical Engineering (ISPE) closed ... events for pharmaceutical manufacturing: 2015 Annual Meeting. The conference took place in Philadelphia, ... number of attendees in more than a decade. , “The 2015 Annual ...
(Date:11/24/2015)... India , November 24, 2015 ... a new market research report "Oligonucleotide Synthesis Market by ... Application (PCR, Gene Synthesis, Diagnostic, DNA, RNAi), End-User (Research, ... 2020", published by MarketsandMarkets, the market is expected to ... Million in 2015, at a CAGR of 10.1% during ...
(Date:11/24/2015)... Massachusetts , November 24, 2015 SHPG ... will participate in the Piper Jaffray 27 th Annual Healthcare ... Tuesday, December 1, 2015, at 8:30 a.m. EST (1:30 p.m. GMT). ... , Chief Financial Officer, will participate in the Piper Jaffray 27 ... , NY on Tuesday, December 1, 2015, at 8:30 a.m. ...
Breaking Biology Technology: