Navigation Links
Life's smallest motor, cargo carrier of the cells, moves like a seesaw
Date:2/18/2010

kinesin motors have enabled researchers to piece together the protein's three-dimensional structure. But these images don't reveal how it works.

"The problem is that it is not until the protein motor binds to a microtubule that structural rearrangements occur that enable ATP hydrolysis, the process that transfers energy from ATP to kinesin," says Downing.

To image kinesin at this critical stage, Downing and Sindelar turned to cryoelectron microscopy, which is a type of electron microscopy in which the sample is studied at extremely low temperatures. The technology is used by structural biologists to image proteins and other molecules as they appear in real-world conditions, in this case a kinesin protein attached to a microtubule.

The technique yielded 8 to 9 angstrom-resolution snapshots of the kinesin motor at four stages of the motor's cycle as it moves along a microtubule. One angstrom is one-ten billionth of a meter. Using these images as a guide, the researchers then "dropped in" even higher resolution crystallographic images of kinesin's components. This step enabled them to derive atomic-level structural models of kinesin in action.

"Collectively, this work provides a detailed molecular explanation for kinesin's microtubule-attached power stroke," says Downing. "In other words, we can see it how it works in real life. We looked at kinesin in different phases, and learned what causes it to move from one conformation to another, which is how it pulls cargo along the microtubule."

In addition to further elucidating a key biological process, Downing and Sindelar's research may inform the development of disease-fighting drugs. One of kinesin's main jobs is moving chromosomes apart during cell division. Anything that blocks this process will lead to cell death, which is the basis of several cancer therapies such as taxol.

"New insights into how kinesin works could allow scientists to develop drugs that target a
'/>"/>

Contact: Dan Krotz
dakrotz@lbl.gov
510-486-4019
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Lifestyle changes for teens critical in light of research about teens heart disease risk
2. Lifestyle changes may stave off diabetes for a decade
3. Genomes reveal bacterial lifestyles: Research
4. American College of Preventive Medicine releases lifestyle medicine literature review
5. Recruitment of reproductive features into other cell types may underlie extended lifespan in animals
6. Late motherhood boosts family lifespan
7. Modern lifestyle prevents tooth decay
8. M.I.N.D. Institute researchers call for fragile X testing throughout the lifespan
9. Sex and lifespan linked in worms
10. Scientists reveal the lifestyle evolution of wild marine bacteria
11. Eliminating germline lengthens fly lifespan, Brown study shows
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Life's smallest motor, cargo carrier of the cells, moves like a seesaw
(Date:5/21/2015)... -- According to a new market ... Services), by Applications (Surveillance (Airborne, Maritime, Land), Threat ... & Defense & Commercial) - Global Forecast to ... expected to grow from $7252.0 Million in 2015 ... Annual Growth Rate (CAGR) of 6.6%. ...
(Date:5/19/2015)... YORK , May 19, 2015  Technology is ... both internally and in the cloud. Passwords and their ... use of OTP and standards-based specifications such as those ... the outmoded use of passwords presents for BYOD, COPE, ... a unified biometric identity protocol. In ...
(Date:5/11/2015)... 2015  Synaptics Incorporated (NASDAQ: SYNA ), ... announced the appointment of Wajid Ali ... reporting to Rick Bergman , President and ... Officer, Kathleen Bayless , who announced her ... Ali brings extensive financial management expertise to Synaptics, ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2
... and the Veterans Affairs Greater Los Angeles Healthcare System ... the cost-effectiveness of current modern drugs for treating hepatitis ... Published in the May 17 Annals of Internal Medicine, ... decisions. Researchers note that a degree of uncertainly exists ...
... known for a long time that only 2% -- 3% ... of our genomes -- often referred to as junk DNA ... RNA, reverse-transcribed into DNA, and then reinserted into a new ... class of these retroelements. Retroviruses can insinuate themselves into the ...
... Don't miss out the cool movies, link at the end ... Medicine researchers have found a way to exercise a little ... their wings, and fly on command by triggering genetic "remote ... central nervous systems, according to a new report in the ...
Cached Biology News:UCLA study assesses cost-effectiveness of Hepatitis B drugs 2UCLA study assesses cost-effectiveness of Hepatitis B drugs 3Chimp genome reveals a retroviral invasion 2Chimp genome reveals a retroviral invasion 3Remote control flies? Fly behavior controlled by laser light 2Remote control flies? Fly behavior controlled by laser light 3
(Date:6/1/2015)... , June 1, 2015  Novartis is ... study of CTL019, an investigational chimeric antigen receptor ... in the treatment of specific types of hard-to-treat ... by the University of Pennsylvania,s Perelman School of ... (r/r) diffuse large B-cell lymphoma (DLBCL) and follicular ...
(Date:6/1/2015)... IL (PRWEB) June 01, 2015 ... developing and manufacturing of chiral stationary phases (CSPs) ... options for its current and presenting solutions for ... to its portfolio. This valuable addition to its ... and effective advantage that can tackle projects for ...
(Date:6/1/2015)... 1, 2015  Boston-based Veritas Genetics is launching ... and bring personalized medicine and prevention to the ... their health. Beginning with its first product, a ... risk, Veritas Genetics is making its products affordable ... amount of genetic data which will be shared ...
(Date:6/1/2015)... MD (PRWEB) June 01, 2015 ... they have entered into a commercial collaboration agreement ... companies will combine their technologies and work together ... (MSC) regenerative technology. RoosterBio and Sistemic will collaborate ... technologies to build more precise and relevant characterization ...
Breaking Biology Technology:Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 2Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 3Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 4Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 5Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 6Regis Technologies Offers Chiral Chromatography Service 2Regis Technologies Offers Chiral Chromatography Service 3Veritas Genetics To Advance Precision Medicine And Disease Prevention 2Veritas Genetics To Advance Precision Medicine And Disease Prevention 3Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 2Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 3Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 4
... Technologies LLC (Myriant), a privately-held, biotech developer and ... its Science Advisory Board and the appointment of ... will provide broad insight into all aspects of ... feedstocks into valuable chemicals. , (Logo: ...
... N.C., Oct. 21 Cornerstone Therapeutics Inc. (Nasdaq CM: ... and commercializing significant products primarily for the respiratory and ... conference call at 8:30 AM ET on Wednesday, November ... quarter ended September 30, 2009. Management will also ...
... ... business models capture investors, interest. , ... Miami, FL (PRWEB) October 21, 2009 -- iLife Americas, LLC, a ... in the U.S. to market its line of iLife wellness and therapeutic products. Officially ...
Cached Biology Technology:Myriant Technologies LLC Announces Appointment of Science Advisory Board Members 2Myriant Technologies LLC Announces Appointment of Science Advisory Board Members 3Cornerstone Therapeutics to Host Third Quarter 2009 Conference Call 2Cornerstone Therapeutics to Host Third Quarter 2009 Conference Call 3iLife Americas, LLC Enters U.S. Wellness and Therapeutic Markets 2
...
5 minutes from gel band to purified DNA...
Sterile concentration of Dithiothreitol (DTT) solution in phosphate buffer (pH 7.0). Dilute contents in one vial (10- fold) to 100 ml. For use in liquefying sputum samples....
...
Biology Products: