Navigation Links
Leiden scientists sequence first female DNA
Date:5/27/2008

Geneticists of Leiden University Medical Centre (LUMC) are the first to determine the DNA sequence of a woman. She is also the first European whose DNA sequence has been determined. This has been announced by the researchers this morning, during a special press conference at Bessensap, a yearly meeting of scientists and the press in the Netherlands.

Following in-depth analysis, the sequence will be made public, except incidental privacy-sensitive findings. The results will contribute to insights into human genetic diversity.

DNA of geneticist Marjolein Kriek

The DNA is that of dr Marjolein Kriek, a clinical geneticist at LUMC. If anyone could properly consider the ramifications of knowing his or her sequence, it is a clinical geneticist, says professor Gert-Jan B van Ommen, leader of the LUMC team and director of the Center for Medical Systems Biology (CMSB), a center of the Netherlands Genomics Initiative.

Van Ommen continues: Moreover, while women dont have a Y-chromosome, they have two X-chromosomes. As the X-chromosome is present as a single copy in half the population, the males, it has undergone a harsher selection in human evolution. This has made it less variable. We considered that sequencing only males, for completeness, slows insight into X-chromosome varialibity. So it was time, after sequencing four males, to balance the genders a bit. He smiles: And after Watson we also felt that it was okay to do Kriek.

Eight times coverage

The DNA sequencing was done with the Illumina 1G equipment. This has been installed in January 2007 in the Leiden Genome Technology Center, the genomics facility of LUMC and CMSB. In total, approx. 22 billion base pairs (the letters of the DNA language) were read. That is almost eight times the size of the human genome.

Dr. Johan den Dunnen, project leader at the Leiden Genome Technology Center: 'This high coverage is needed to prevent mistakes, connect the separate reads and reduces the chance of occasional uncovered gaps.

Johan den Dunnen: 'The sequencing itself took about six months. Partly since it was run as a side operation filling the empty positions on the machine while running other projects. Would such a job be done in one go, it would take just ten weeks.

The cost of the project was approximately 40.000.- This does not include further in-depth bioinformatics analysis. This is estimated to take another six months.

History

In 2001, the DNA sequence was published of a combination of persons. The DNA sequences of Jim Watson, discoverer of the DNAs double helix structure, followed in 2007, and later the DNA of gene hunter Craig Venter. Recently the completion of the sequences of two Yoruba-Africans was announced.

Bessensap

The researchers announced their news at the yearly Bessensap meeting, bringing together the Dutch scientists and the press. The Netherlands Organization for Scientific Research NWO organizes this event jointly with the Association of Science Writers VWN and Science Center NEMO. In its eight years of existence, Bessensap (www.nwo.nl/bessensap) has had several high-profile news items. It has had a debate with Italian clonedoctor Severino Antinori and hosted dino-hunter Jack Horner, who was key in the Jurassic-Park modelling. During Bessensap also the yearly Eureka prize is awarded for the best popular-scientific book and media production.


'/>"/>

Contact: LUMC Directorat Communication
info@lumc.nl
31-071-526-8005
Netherlands Organization for Scientific Research
Source:Eurekalert

Related biology news :

1. UK scientists working to help cut ID theft
2. Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes
3. Comet probes reveal evidence of origin of life, scientists claim
4. Scientists link fragile X tremor/ataxia syndrome to binding protein in RNA
5. Male elephants get photo IDs from scientists
6. Scientists retrace evolution with first atomic structure of an ancient protein
7. Muscle mass: Scientists identify novel mode of transcriptional regulation during myogenesis
8. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
9. Clemson scientists shed light on molecules in living cells
10. Scientists tackle mystery mountain illness
11. T. rex quicker than Becks, say scientists
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/30/2017)... ARLINGTON, Va. , June 30, 2017 /PRNewswire-USNewswire/ ... a leading developer and supplier of face and ... the ATA Featured Product provider program. ... created an innovative way to monitor a driver,s ... benefit greatly from being able to detect fatigue ...
(Date:6/14/2017)... , June 15, 2017  IBM (NYSE: IBM ) ... international tech event dedicated to developing collaboration between startups and ... on June 15-17. During the event, nine startups will showcase ... value in various industries. France ... the international market, with a 30 percent increase in the ...
(Date:5/16/2017)... 2017  Veratad Technologies, LLC ( www.veratad.com ), an ... identity verification solutions, announced today they will participate as ... 15 thru May 17, 2017, in Washington ... Center. Identity impacts the lives of ... quickly evolving digital world, defining identity is critical to ...
Breaking Biology News(10 mins):
(Date:8/15/2017)... ... August 15, 2017 , ... ... Asian exhibitions for analytical and scientific instruments. This year’s symposium, organized by the ... in Mass Spectrometry for Bioanalytical Applications.” This dynamic presentation will discuss novel ionization ...
(Date:8/15/2017)... ... August 15, 2017 , ... ... by various biotic and abiotic factors. During this educational webinar, participants will learn ... as well as gain a better understanding of how genomics is important for ...
(Date:8/14/2017)... ... 2017 , ... The Conference Forum has confirmed the one-day agenda ... September 6, 2017 at the Marriott Copley Place in Boston, MA. , Returning as ... Regulatory Strategy, Pfizer Innovative Research Lab, Pfizer, who leads 19 industry speakers in discussing ...
(Date:8/11/2017)... , ... August 11, 2017 , ... ... production, and, in particular, more natural alternatives to synthetic ingredients,” said Matt Hundt, ... Third Wave, with the established manufacturing presence and know-how of Biorigin will allow ...
Breaking Biology Technology: