Navigation Links
Leading RSV researcher publishes work at Le Bonheur Children's
Date:2/14/2013

Memphis, Tenn. Studies at Le Bonheur Children's Hospital in Memphis, Tenn., are advancing our understanding of how viruses, including RSV, replicate in humans, mutate to avoid the immune response and can be effectively treated.

John DeVincenzo, MD, medical director of Molecular Diagnostics and Virology Laboratories at Le Bonheur, and professor of Pediatrics and Microbiology, Immunology, and Molecular Biology at The University of Tennessee Health Science Center, has recently published three papers on this topic. DeVincenzo's lab is one of only two of its kind in the United States. His work has focused on respiratory syncytial virus (RSV), the most common cause of infant hospitalization, and his findings are leading the way towards the development of antiviral treatment strategies against the disease.

Summaries of the studies' findings include:

Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification. Nucleic Acids Research, 2012

Viruses change and mutate at rapid rates in virtually random fashion. These high mutation rates of viruses always occur while they replicate within their human hosts. Detecting these changes has been difficult, because mutations that do not help the virus survive and replicate are overwhelmed by mutations that do help. Therefore, it is necessary to be able to detect minute populations of viruses that have mutated and to differentiate these small subpopulations from the majority populations of these viruses. This study outlines new advanced methods and techniques to detect minute sub-populations of viruses within clinical samples obtained from humans. These new techniques can open up areas of research in these viruses and how they mutate to avoid detection and control by our immune systems. Researchers capture 96 to 100 percent of the viral protein-coding region of HIV, respiratory syncytial and West Nile viral samples from as few as 100 copies of viral RNA. Methods are scalable to large numbers of samples and capable of generating full or near full-length viral genomes from clone and clinical samples with low amounts of viral RNA, without prior sequence information and in the presence of substantial RNA contamination from the surrounding human cells.

Assessing modeled CO2 retention and rebreathing of a facemask designed for efficient delivery of aerosols to infants. International Scholarly Research Network, 2012

New medicines for infant lung diseases, such as RSV, might require deposition of these medicines directly into the lung by infants breathing an aerosol. However, efficient masks that allow the safe delivery of these aerosols without causing the infants to re-breathe their own exhaled CO2 have not previously been developed. The researchers developed a new infant aerosol delivery mask. They then evaluated the mask using computational fluid dynamic models to see if it would both efficiently deliver aerosols and whether there was retention of exhaled CO2. Because RSV (respiratory syncytial virus) primarily is an infant disease, this device could be used for delivery of aerosol treatments of RSV in infants and children.

The promise and progress of RNA interference-based antiviral therapy for respiratory viruses. Antiviral Therapy, 2012

In this invited review, DeVincenzo discusses the science behind RNA interference (RNAi) and the potential practical issues in applying this novel treatment technology against various respiratory viral diseases. DeVincenzo was the first to demonstrate that RNAi-based therapies could work in humans. One of his recent studies showed that RSV-infected lung transplant patients treated with the novel RNAi therapy improved long term lung function significantly better than did those treated with placebo, even when the patients were analyzed as long as six months later. This marks the first effective treatment of RSV in this vulnerable immune-suppressed population. Ongoing research directed by DeVincenzo is now moving experimental RSV therapies into children.


'/>"/>

Contact: Hillary Welton
hillary.welton@lebonheur.org
901-287-6030
Le Bonheur Children's Hospital
Source:Eurekalert

Related biology news :

1. Leading evolutionary scientist to discuss how genome of bacteria has evolved
2. Worlds leading coral experts to gather in Australia
3. Putting plants online: 4 leading botanical gardens to create first online catalog of all plants
4. EMBO welcomes 55 leading life scientists as members
5. Leading statistician receives national citation award
6. Leading childhood asthma group supports federal asthma action plan to reduce disparities
7. BiOptix Participates in Leading Life Sciences Event BioWest 2012
8. Team including UC Riverside entomologist honored for research leading to healthier potato chips
9. Aware Enables Automated Delivery of Radiation Dose Information Through Integration With Leading Radiology Reporting System
10. Discovery of pathway leading to depression reveals new drug targets
11. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/11/2017)... Fla. , April 11, 2017 ... and secure authentication solutions, today announced that it ... Intelligence Advanced Research Projects Activity (IARPA) to develop ... Thor program. "Innovation has been a ... IARPA,s Thor program will allow us to innovate ...
(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
(Date:3/30/2017)... ANGELES , March 30, 2017  On April ... Hack the Genome hackathon at Microsoft,s ... exciting two-day competition will focus on developing health and ... Hack the Genome is the ... been tremendous. The world,s largest companies in the genomics, ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... publication of a United States multicenter, prospective clinical study that demonstrates the ... diagnostic test capable of identifying clinically significant acute bacterial and viral respiratory ...
(Date:10/11/2017)... ... October 11, 2017 , ... At its national ... Dr. Suneel I. Sheikh, the co-founder, CEO and chief research scientist of Minnesota-based ... selected for membership in ARCS Alumni Hall of Fame . ASTER Labs ...
(Date:10/11/2017)... , ... October 11, 2017 , ... ... announced today it will be hosting a Webinar titled, “Pathology is going digital. ... Associates , on digital pathology adoption best practices and how Proscia improves lab ...
(Date:10/11/2017)... ... 2017 , ... A new study published in Fertility and ... in vitro fertilization (IVF) transfer cycles. The multi-center matched cohort study ... comparing the results from the fresh and frozen transfer cohorts, the authors of ...
Breaking Biology Technology: