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Lab in a drop
Date:5/6/2008

t droplet, the magnetic droplet is moved around like a clockwork, passing again and again through four different zones set to the temperatures necessary for PCR. Each cycle lasts 8 seconds. A fluorescence detector over one of the zones monitors the progress of the PCR (real-time PCR) and indicates whether the desired gene sequence is present and in what amount.

With their new PCR chip, the researchers were able to isolate 30 cells implanted with the genetic information for a green-fluorescing protein from 25 L of blood, concentrate them 100-fold, wash them, rupture them, and detect the gene for the green protein by real-time PCRall within just 17 minutes!


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Contact: Jrgen Pipper
jpipper@ibn.a-star.edu.sg
656-824-7157
Wiley-Blackwell
Source:Eurekalert  

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