Navigation Links
Lab in a drop
Date:5/6/2008

This release is available in German.

Analysis and diagnosis in a chip format are coming of age, but their practical application has been limited because until now, the sample usually had to be prepared separately and on a nonminiaturized scale. Jrgen Pipper and his team at the Institute of Bioengineering and Nanotechnology in Singapore want to change this. They have now developed a rapid test for genetic diagnosis that combines the preparation of biological samples with a polymerase chain reaction (PCR) on one chip. As they report in the journal Angewandte Chemie, the laboratory device for all steps in this system is a single drop containing magnetic nanoparticles, which is moved across the chip by a magnetic field.

PCR allows gene sequences to be duplicated and identifiedto identify a disease trigger, for example. In this process, the sample must cycle through a specific sequence of temperatures. Because of the slow heating and cooling processes, laboratory PCR usually takes several hours. The new chip PCR requires only minutes, including for the sample preparation.

In contrast to other chip-based methods, the actual sample, such as a drop of blood, can be placed directly on the PCR chip, where it is mixed with a drop that contains magnetic particles. These particles are equipped with antibodies on their surface, antibodies that bind specifically to the interesting cells in the blood. By moving a magnet underneath the chip, a droplet containing the bound magnetic particles is physically pulled out of the blood droplet and moved on to the next stationa droplet of washing liquid. The magnetic droplet is combined with the washing droplet and then pulled out again through movement of the magnet. Another droplet then delivers the enzymes and reagents necessary for cell disruption.

The last station is the PCR station. After combination with a reagent droplet, the magnetic droplet is moved around like a clockwork, passing again and again through four different zones set to the temperatures necessary for PCR. Each cycle lasts 8 seconds. A fluorescence detector over one of the zones monitors the progress of the PCR (real-time PCR) and indicates whether the desired gene sequence is present and in what amount.

With their new PCR chip, the researchers were able to isolate 30 cells implanted with the genetic information for a green-fluorescing protein from 25 L of blood, concentrate them 100-fold, wash them, rupture them, and detect the gene for the green protein by real-time PCRall within just 17 minutes!


'/>"/>

Contact: Jrgen Pipper
jpipper@ibn.a-star.edu.sg
656-824-7157
Wiley-Blackwell
Source:Eurekalert  

Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Lab in a drop
(Date:4/11/2017)... 2017 Research and Markets has announced the ... to their offering. ... eye tracking market to grow at a CAGR of 30.37% during ... Market 2017-2021, has been prepared based on an in-depth market analysis ... and its growth prospects over the coming years. The report also ...
(Date:4/5/2017)... , April 5, 2017  The Allen Institute ... Allen Cell Explorer: a one-of-a-kind portal and dynamic digital ... 3D imaging data, the first application of deep learning ... human stem cell lines and a growing suite of ... platform for these and future publicly available resources created ...
(Date:4/3/2017)... WASHINGTON , April 3, 2017 /PRNewswire-USNewswire/ ... single-cell precision engineering platform, detected a statistically ... cell product prior to treatment and objective ... highlight the potential to predict whether cancer ... prior to treatment, as well as to ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... 11, 2017 , ... Personal eye wash is a basic first aid supply for any work ... which eye do you rinse first if a dangerous substance enters both eyes? It’s one ... with its unique dual eye piece. , “Whether its dirt and debris, or an ...
(Date:10/11/2017)... ... 11, 2017 , ... Disappearing forests and increased emissions are the main causes ... each year. Especially those living in larger cities are affected by air pollution related ... the most pollution-affected countries globally - decided to take action. , “I knew I ...
(Date:10/10/2017)... ... October 10, 2017 , ... For the second time in ... STEM Mentoring Award. Representatives of the FirstHand program travelled to Washington, D.C. Tuesday, ... , US2020’s mission is to change the trajectory of STEM education in America ...
(Date:10/10/2017)... ... 10, 2017 , ... The Pittcon Program Committee is pleased ... scientists who have made outstanding contributions to analytical chemistry and applied spectroscopy. Each ... leading conference and exposition for laboratory science, which will be held February 26-March ...
Breaking Biology Technology: