Navigation Links
Lab-engineered kidney project reaches early milestone
Date:6/21/2012

WINSTON-SALEM, N.C. June 21, 2012 Regenerative medicine researchers at Wake Forest Baptist Medical Center have reached an early milestone in a long-term project that aims to build replacement kidneys in the lab to help solve the shortage of donor organs.

In proof-of-concept research published online ahead of print in Annals of Surgery, the team successfully used pig kidneys to make "scaffolds" or support structures that could potentially one day be used to build new kidneys for human patients. The idea is to remove all animal cells leaving only the organ structure or "skeleton." A patient's own cells would then be placed on the scaffold, making an organ that the patient theoretically would not reject.

While this is one of the first studies to assess the possibility of using whole pig kidneys to engineer replacement organs, the idea of using organ structures from pigs to help human patients is not new. Pig heart valves removed of cells have been used for more than three decades to provide heart valve replacements in human patients.

"It is important to identify new sources of transplantable organs because of the critical shortage of donor organs," said lead author Giuseppe Orlando, M.D., an instructor in surgery and regenerative medicine at Wake Forest Baptist. "These kidneys maintain their innate three-dimensional architecture, as well as their vascular system, and may represent the ideal platform for kidney engineering."

For the research, pig kidneys were soaked in a detergent to remove all cells, leaving behind the organ's "skeleton," including its system of blood vessels. In addition, the structure of the nephron the kidney's functional unit was maintained. The scaffolds were implanted in animals, where they were re-filled with blood and were able to maintain normal blood pressure, proving that the process of removing cells doesn't affect the mechanical strength of the vessels.

"There are many challenges to be met before this system could be used to engineer replacement kidneys, including problems with blood clots forming in the vessels," said Anthony Atala, M.D., co-author and director of the Wake Forest Institute for Regenerative Medicine. "The kidney is a very complex organ with at least 22 different cell types."

But, the fact that nephron structure is maintained suggests the potential to re-populate the kidney with cells, according to the scientists. They speculate that new cells introduced into the scaffold would recognize their natural niche through physical or chemical signals of the scaffold.

While the project is in its infancy, the idea represents a potential solution to the extreme shortage of donor kidneys. According to the authors, the probability in the U.S. of receiving a kidney transplant within five years of being added to the waiting list is less than 35 percent. As of late August 2011, nearly 90,000 patients in the U.S. were waiting for kidney transplants.

The science of regenerative medicine has already had success engineering skin, cartilage, bladders, urine tubes, trachea and blood vessels in the lab that were successfully implanted in patients. These structures were able to receive oxygen and nutrients from nearby vascularized tissues until they developed their own blood vessel supply.

However, the "holy grail" of regenerative medicine is to engineer more complex organs such as the kidney, liver, heart and pancreas. These organs are very dense with cells and must have their own oxygen supply to survive. This need for a scaffold with a full vasculature is why scientists are exploring the possibility of removing cells from donor organs and replacing them with a patient's own cells.

Scientists have already used scaffolds from rodents or pigs to engineer heart, liver, lung and intestinal scaffolds. When re-populated with organ-specific cells, these "organoids" were able to produce some of the functions of native organs in the lab. The goal of the current study is to produce kidney scaffolds from the pig because of similarities to humans in terms of organ structure and size.


'/>"/>
Contact: Karen Richardson
krchrdsn@wakehealth.edu
336-716-4453
Wake Forest Baptist Medical Center
Source:Eurekalert

Related biology news :

1. NIH supporting Arizona State University research to help in fight against kidney disease
2. Intensive kidney dialysis indicates better survival rates than conventional dialysis
3. Kidney stone mystery solved
4. UCSF artificial kidney project tapped for accelerated FDA program
5. BGI and the Childrens Hospital of Philadelphia launch the 1,000 Rare Diseases Project
6. IU role in Human Microbiome Project exposes battle history between bacteria, viruses in human body
7. Reed Elsevier Environmental Challenge shortlists 2012 projects
8. BGI, GE Healthcare team up on pioneering stem cell science projects
9. Southampton researchers lead 2 international projects to help people out of poverty
10. University leads £6 million EU project to tackle obesity
11. EU project: Searching for exotics in the shrimp nets
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/1/2017)... 1, 2017 IDTechEx Research, a leading provider ... announces the availability of a new report, Sensors for Robotics: ... Continue Reading ... ... robots. Source: IDTechEx Report "Sensors for Robotics: Technologies, Markets and Forecasts ...
(Date:1/26/2017)... , Jan. 26, 2017  Crossmatch, a leading provider ... new solution aimed at combatting fraud, waste and abuse ... introduced at the Action on Disaster Relief conference in ... point for UN agencies and foreign assistance organizations throughout ... waste and abuse are a largely unacknowledged problem in ...
(Date:1/24/2017)... Jan. 24, 2017 Biopharm Reports has ... laboratory use of nuclear magnetic resonance spectroscopy (NMR). ... and profiled current practices, developments, trends and end-user ... as growth and opportunities. These areas include growth ... instruments, needs and innovation requirements, hyphenated NMR techniques, ...
Breaking Biology News(10 mins):
(Date:2/28/2017)... YORK , February 28, 2017 ... for avelumab  Prognosis for urothelial carcinoma is currently ... EMD Serono, the biopharmaceutical business of ... US and Canada , and Pfizer Inc. ... has accepted for Priority Review EMD Serono,s Biologics License Application ...
(Date:2/27/2017)... , Feb. 27, 2017 AcelRx ... focused on the development and commercialization of innovative ... announced today the U.S. Food and Drug Administration ... (NDA) under section 505(b)(2) for DSUVIA™ (formerly ARX-04) ... of patients with moderate-to-severe acute pain in a ...
(Date:2/27/2017)... URBANDALE, Iowa (PRWEB) , ... February 27, 2017 , ... ... one of only a few in the Midwest offering premix services and private label ... poultry feed for laying hens, according Jos Zamzow, chief operating officer of Dynamite Marketing, ...
(Date:2/24/2017)... ... February 24, 2017 , ... Chef Jodi Abel has returned from her ... in several cities, she gained a number of delicious recipes and new techniques to ... in South Africa’s Western Cape province. It is internationally renowned for its incredible ...
Breaking Biology Technology: