In his Nature paper entitled "'Slings' enable neutrophil rolling at high shear," Dr. Ley revealed how neutrophils use sling-like membrane tethers to latch on to the blood vessel wall during periods when blood flow is very fast. In making the discovery, Dr. Ley and Prithu Sundd, Ph.D., a researcher at La Jolla Institute, used "dynamic footprinting," a pioneering imaging technique they developed in 2010 that uses special microscopes and total internal reflection microscopy to see and photograph the neutrophil adhesion process with unprecedented clarity. Alex Groisman, Ph.D., an associate professor in UCSD's Department of Physics, was instrumental in developing and constructing the microfluidic device in which these experiments were conducted and collaborated on the Nature paper.
Sussan Nourshargh, Ph.D., professor of Microvascular Pharmacology and head of the Center for Microvascular Research at Barts and The London Medical School, University of London, said the work provides another "major insight" from Dr. Ley whose discoveries, over the years, have repeatedly enhanced scientific understanding of the role of neutrophils in causing inflammation. In particular, she cited Dr. Ley's groundbreaking work on the discovery of the leukocyte adhesion cascade, which explained the sequential steps used by neutrophils to clamp onto the blood vessel wall as they prepare to migrate to sites of infection. His latest finding reveals another important step in that process.
"This is a completely new cellular concept that will now be added as an additional step to the leukocyte adhesion cascade that describes the sequential cellular responses involved in guiding neutrophils to sites of inflammation," she said. "This pioneering work will without doubt pave the way for other researchers to explore the occurrence of "slings" in a wide range of inflammatory scenarios."
Like other immune cells, neutrophils travel throughout the body via the blood s
|Contact: Bonnie Ward|
La Jolla Institute for Allergy and Immunology