Navigation Links
Kinked nanopores slow DNA passage for easier sequencing
Date:7/30/2010

ALBUQUERQUE, N.M. In an innovation critical to improved DNA sequencing, a markedly slower transmission of DNA through nanopores has been achieved by a team led by Sandia National Laboratories researchers.

Solid-state nanopores sculpted from silicon dioxide are generally straight, tiny tunnels more than a thousand times smaller than the diameter of a human hair. They are used as sensors to detect and characterize DNA, RNA and proteins. But these materials shoot through such holes so rapidly that sequencing the DNA passing through them, for example, is a problem.

In a paper published this week online (July 23) in Nature Materials (hardcopy slated for August, Vol.9, pp. 667-675), a team led by Sandia National Laboratories researchers reports using self-assembly techniques to fabricate equally tiny but kinked nanopores. Combined with atomic-layer deposition to modify the chemical characteristics of the nanopores, the innovations achieve a fivefold slowdown in the voltage-driven translocation speeds critically needed in DNA sequencing. (Translocation involves DNA entering and passing completely through the pores, which are only slightly wider than the DNA itself.)

"By control of pore size, length, shape and composition," says lead researcher Jeff Brinker, "we capture the main functional behaviors of protein pores in our solid-state nanopore system." The importance of a fivefold slowdown in this kind of work, Brinker says, is large.

Also of note is the technique's capability to separate single- and double-stranded DNA in an array format. "There are promising DNA sequencing technologies that require this," says Brinker.

The idea of using synthetic solid-state nanopores as single-molecule sensors for detection and characterization of DNA and its sister materials is currently under intensive investigation by researchers around the world. The thrust was inspired by the exquisite selectivity and flux demonstrated by natural biological channels. Researchers hope to emulate these behaviors by creating more robust synthetic materials more readily integrated into practical devices.

Current scientific procedures align the formation of nominally cylindrical or conical pores at right angles to a membrane surface. These are less capable of significantly slowing the passage of DNA than the kinked nanopores.

"We had a pretty simple idea," Brinker says. "We use the self-assembly approaches we pioneered to make ultrathin membranes with ordered arrays of about 3-nanometer diameter pores. We then further tune the pore size via an atomic-layer deposition process we invented. This allows us to control the pore diameter and surface chemistry at the subnanometer scale. Compared to other solid state nanopores developed to date, our system combines finer control of pore size with the development of a kinked pore pathway. In combination, these allow slowing down the DNA velocity."

The work is supported by the Air Force Office of Scientific Research, the Department of Energy's Basic Energy Sciences and Sandia's Laboratory Directed Research and Development office.


'/>"/>

Contact: neal singer
nsinger@sandia.gov
505-845-7078
DOE/Sandia National Laboratories
Source:Eurekalert

Related biology news :

1. First step toward electronic DNA sequencing: Translocation through graphene nanopores
2. NIST team advances in translating language of nanopores
3. Lollipops and ice fishing: Molecular rulers used to probe nanopores
4. Polymer passage takes time
5. Seabed biodiversity of the Straits of Magellan and Drake Passage
6. Technology improves salmon passage at hydropower dams
7. Seemingly suicidal stunt is normal rite of passage for immune cells
8. New retrieval method makes studying cancer proteins easier
9. Pesticides -- easier detection of pollution and impact in rivers
10. Grouping muscles to make controlling limbs easier
11. A little java makes it easier to jive, researcher says
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/7/2017)... , Feb. 7, 2017 Zimmer Biomet Holdings, ... musculoskeletal healthcare, will present at the LEERINK Partners 6th ... Palace Hotel on Wednesday, February 15, 2017 at 10 ... of the presentation can be accessed at http://wsw.com/webcast/leerink28/zbh ... the conference via Zimmer Biomet,s Investor Relations website at ...
(Date:2/6/2017)... DENVER , Feb. 6, 2017 ... national security are driving border authorities to continue ... Acuity reports there are 2143 Automated Border Control ... Kiosks currently deployed at more than 163 ports ... between 2013 to 2016 achieving a combined CAGR ...
(Date:2/3/2017)... , Feb. 3, 2017 A new ... Identity Strategy Partners, LLP (IdSP) . Designed to fill ... the complex identity market, founding partners Mark Crego ... 35 combined years just in identity expertise that span ... and non-profit leadership. The Crego-Kephart combined expertise has a ...
Breaking Biology News(10 mins):
(Date:2/28/2017)... ... February 28, 2017 , ... RoviSys, a leading independent provider ... has joined the Cisco® Digital Solutions Integrator Program. As a member of this ... that addresses Industrial IT and Business IT in manufacturing environments. , John ...
(Date:2/28/2017)... ... February 28, 2017 , ... Over 8,000 recognized rare diseases afflict an ... webinar on March 7, DrugDev will gather international experts to share challenges ... area. , Webinar: Overcoming the Challenges of Rare Disease Clinical Trials, Date: March ...
(Date:2/28/2017)... ... February 28, 2017 , ... ... therapeutics from millions-diverse immune repertoires, today announced a strategic partnership with Trianni, ... engineered to express human antibodies. The partnership will use GigaGen technology to discover ...
(Date:2/28/2017)... LONDON and PHILADELPHIA , ... Pharma"), a privately held biopharmaceutical company focusing on debilitating ... treatment options, today announced that the company joins with ... leaders in patient advocacy and support around the world ... to highlight the unique challenges and needs of the ...
Breaking Biology Technology: