It is important to demonstrate the influence of the microenvironment in the process of ...(producing the gene regulation expressed ...)
producing the gene regulation expressed by colon cancer cells during their growth as metastasis in the liver of patients with hepatic metastasis. On the other, it was aimed at identifying the origin of the factors regulating the expression of those genes.
An in vitro model based on crops in the presence of conditional media
The in vitro model developed in this PhD thesis differs from current models in which the cell line from the human colon cancer is cultured in the presence of conditioned media of primary cells from the liver after being stimulated with tumour cell conditioned media. With in vitro conditioned media, the aim is to imitate the environment of the tumour cell during its implantation in an organ at a distance. This would be the simplest form to see the changes and responses produced with this kind of metastasis, and subsequently being able to develop new pharmaceutical drugs or therapeutic targets to combat it.
To this end, liver cells - hepatocytes and myofibroblasts - are isolated, and both cultured separately in the presence of conditioned media of a cell line of colon cancer. In each case, the media are collected in such a way that all the segregated soluble factors of these liver cells, responding to the tumour, are in the media. On the other hand, tumoural cells from the human colon cancer cell line were cultured for 24 hours in the presence of this conditioned media, in order to see the response that these produce in each case and compare it with both the hepatocytes and myofibroblasts.
More than 44,000 genes
To check the responses of the colon cancer tumoural cells in the conditioned media, a study of the genes was carried out and the origin of the factors regulating this expression identified. Once the 24 hours in the presence of conditioned media had passed, the nucleic acid of these cells was extracted and this subsequently subjected to the microarrays technique enabling the study of more than
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| Contact: Irati Kortabitarte iratik@elhuyar.com 34-943-363-040 Elhuyar Fundazioa Source:Eurekalert |
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