Navigation Links
Is it a rock, or is it Jell-O? Defining the architecture of rhomboid enzymes
Date:8/2/2012

ly, and the temperature at which this occurs (in effect, the breaking point of the enzyme) indicates the inherent stability of the enzyme.

The researchers first precisely measured the stability of the rhomboid enzyme from E. coli bacteria. Surprisingly, says Urban, the rhomboid enzyme was more "Jell-O-like" than other membrane proteins with similar shapes. He guesses that this "jiggly shape" may help rhomboid proteases interact with other proteins that it cuts. To find which parts of the enzyme are most important for maintaining shape and which parts are more crucial for function, the researchers then made and tested 150 differently altered versions of the enzyme. They found four main regions important for maintaining shape and at least two regions important for function.

The researchers also took advantage of computer simulations to test their ideas about how the enzyme functions. Using a computer program model of the enzyme, they programmed in features of its natural membrane environment, which consists mostly of fats and is very limited in water. The computer program then simulated how this environment might influence the enzyme. Researchers found that the enzyme contains a special internal pocket for holding water molecules a great advantage in its natural, water-limiting environment.

"We're very excited about our findings and are especially curious about the versions of the enzyme that lost function despite no obvious change in stability or shape," says Urban. Ultimately he hopes that a better understanding of rhomboid proteases will lead to new therapies for treating malaria and other parasitic diseases.


'/>"/>
Contact: Vanessa McMains
vmcmain1@jhmi.edu
410-502-9410
Johns Hopkins Medical Institutions
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Force of nature: Defining the mechanical mechanisms in living cells
2. Herbivores select on floral architecture in a South African bird-pollinated plant
3. ORNL process improves catalytic rate of enzymes by 3,000 percent
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Is it a rock, or is it Jell-O? Defining the architecture of rhomboid enzymes
(Date:7/31/2014)... Two new meta-analyses involving tree nuts (almonds, Brazil nuts, ... were recently published in the online publications, British ... PLOS ONE (ii). The BMJ Open ... metabolic syndrome (MetS) criteria and showed that tree nut ... fasting blood glucose. The PLOS ONE article ...
(Date:7/30/2014)... Queen in Lewis Carroll,s Through the Looking Glass, ... University of Singapore improved a 35-year-old ecology model ... to millions of years. , The new model, ... the "Red Queen Effect," an evolutionary hypothesis introduced ... suggests that organisms must constantly increase their fitness ...
(Date:7/30/2014)... climate change on the world,s animal and plant ... to a new paper by a research team ... and other organizations. The authors claim that most ... when it comes to climate change. , ... on the "direct" threats of changing temperatures and ...
Breaking Biology News(10 mins):New international tree nut council study looks at nuts, diabetes and metabolic syndrome 2Classic Lewis Carroll character inspires new ecological model 2Classic Lewis Carroll character inspires new ecological model 3Conservation scientists asking wrong questions on climate change impacts on wildlife 2
... Old muscle got a shot of youthful vigor in a ... Berkeley, setting the path for research on new treatments for ... Parkinson,s diseases. In a new study to be published ... Nature , researchers identified two key regulatory pathways that ...
... The University of Delaware has received $400,000 from the ... of an ocean observing system for the Mid-Atlantic region, ... Cod, Mass., and out to the continental shelf. Nearly ... Mid-Atlantic coast. The initiative is part of the ...
... Pep Canadell from CSIRO and the Global Carbon Project, ... billions of tons of carbon dioxide (CO2) absorbed annually ... climate change mitigation worth hundreds of billions of dollars. ... stocks, challenges in quantifying changes in the size of ...
Cached Biology News:Stem cell researchers give old muscle new pep 2Stem cell researchers give old muscle new pep 3Stem cell researchers give old muscle new pep 4UD nets NOAA funding for Mid-Atlantic ocean observing 2Tropical forest sustainability: A climate change boon 2
(Date:7/31/2014)... VALLEY, Calif. , July 31, 2014 ... San Francisco Bay Area , has acquired ... University of Oxford,s commercialisation company. The potent anti-inflammatory activity ... Greaves at the University of Oxford,s Sir ... The company, which has raised over $1.5 million in ...
(Date:7/31/2014)... 31, 2014 Research and Markets ... Market 2014-2018" report to their offering. ... the study of the genetic material or genomes of ... basis for the discovery and development of diagnostic and ... health conditions. Genomics is supported by three major product ...
(Date:7/31/2014)... DIEGO and SHENZHEN, China ... announced today the purchase of an Irys™System ... organization. BGI selected BioNano,s platform to enable comprehensive exploration ... improved assemblies for various organisms of interest, including those ... will partner together to develop new methods for multiplexing ...
(Date:7/31/2014)... (PRWEB) July 31, 2014 Although ... of the first transcriptome-wide analyses appearing around 2008, ... assay for RNA measurement. In the last several ... more specific, work with smaller input amounts, process ... (eg, blood samples). On the bioinformatics side, there ...
Breaking Biology Technology:Oxford licenses anti-inflammatory peptides to San Francisco Bay Area start-up 2Oxford licenses anti-inflammatory peptides to San Francisco Bay Area start-up 3Global Genomics Market 2014-2018: Key Vendors are F. Hoffmann-La Roche, Life Technologies, Qiagen and Illumina 2BioNano Genomics Announces the Purchase of the Irys System by BGI 2BioNano Genomics Announces the Purchase of the Irys System by BGI 3BioNano Genomics Announces the Purchase of the Irys System by BGI 4The Evolution of RNA-Sequencing, Upcoming Live Webinar August 22 hosted by Xtalks 2
... pET vectors for bacterial expression has been the cost ... In order to tackle this problem, DNA2.0 has developed ... free of IP constraints. pJexpress vectors are specifically engineered ... shown to express better than comparable pET vectors. pJexpress ...
... US Oncology, Inc ., one of the nation,s largest cancer care ... Most Admired Companies list for 2009. The company ranks third ... third time US Oncology has been honored with F ... named to the list in 2004 and 2007 in ...
... a leading provider of centralized ECG and eClinical technology, ... and related industries, announced today that it will be ... #1253/1254 at the 21st Annual DIA Euromeeting, 23-25 March ... to three speaking sessions, which will discuss the impact ...
Cached Biology Technology:DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors 2DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors 3US Oncology Named to FORTUNE Magazine's Most Admired Companies List for Third Time 2US Oncology Named to FORTUNE Magazine's Most Admired Companies List for Third Time 3ERT to Showcase Its Clinical Trials Portfolio at DIA's 21st Euromeeting 2ERT to Showcase Its Clinical Trials Portfolio at DIA's 21st Euromeeting 3
... Detection Platform combines robust chemistry with ... sensitive, specific, and cost-effective detection method ... Platform offers exceptional performance with specificity ... high signal/background ratio, and an expanded ...
Request Info...
... Gel Band Purification Kit, 100. For the purification, ... fragments (0.1-10 kb) from solution and from TAE ... from solution in as little as 5 min ... min.Prepacked glass fiber matrix columns provide consistent results ...
BD BaculoGold Max-XP Insect Cell Medium 1 Liter...
Biology Products: