Navigation Links
Is it a rock, or is it Jell-O? Defining the architecture of rhomboid enzymes
Date:8/2/2012

ly, and the temperature at which this occurs (in effect, the breaking point of the enzyme) indicates the inherent stability of the enzyme.

The researchers first precisely measured the stability of the rhomboid enzyme from E. coli bacteria. Surprisingly, says Urban, the rhomboid enzyme was more "Jell-O-like" than other membrane proteins with similar shapes. He guesses that this "jiggly shape" may help rhomboid proteases interact with other proteins that it cuts. To find which parts of the enzyme are most important for maintaining shape and which parts are more crucial for function, the researchers then made and tested 150 differently altered versions of the enzyme. They found four main regions important for maintaining shape and at least two regions important for function.

The researchers also took advantage of computer simulations to test their ideas about how the enzyme functions. Using a computer program model of the enzyme, they programmed in features of its natural membrane environment, which consists mostly of fats and is very limited in water. The computer program then simulated how this environment might influence the enzyme. Researchers found that the enzyme contains a special internal pocket for holding water molecules a great advantage in its natural, water-limiting environment.

"We're very excited about our findings and are especially curious about the versions of the enzyme that lost function despite no obvious change in stability or shape," says Urban. Ultimately he hopes that a better understanding of rhomboid proteases will lead to new therapies for treating malaria and other parasitic diseases.


'/>"/>
Contact: Vanessa McMains
vmcmain1@jhmi.edu
410-502-9410
Johns Hopkins Medical Institutions
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Force of nature: Defining the mechanical mechanisms in living cells
2. Herbivores select on floral architecture in a South African bird-pollinated plant
3. ORNL process improves catalytic rate of enzymes by 3,000 percent
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Is it a rock, or is it Jell-O? Defining the architecture of rhomboid enzymes
(Date:1/22/2015)... Hall E -   EyeLock Inc. , a market leader ... its EyeLock ID technology integrated in a 3D printed automobile ... Ridge National Laboratory (ORNL) at the 2015 North ... is being used to validate the driver and authorize the ...
(Date:1/22/2015)... Conn. , Jan. 20, 2015 NXT-ID, Inc. (NASDAQ: ... mobile commerce market, reports on the recent success of the Wocket™ smart ... Wocket smart wallet was named as one of the "11 ... the "5 Best Products Launched At CES So Far" by Newseveryday.com and ...
(Date:1/22/2015)... , Europe,s most prestigious innovation ... to present a video retrospective of the most exciting and innovative ... Toumazou and Sophie Wilson   A different ...   Starting on 22 January 2015 on   http://www.epo.org/european-inventor   ...
Breaking Biology News(10 mins):EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3
... climatic trigger for many tree crops, is likely to ... as global climate warms, making California no longer suitable ... a team of researchers from the University of California, ... parts of California,s agriculturally rich Central Valley, winter chill ...
... may some day soon be driving on tires that are ... perform better and save on fuel and energy. Wood ... findings about the potential of microcrystalline cellulose a product ... plant fibers to partially replace silica as a reinforcing ...
... from other planets. So concludes a major scientific article which will ... Cosmology.com For thousands of ... Surprisingly, most scientists and the Catholic Church are of the same ... soup." In the Judeo-Christian Bible, the story ...
Cached Biology News:Warming climate threatens California fruit and nut production 2Warming climate threatens California fruit and nut production 3Tires made from trees -- better, cheaper, more fuel efficient 2Life on Earth came from other planets 2Life on Earth came from other planets 3Life on Earth came from other planets 4
(Date:12/24/2014)... GMO corn cases filed across the United States, ... of being consolidated in a Kansas federal court for pretrial ... 162 Corn Litigation, MDL No. 2591 in the U.S. District ... Syngenta GMO corn multidistrict litigation (MDL) has been handed over ...
(Date:12/24/2014)... BOSTON, MA (PRWEB) December 23, 2014 ... series is designed to introduce cutting edge communication ... partners of platform upgrades. This webinar series demonstrates ... their quest to leverage web conferencing’s most innovative ...
(Date:12/24/2014)... Island, New York (PRWEB) December 23, 2014 ... and the Long Island Affiliate of ITRA Global, the national ... slow but steady recovery. This is evidenced by the ... the strong stock market. Low energy costs have held ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 Nashville Fertility Center ... treasurer for the American Society for Reproductive Medicine (ASRM), a ... reproductive medicine. In his new role as treasurer, Dr. ... will have a voice in furthering the mission of ASRM. ...
Breaking Biology Technology:Carey Danis & Lowe Reports on Syngenta GMO Corn Transfer Order 2SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 3
... NanoSensors,Inc. (OTC Bulletin Board: NNSR) today announced ... President, Chief Financial Officer and Chairman of,the Board, ... separation,agreement with Dr. Wong dated September 9, 2007, ... positions with NanoSensors, including as a member,of the ...
... ANN ARBOR, Mich., Sept. 13 MichBio, ... issued its 2007/8 Michigan Life,Sciences Directory and ... state,s,life sciences companies. The guide includes editorial ... resources, the University,Research Corridor, the Michigan Universities ...
... Symposium Brings Together Thought Leaders,in Life Sciences and ... OOSTERHOUT, Netherlands, Sept. 13 Luminex,Corporation (Nasdaq: ... solutions,today announced the agenda for Planet xMAP Europe ... two-day event will be,held 2-3 October at the ...
Cached Biology Technology:NanoSensors, Inc. Announces Reorganization of Executive Management Team and Redirection of Operations 2NanoSensors, Inc. Announces Reorganization of Executive Management Team and Redirection of Operations 3NanoSensors, Inc. Announces Reorganization of Executive Management Team and Redirection of Operations 4NanoSensors, Inc. Announces Reorganization of Executive Management Team and Redirection of Operations 5Luminex Corporation Hosts the Fifth Annual Planet xMAP Europe 2007 Symposium 2Luminex Corporation Hosts the Fifth Annual Planet xMAP Europe 2007 Symposium 3
Preparation Note: Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948). Quality Clarifier: Contains lipase Unit Definition: One unit will hydrolyze 1.0 μmole of p-ni...
... Biuret Physical form: Solution in 50% glycerol ... mM MgCl 2 and 0.1 mM ... unit will hydrolyze 1 μmole of 4-nitrophenyl ... 37 C. • Concentration: ≥ 1,000 DEA ...
... Monitoring Kit measures adenosine triphosphate ... and a dynamic range. Based ... assay measures ATP in bacterial, ... a concentration range of 10-11 ...
... The Jouan RCT series of refrigerated ... suited to trapping of low volatility/aqueous solvents ... with RC10 and other vacuum concentrators/centrifugal evaporators. ... in cooling systems such as used in ...
Biology Products: