The researchers performed numerous experiments to test the growth rate of hybridoma cells in NPD-based media. They specifically tested the effect of the NPD environment on the complete process of human monoclonal antibody production, and their results were published recently in the BMC Biotechnology Journal.
To evaluate the hybridoma formation process (utilizing the chemical fusion method), the researchers received samples of human peripheral blood mononuclear cells (PBMC) from several donors; each sample was tested either in a NPD or a DI (de-ionized) environment. In referring to the results, the researchers stated: We witnessed a statistically significant difference in the yield of hybridoma cells between NPD and DI environments.
In another experiment, the isolation of subclones and autocrine activity of hybridoma cells was tested. The researchers reported: We observed greater clonal outgrowth of antibody-secreting hybridoma cells in NPD-based media as compared to DI-based media. Moreover, they found that the cloneability of cells from a semi-stable clone is also enhanced in NPD-based media.
The researchers noted that hybridoma clones grown on NPD-based media secreted more monoclonal antibodies into their environment. However, they also observed that Some cells grow faster in NPD-based mediaThis result might not reflect greater secretion per cell, but rather greater proliferation of cells with a similar secretion. After normalization of this biased situation, the researchers calculated that the secretion of monoclonal antibody in NPD-based media is roughly
|Contact: Irit Gabbai|
Do-Coop Technologies Ltd.